A self-aligned trench DMOS transistor structure of the present invention comprises a self-aligned source structure and a self-aligned trench gate structure, in which the self-aligned source structure comprises a p-base diffusion region, a self-aligned n+ source diffusion ring, a self-aligned p+ contact diffusion region, and a self-aligned source contact window; the self-aligned trench gate structure comprises a self-aligned silicided conductive gate structure, a self-aligned polycided conductive gate structure or a self-aligned polycided trenched conductive gate structure. The self-aligned trench DMOS transistor structure as described is fabricated by using only one masking photoresist step and can be easily scaled down to obtain a high-density trench DMOS power transistor with ultra low on-resistance, low gate-interconnection parasitic resistance, and high device ruggedness.