The present invention relates to the technical field of earphone acoustic structure, in particular to an ANC earphone acoustic test system, which includes an audio system, a test sound card, a test microphone, a converter, and a console. The audio system includes a test speaker, and the test speaker includes an analog front shell and a simulated rear case, the simulated front case is provided with a front chamber, the simulated front case is also provided with a middle chamber, a channel and a leakage hole, and a test space for accommodating the measured resonance damping speaker box is arranged in the middle chamber , the inner wall of the middle cavity is provided with a mounting hole, one end of the leakage hole is connected to the front cavity, and the mounting hole is provided with an elastic flat pressure tube body for supporting and connected with the inside of the resonance damping speaker box, and the flat pressure tube body One end is plugged into the mounting hole. An ANC earphone acoustic testing system of the present invention can be used to test a resonance damping speaker box with a special structure, is easy to install and replace the measured resonance damping speaker box, and simulates the actual earphone assembly environment of the speaker.