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Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof

A technology of tobacco black shank and organic fertilizer, which is applied in the field of intensive agricultural production, can solve the problems of low survival rate, loss of nutrient resources, waste of raw materials, etc., and achieves the effects of high biological control rate, remarkable inhibition effect and income increase.

Inactive Publication Date: 2010-04-21
禧鼎科技集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the temperature is low, although the seedlings can be infected by black shank bacteria, they often do not show symptoms. These tobacco seedlings that have been infected but have not yet shown symptoms are transplanted to the field. Not only the survival rate is very low, but also the initial invasion One of the sources of contamination
[0003] On the other hand, the incineration of rice, wheat, corn, rapeseed and other crop straws, and the disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste the organic fertilizer and bio-organic fertilizer products. Raw materials; a large number of nutrient resources (C, N, P, K, S and trace elements) are lost outside the soil-plant system, which obviously weakens the ability of my country's agricultural sustainable development

Method used

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  • Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof
  • Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof
  • Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof

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Embodiment Construction

[0023] (1) Isolation and identification of strains

[0024] The plants and surrounding soil that had suffered from continuous cropping obstacles and severe blackleg symptoms were collected, and the samples were stored at low temperature, and the pathogen Phytophthora parasitica var.nicotianae was isolated using a selective medium. Then the antagonistic bacteria were isolated from the rhizosphere soil of healthy tobacco plants from the continuous cropping tobacco black shank disease field with black shank pathogenic bacteria as indicator bacteria. Finally, through the pot test, the high-efficiency antagonistic strain C-5 was finally screened out, and the genus was identified. The strain was stored at -70°C in 30% glycerol. The antibacterial circle diameter of antagonistic bacteria C-5 is 28mm ( figure 1 ).

[0025] Strain C-5 belongs to Paenibacillus polymyxa, the main biological characteristics are: Gram stain positive G + , the cell shape is rod-shaped, the spore is oval, ...

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Abstract

The invention relates to an antagonistic bacterium for preventing and killing off tobacco black shank and a microbial organic fertilizer thereof, belonging to a production technology of intensive agriculture. An antagonistic bacterium (paenibacillus polymyxa C-5) having obvious antagonistic effect on the tobacco black shank pathogenetic bacteria is separated and utilized to prepare into the microbial organic fertilizer with organic compost, wherein the microbial organic fertilizer respectively contains more than 1*108 C-5 / g, the total-N content is 4-5 percent (more than 90 percent is organic nitrogen), the total nutrient content of nitrogen, phosphorus and potassium is 6-10 percent, and the content of organic matters is 30-35 percent. Proved by experiments, the microbial organic fertilizer enables the antagonistic bacterium to quickly breed and form a preponderant group in soil after being applied to the soil. The microbial organic fertilizer can greatly prevent the continuous cropping tobacco black shank if being applied to soil without continuous cropping obstacles for a long time.

Description

1. Technical field [0001] The invention relates to antagonistic bacteria capable of preventing continuous cropping tobacco black shank and a microbial organic fertilizer made from the same, which belongs to the technical field of agricultural intensive production and is specially used for overcoming and eliminating the problem of continuous cropping tobacco black shank. 2. Background technology [0002] Tobacco is the main economic crop in my country, and its quality is related to the national economy and people's livelihood. Tobacco black shank (Phytohthora parasitica var. nicotianae), found in Java, Indonesia, is an important fungal disease in tobacco production. Due to the rapid formation and large number of asexual propagules (spore sacs), the incubation period after infection is short, and the onset is rapid. Multiple infections can be carried out during the plant growth season. When the environmental conditions are suitable, it can spread over a large area in a short t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00C05G3/00C05G3/04C05F17/00C12R1/12C05G3/80
CPCY02W30/40
Inventor 沈其荣曹明慧杨兴明冉炜沈标
Owner 禧鼎科技集团有限公司
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