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A kind of biocontrol strain yp1 for preventing and treating wheat take-all and its application

A technology of wheat take-all and biocontrol strains, applied in the fields of application, bacteria, and biocides, can solve the problems of ineffective control and environmental pollution, and achieve the effects of improving grain quality, saving expenses, and increasing income

Inactive Publication Date: 2011-12-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For a long time, the control of wheat take-all at home and abroad mostly started from agricultural control and chemical control, the control effect was not obvious, and it caused a certain degree of environmental pollution

Method used

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  • A kind of biocontrol strain yp1 for preventing and treating wheat take-all and its application
  • A kind of biocontrol strain yp1 for preventing and treating wheat take-all and its application
  • A kind of biocontrol strain yp1 for preventing and treating wheat take-all and its application

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Experimental program
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Effect test

Embodiment 1

[0018] The separation of embodiment 1YP1

[0019] (1) Strain YP1 is a strain of Pseudomonas fluorescens isolated on the leaf surface of wheat in June 2008. The isolation process is as follows: At the maturity stage of wheat, take several strains of wheat and put them into sterilized plastic bags and bring them back to the laboratory quickly. Put 5g of wheat leaves into a sterilized Erlenmeyer flask, add 45mL of sterile 0.85% NaCl, 5g of sterilized glass beads, vibrate on a shaker at 120-150rpm for 30min, then let stand for 5min to obtain a 10-1 dilution, draw Add 0.1mL to 0.9mL sterile 0.85% NaCl and mix well to get 10 -2 Diluent, and so on, can prepare 10 -3 , 10 -4 , 10 -5 , 10 -6 Diluent. 10 -4 , 10 -5 , 10 -6 Aspirate 0.1mL of the dilutions of the three gradients and inoculate them on the KMB selective medium, repeat each gradient three times, and separate and purify the colonies according to the size and shape of the colonies on the medium. Under the ultraviolet ...

Embodiment 2

[0024] The preparation of embodiment 2YP1 biocontrol agent

[0025] The biocontrol strain YP1 (CGMCC NO.4922) was shaken and cultivated in LB culture medium at 28°C and 180rpm for 12-16h, then centrifuged at 6000rpm for 10min, then centrifuged at 6000rpm for 10min, and diluted with sterilized water to make bacterial agent. The total concentration of bacteria is 1×10 9 -1×10 10 CFU / mL.

Embodiment 3

[0026] Embodiment 3 greenhouse pot test detects the control effect of YP1 biocontrol fungicide on wheat take-all

[0027] The YP1 biocontrol bacterium agent (YP1 CGMCC NO.4922 live bacteria concentration that prepares with embodiment 2 method is 10 8 CFU / mL) to treat wheat seeds by soaking for 30 minutes; meanwhile, 1.2% of wheat take-all bacteria were directly mixed into the soil. In addition, the treatment of direct inoculation of wheat take-all (Shanghai Fuzhong (Apennines) Biotechnology Development Co., Ltd.) without biocontrol agents was used as a control treatment. 21 strains in each treatment group, repeated three times. Under the conditions of temperature 20-25°C, relative humidity 40%, and light 12h / 12h, the disease progression was investigated after 4 weeks of cultivation, and the disease severity and control effect were calculated.

[0028] The percentage of the diseased root area in the total root area is used as the severity index, and the severity is divided in...

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Abstract

The invention belongs to the technical field of plant protection, disclosing a biocontrol bacterial strain YP1 for preventing and curing wheat takeall and an application thereof. The biocontrol bacterial strain YP1 is Pseudomonas fluorescens and is reserved in the China General Microbiological Culture Collection Center on June 1, 2011, and the bacterial strain collection number is CGMCC (China General Microbiological Culture Collection Center) No.4922. In the biocontrol bactericide prepared with the biocontrol bacterial strain YP1, the total concentration of the viable bacteria of the YP1 is 1*10<9>-1*10<10> CFU (colony-forming unit) / mL. The biocontrol bacterial strain can be used for preventing and curing the wheat takeall.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a biocontrol strain YP1 for preventing and treating wheat take-all and its application. Background technique [0002] Wheat take-all is a serious root disease caused by the wheat variety Gaeumannomyces graminis var.tritici (Ggt), which occurs in all major wheat producing areas in the world. Wheat take-all can occur at both seedling and mature stages, and the symptoms at the seedling stage are mild, while the symptoms at the adult plant stage are the most obvious. The diseased plants at the seedling stage are short, with many yellow leaves in the lower part, and the seed roots and underground stems turn gray-black, and in severe cases, the wheat seedlings die in pieces. So far there is no effective means of prevention and control. Wheat is an important food crop in my country. Wheat take-all disease was reported as early as the 1930s. In recent years, the incidence has gr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/39
Inventor 郭坚华杨明明刘红霞
Owner NANJING AGRICULTURAL UNIVERSITY
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