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Strain 1LN2 for preventing and treating rice sheath blight disease and application of strain 1LN2

A rice sheath blight, 1LN2 technology, applied in the direction of application, bacteria, fungicides, etc., to achieve the effect of improving the control effect, saving expenses, and increasing income

Inactive Publication Date: 2016-02-10
江苏省安丰生物源农药工程中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, no ideal immune or high-resistant varieties have been found, mainly relying on chemical agent control, and the environmental pollution problem is becoming more and more serious. Therefore, in today's emphasis on the development of green production technology, biological control, as a new idea for the prevention and treatment of rice sheath blight, will get further attention and development

Method used

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  • Strain 1LN2 for preventing and treating rice sheath blight disease and application of strain 1LN2
  • Strain 1LN2 for preventing and treating rice sheath blight disease and application of strain 1LN2
  • Strain 1LN2 for preventing and treating rice sheath blight disease and application of strain 1LN2

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Screening and Identification of Example 1 Bacterial Strains

[0024] In October 2011, samples were collected in Donglin Village, Chengxiang Town, Taicang City, Jiangsu Province, and the plots in the Science and Education New City with severe disease. The five-point sampling method is adopted, and the interval between each point is at least 10m. Three healthy plants per point were collected together with severely diseased plants together with rhizosphere soil. The collected samples were quickly put into numbered plastic bags and brought back to the laboratory for separation.

[0025] Strain 1LN2 was isolated from the stems of diseased plants. The separation method is as follows: respectively cut off 3 g of roots, stems and leaves, soak in 1 mL of 1% sodium hypochlorite for 5 minutes, soak in 70% alcohol for 2 minutes, and wash with sterile water for 3 times. (Take 0.1 mL of the last cleaning solution and apply it to the R2A plate, or take 0.1 mL of the last cleaning s...

Embodiment 2

[0032] Embodiment 2: the preparation of biocontrol agent

[0033] Streak the strain 1LN2 on the LB plate, and culture it in a biochemical incubator at 28°C for 14-16h. After a single colony grows, pick a single colony and put it into a test tube containing 5mL of LB culture medium, and place it at 28°C, 200r Cultivate in a shaker / min to make a seed solution; inoculate the seed solution with 1% inoculum in a conical flask containing 500mL of LB culture solution, and culture it in a shaker at 28°C and 200r / min for 48h , when the bacterial concentration reached 10 9 When CFU / mL is diluted for use, it is the prepared 1LN2 biocontrol agent.

Embodiment 3

[0034] Example 3: Greenhouse Control Effect Experiment and Greenhouse Growth Promotion Test

[0035] 1. Greenhouse control effect experiment:

[0036] Disinfect the rice seeds with 2% sodium hypochlorite solution for 15 minutes, rinse them with clean water for 3-4 times, soak them in a 30°C incubator for 48 hours, take them out, rinse them with clean water, and place the seeds that have absorbed enough water at 35 Cover the room at -38°C with gauze for high-temperature moisturizing and germination acceleration until the chest is broken. After the chest is broken, spread the buds in a cool place for 4h-8h. After the seeds are wet inside and dry outside, they can be sown and raised. Using the method of raising seedlings and transplanting, first sow the seeds in a seedling tray equipped with a sterilized substrate, and then place them in a room with a humidity of 85% or more at 30°C-35°C for darkening and emergence, and place them at 28°C- Cultivate in a greenhouse at 30°C, 16 / 8...

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Abstract

The invention discloses a strain 1LN2 for preventing and treating a rice sheath blight disease. The classification and nomenclature of the strain 1LN2 are pseudomonas sp., and the strain 1LN2 is preserved with the preservation number CGMCC No.11361 in China General Microbiological Culture Collection Center on September 10, 2015. The invention further discloses a biocontrol agent prepared from the strain 1LN2 for preventing and treating the rice sheath blight disease. The invention further discloses application of the biocontrol agent to prevention and treatment of the rice sheath blight disease. The biocontrol agent used in a greenhouse is capable of preventing and treating the rice sheath blight disease to a certain extent, greenhouse control effect is up to 60.25%, and field control effect after field experiment treatment is performed for one month is up to 50.31%; a 1LN2 strain preparation is capable of increasing protein content of rice, decreasing amylose content, improving gel consistency and increasing rice yield to a certain degree, and the yield increase rate obtained through the 1LN2 strain preparation reaches 7.1% compared with that obtained through a conventional chemical agent.

Description

technical field [0001] The invention belongs to the technical field of crop prevention and control, and specifically relates to a bacterial strain 1LN2 for preventing and treating rice sheath blight and an application thereof, which is specially used for green prevention and control of rice sheath blight. Background technique [0002] Rice sheath blight is a soil-borne disease caused by Rhizoctonia solani. It is one of the three major diseases of rice. It is distributed in various rice regions in the world and generally causes 10%-30% yield loss. When it is serious, it can cause a loss of production of more than 50%. In recent years, with the large-scale popularization of dwarf varieties and hybrid rice and the transformation of cultivation measures, the area of ​​rice sheath blight has increased year by year, which has brought great obstacles to the increase and stability of rice production. [0003] At present, the prevention and control of rice sheath blight has not yet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
Inventor 何胥施洁君王光
Owner 江苏省安丰生物源农药工程中心有限公司
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