The invention discloses a
screening method of a non-invasive gastric
cancer saliva biomarker. The
screening method specifically comprises the following steps:
protein sample preparation,
protein quantification,
proteolysis and freeze-
drying, labeling a sample by iTRAQ, C18 desalting, liquid-phase separation and
mass spectrum identification, and
data analysis; the GO function enrichment including
biology progress, the molecule function and the
cell component is performed on the screened differential
protein by using the Panther Classification
System in the
data analysis; and then the protein mutual
effect analysis is performed on the differential protein by using the
STRING analysis software; and then the KEGG
signal path analysis is performed on the differential protein. Through the
screening method disclosed by the invention, the specificity protein marker in the gastric
cancer can be discovered through a fast and reliable
proteomics technical program, thereby establishing a non-invasive, simple and convenient, fast and practical detection evaluation measure for the early diagnosis, the postoperative recurrence,
metastasis, and prognosis observation of the gastric
cancer.