Screening method of non-invasive gastric cancer saliva biomarker

Abstract

The invention discloses a screening method of a non-invasive gastric cancer saliva biomarker. The screening method specifically comprises the following steps: protein sample preparation, protein quantification, proteolysis and freeze-drying, labeling a sample by iTRAQ, C18 desalting, liquid-phase separation and mass spectrum identification, and data analysis; the GO function enrichment including biology progress, the molecule function and the cell component is performed on the screened differential protein by using the Panther Classification System in the data analysis; and then the protein mutual effect analysis is performed on the differential protein by using the STRING analysis software; and then the KEGG signal path analysis is performed on the differential protein. Through the screening method disclosed by the invention, the specificity protein marker in the gastric cancer can be discovered through a fast and reliable proteomics technical program, thereby establishing a non-invasive, simple and convenient, fast and practical detection evaluation measure for the early diagnosis, the postoperative recurrence, metastasis, and prognosis observation of the gastric cancer.

Description

technical field [0001] The invention relates to a screening method for biomarkers, in particular to a screening method for non-invasive gastric cancer salivary biomarkers. Background technique [0002] Gastric cancer is one of the most common malignant gastrointestinal tumors worldwide. The onset of gastric cancer is hidden, and the early symptoms and signs are not obvious, so it needs to rely on auxiliary examinations for diagnosis. At present, routine gastroscopy and biopsy of pathological tissue obtained under the microscope are recognized as the gold standard for the diagnosis of gastric cancer, but it is still difficult to carry out large-scale census in my country. Extensive screening programs of asymptomatic individuals by gastric fluoroscopy have increased the number of early gastric cancer cases detected, increasing the overall 5-year survival rate to over 50%. It has good sensitivity and specificity, however, cost-effectiveness studies indicate that gastric fluor...

AI Technical Summary

Problems solved by technology

At present, Japan has used PG as a tumor marker for early diagnosis and screening of gastric cancer, but there is no large-scale evidence to support it in my country, and the level of PG in serum is affected by various factors (regional differences, dietary structure, race, etc.) , and there may be different diagnostic criteria for different detection groups, therefore, the value of screening gastric cancer by detecting serum PG levels in China still needs to be confirmed by the results of long-term longitudinal studies with large samples

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