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Antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and microbial organic fertilizer thereof

A cotton verticillium wilt and organic fertilizer technology, applied in the field of intensive agricultural production, can solve the problems of waste of raw materials, pollution of the environment, loss of nutrient resources, etc., and achieve the effect of increasing income and saving expenses

Active Publication Date: 2010-03-17
河南省全元农业科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] On the other hand, incineration of rice, wheat, corn, rapeseed and other crop stalks and the disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste raw materials that can be used as organic fertilizers; a large amount of nutrients Resources (C, N, P, K, S and trace elements) are lost outside the soil-plant system, which obviously weakens the ability of my country's agricultural sustainable development

Method used

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  • Antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and microbial organic fertilizer thereof
  • Antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and microbial organic fertilizer thereof
  • Antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and microbial organic fertilizer thereof

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Embodiment Construction

[0026] (1) Isolation and identification of strains

[0027] Verticillium dahliae Kleb (Verticillium dahliae Kleb), the causative agent of Verticillium dahliae in cotton, was obtained from the China Culture Collection Center for Microorganisms (CGMCC No.3.3757). Antagonistic bacteria were isolated from the rhizosphere soil of healthy cotton plants in wilt-affected fields. Finally, through the pot experiment, the high-efficiency antagonistic strains NJHJ-5 and NJDF-14 were finally screened out, and the genera and species were identified. The strains were stored at -70°C in 30% glycerol. The inhibitory rate of antagonistic bacteria NJHJ-5 bacterial strain to pathogenic bacteria colony was 76.2%, and the inhibitory rate of NJDF-14 bacterial strain to pathogenic bacterial colony was 64.3% ( figure 1 , figure 2 ).

[0028] Strain NJHJ-5 belongs to Bacillus subtilis, the main biological characteristics are: Gram stain positive G + , the cell shape is rod-shaped, the spores are m...

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Abstract

The invention relates to antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and a microbial organic fertilizer thereof, belonging to a technology of agricultural intensification production. In the invention, two antagonistic bacteria (bacillus subtilis NJHJ-5 and bacillus subtilis NJDF-14) having obvious antagonistic actions on pathogenic bacteria of the cotton greensickness are separated, and the microbial organic fertilizer is prepared by utilizing the antagonistic bacteria and organic composts, wherein the microbial organic fertilizer respectively contains more than 1*10<9> / g of the NJHJ-5 and the NJDF-14, the total-nitrogen content is 4-5 percent (more than 90 percent of the content is organic nitrogen), total nitrogen-phosphorus-potassiumnutrients are 6-10 percent, and the content of organic matters is 30-35 percent. Proved by experiments, after being fertilized into soil, the microbial organic fertilizer enables the antagonistic bacteria to quickly reproduce and form a dominant group in the soil. After the microbial organic fertilizer is continuously fertilized for three seasons, the prevention and cure rate of the cotton greensickness in the soil the original morbidity of which is higher than 60 percent can reach 100 percent.

Description

1. Technical field [0001] The invention relates to antagonistic bacteria capable of preventing and eliminating Verticillium wilt in continuous cropping cotton and microbial organic fertilizer made by using the antagonistic bacteria, which belongs to the technical field of agricultural intensive production and is specially used to overcome and eliminate the problem of Verticillium wilt in continuous cropping cotton. 2. Background technology [0002] Cotton (Gossypium spp.) is an important economic crop and a natural raw material for the textile industry. At present, Coton Verticillium Wilt (Coton Verticillium Wilt) is one of the diseases that threaten cotton yield the most among cotton diseases. It is a vascular bundle disease that spreads through soil. In 1914, Carpenter first reported the discovery of cotton verticillium wilt in Virginia, USA, and then successively reported the occurrence and damage of verticillium wilt in states such as Mississippi, Arkansas, Texas, New Me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C05F11/08C05G3/00C05G3/04C05F17/00C12R1/125C05G3/80
CPCY02W30/40
Inventor 沈其荣罗佳冉伟杨兴明胡江黄启为
Owner 河南省全元农业科技有限公司
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