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Biocontrol strain for preventing and controlling fusarium wilt of watermelon and application thereof

A watermelon fusarium wilt and bio-control strain technology is applied in the field of watermelon fusarium wilt biological control, which can solve the problems of watermelon wilt, watermelon quality reduction, and long duration of chemical pesticides, so as to increase farmers' income, save farmers' expenses, The effect of improving the quality of watermelon

Inactive Publication Date: 2014-10-08
NANJING XINGUOYUAN ECOLOGICAL AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the prevention and treatment of watermelon wilt mainly adopts the method of grafting pumpkin rootstock, which seriously affects the taste of watermelon and reduces the quality of watermelon
In view of the fact that watermelon wilt is widely distributed, serious damage, difficult to control, various disadvantages of chemical pesticides, and the development of chemical pesticides takes a long time and costs a lot, it is urgent to develop and commercially produce biological pesticides for the prevention and treatment of watermelon wilt

Method used

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  • Biocontrol strain for preventing and controlling fusarium wilt of watermelon and application thereof
  • Biocontrol strain for preventing and controlling fusarium wilt of watermelon and application thereof
  • Biocontrol strain for preventing and controlling fusarium wilt of watermelon and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening method for biocontrol strains of watermelon fusarium wilt

[0021] The biocontrol strain for controlling watermelon wilt, Bacillus subtilis (Bacillus subtilis) XGY13-1, was isolated from soil in diseased fields in Nanjing, Jiangsu in 2013. The screening process is as follows:

[0022] In May 2013, samples were collected from plots with severe incidence of watermelon wilt in Lishui District, Nanjing City, Jiangsu Province. The five-point sampling method is adopted, and the interval between each point is at least 10m. The rhizosphere soil of three healthy plants was collected at each point and brought back to the laboratory for isolation.

[0023] Weigh 3g of rhizosphere soil, add to 27mL sterilized water (containing sterilized glass beads) respectively, vibrate on a shaker at 180rmp for 0.5h, and let stand for 5min to obtain 10 -1 Diluent, absorb 0.1mL supernatant and dilute in turn, take 10 -4 ---10 -6 Three gradient 0.1mL plates were coated with...

Embodiment 2

[0026] Embodiment 2 strain identification

[0027] The biocontrol strain used to prevent and control watermelon wilt, Bacillus subtilis (Bacillus subtilis) XGY13-1 is a Gram-positive bacillus with a single cell of 0.7-0.8×2-3 microns and uniform coloring. Uncapsulated, with periosteum flagella, able to move. The spores are 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center or slightly off the thallus, and the thallus does not expand after spore formation. The surface of the colony is rough and opaque, stained white or yellowish, and often forms wrinkles when growing in LB liquid medium. Aerobic bacteria. It can use protein, various sugars and starch to decompose tryptophan to form indole.

Embodiment 3

[0028] Example 3 Preparation of XGY13-1 biocontrol agent

[0029] LB liquid medium is: yeast powder 5g / L, tryptone 10g / L, sodium chloride 10g / L, adjust pH 7.0-7.2.

[0030] The fermentation liquid is: soybean flour 10g / L, corn flour 75g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate heptahydrate 0.2g / L, yeast extract: 0.4g / L, sodium chloride 5g / L.

[0031] The isolated XGY13-1 single colony was picked into LB liquid medium, shaken and cultivated at 180 rpm at 30°C for 10 hours to obtain seed liquid. Inoculate the seed liquid with an inoculum of 1% on the above-mentioned fermentation medium, shake and culture at 30°C and 180rpm for 8-9 days, and the total concentration of viable bacteria in the finished bacterial preparation is 1×10 9 -1×10 10 CFU / mL.

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Abstract

The invention discloses a biocontrol strain for preventing and controlling fusarium wilt of watermelon and application thereof. The biocontrol strain is classified and named as Bacillus subtilis XGY13-1, is preserved in China General Microbiological Culture Collection Center and has the preservation number of CGMCC NO. 8973. The single colony of the biocontrol strain for preventing and controlling fusarium wilt of watermelon is picked into an LB liquid culture medium and is subjected to shaking culture on a 180rpm shaker at 30 DEG C for 10 hours to obtain a seed liquid, and the liquid seed is inoculated onto a fermentation culture medium at an inoculation amount of 1% (v / v) and subjected to shaking culture on a 180rpm shaker for 8-9 days at 30 DEG C to prepare a biocontrol microbial agent. The application method comprises the following steps: diluting the biocontrol microbial agent by 20-100 times, soaking watermelon seeds when the watermelon is planted or carrying out irrigating root treatment when watermelon seedlings are transplanted, wherein the fusarium wilt of watermelon is prevented and controlled by 62.36%. Meanwhile, the microbial agent has a growth-promoting function.

Description

technical field [0001] The invention relates to a biocontrol bacterial strain for preventing and treating watermelon wilt and an application thereof, which belongs to the technical field of plant protection and is specially used for biological control of watermelon wilt. Background technique [0002] Watermelon wilt is a disease of the vascular system caused by Fusarium oxysporum schlechlendahl f.sp.melons (Leach at currence) snyder et Hansen. One of the main diseases, since it was first discovered in South Carolina, USA in 1894, it has occurred in various watermelon producing areas in the world and at any time of watermelon growth, seriously threatening the safe production of watermelon. [0003] Fusarium wilt of watermelon can occur from seedlings to adult plants, and the onset is the most serious in the melon-setting stage and the later stage of melon expansion. Damaged seedlings can cause rotten seeds before emergence; after emergence, the cotyledons and true leaves are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 张磊晏枫霞韩先旭卢魏魏
Owner NANJING XINGUOYUAN ECOLOGICAL AGRI SCI & TECH
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