Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof
A technology of tobacco black shank and organic fertilizer, which is applied in the field of intensive agricultural production, can solve the problems of low survival rate, loss of nutrient resources, waste of raw materials, etc., and achieves the effects of high biological control rate, remarkable inhibition effect and income increase.
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[0023] (1) Isolation and identification of strains
[0024] The plants and surrounding soil that had suffered from continuous cropping obstacles and severe blackleg symptoms were collected, and the samples were stored at low temperature, and the pathogen Phytophthora parasitica var.nicotianae was isolated using a selective medium. Then the antagonistic bacteria were isolated from the rhizosphere soil of healthy tobacco plants from the continuous cropping tobacco black shank disease field with black shank pathogenic bacteria as indicator bacteria. Finally, through the pot test, the high-efficiency antagonistic strain C-5 was finally screened out, and the genus was identified. The strain was stored at -70°C in 30% glycerol. The antibacterial circle diameter of antagonistic bacteria C-5 is 28mm ( figure 1 ).
[0025] Strain C-5 belongs to Paenibacillus polymyxa, the main biological characteristics are: Gram stain positive G + , the cell shape is rod-shaped, the spore is oval, ...
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