39 results about "M13 bacteriophage" patented technology

An inorganic nanowire having an organic scaffold substantially removed from the inorganic nanowire, the inorganic nanowire consisting essentially of fused inorganic nanoparticles substantially free of the organic scaffold, and methods of making same. For example, a virus-based scaffold for the synthesis of single crystal ZnS, CdS and free-standing L10 CoPt and FePt nanowires can be used, with the means of modifying substrate specificity through standard biological methods. Peptides can be selected through an evolutionary screening process that exhibit control of composition, size, and phase during nanoparticle nucleation have been expressed on the highly ordered filamentous capsid of the M13 bacteriophage. The incorporation of specific, nucleating peptides into the generic scaffold of the M13 coat structure can provide a viable template for the directed synthesis of a variety of materials including semiconducting and magnetic materials. Removal of the viral template via annealing can promote oriented aggregation-based crystal growth, forming individual crystalline nanowires. The unique ability to interchange substrate specific peptides into the linear self-assembled filamentous construct of the M13 virus introduces a material tunability not seen in previous synthetic routes. Therefore, this system provides a genetic tool kit for growing and organizing nanowires from various materials including semiconducting and magnetic materials.

The invention provides an electrode composite material which comprises a M13 bacteriophage and Mn3O4. The electrode composite material has a good nano structure and a good electrochemical performance, and can be used as a negative electrode material of a lithium ion battery; moreover, a preparation method of the electrode composite material is simple and feasible, and can be implemented at low temperature. The invention also provides the preparation method of an electrode composite material, and a negative electrode and a battery which comprise the electrode composite material.

The invention relates to a compositions and methods for generating and using pIX phage display libraries for producing non-antibody peptide or protein proteins or peptides using engineered hybrid phage vectors derived from pIX of M 13 phage.

Phakopsora pachyrhizi, the fungal pathogen that causes Asian soybean rust, has the potential to cause significant losses in soybean yield in many production regions of the U.S. To assist the development of new modes of soybean resistance to fungal infection, peptides were identified from combinatorial phage-display peptide libraries which inhibit germ tube growth from urediniospores of P. pachyrhizi. Two peptides, Sp2 and Sp39, were identified that inhibit germ tube development when displayed as fusions with the coat protein of M13 phage or as fusions with maize cytokinin oxidase / dehydrogenase (ZmCKX1). These peptides may be used in vitro to help control fungal infection. These peptides may also be expressed as transgenes in a plant to help the resulting transgenic plant defend against the fungi.

The invention relates to a compositions and methods for generating and using pIX phage display libraries for producing non-antibody peptide or protein proteins or peptides using engineered hybrid phage vectors derived from pIX of M 13 phage.

The invention discloses a bacteriophage electrochemical luminescence signal amplification probe and a preparation method and application thereof. The bacteriophage electrochemical luminescence signal amplification probe comprises M13 bacteriophage, streptavidin expressed in the M13 bacteriophage in a fusion manner and tris-(bipyridyl) ruthenium which is covalently connected to the M13 bacteriophage. According to the bacteriophage electrochemical luminescence signal amplification probe, the M13 bacteriophage is used as a connecting skeletion for amplifying tris-(bipyridyl) ruthenium signals, and has the advantages of stable system, difficulty in gathering, easiness in storage and the like. The prepared signal amplification probe has the advantages of simplicity, controllability, high signal amplification efficiency and the like, and can be used for quantitative detection of nucleic acid or antigen and the like.

The invention discloses a linear neutralizing epitope polypeptide of fowl reticuloendotheliosis virus gp90 protein and application thereof. According to the invention, a random 12-peptide library (Ph.D.-12TM) of M13 bacteriophage is used for performing biological selection on monoclonal antibody A9E8 with neutralizing activity of the fowl reticuloendotheliosis gp90 protein for three times, so as to obtain 8 bacteriophage-positive clones, which can perform specific combination reaction with A9E8; and then fusion 12 peptides of the positive clones are subjected to sequencing and sequence comparison to analyze that the epitope peptide motif identified by A9E8 is SVQYHPL, and sequence comparison discovers that the motif is highly conserved in the fowl reticuloendotheliosis virus group, so that the epitope is inferred to be the fowl reticuloendotheliosis virus group specific epitope. According to the invention, theoretical basis is provided for development of gene engineering diagnosis reagents and design of polypeptide vaccines based on epitope.

The invention provides a microelectrolysis system as well as a preparation method and an application thereof. The microelectrolysis system comprises bacteriophage M13, first metal and second metal, wherein the first metal and the second metal are distributed on the surface of the bacteriophage M13 and have a potential difference. The first metal and the second metal have the potential difference, so that the microelectrolysis system can be formed effectively and can be used for performing reductive treatment on organic pollutants or heavy metal ions in sewage, the reduction rate is high, required time is short, treatment can be performed at normal temperature and normal pressure, and the microelectrolysis system does not need a noble metal catalyst and is lower in cost.

The invention provides an electrode composite material which comprises a M13 bacteriophage and Mn3O4. The electrode composite material has a good nano structure and a good electrochemical performance, and can be used as a negative electrode material of a lithium ion battery; moreover, a preparation method of the electrode composite material is simple and feasible, and can be implemented at low temperature. The invention also provides the preparation method of an electrode composite material, and a negative electrode and a battery which comprise the electrode composite material.

The invention discloses a preparation method of a RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material, and relates to a preparation method of a hemostatic material. The invention aims to solve the problems that in the prior art, after the modification of conventional oxidized regenerated cellulose, the hemostatic time is slightly prolonged, while the mechanical strength and biological absorbability of oxidized regenerated cellulose are both reduced. The preparation method comprises the following steps: step one, activating oxidized regenerated cellulose; step two, soaking the activated oxidized regenerated cellulose in RGD-M13 bacteriophage suspension so as to obtain the RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material. The prepared RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material can be used to stop bleeding, and hemostatic time is shortened by 10.4 to 32.9%. The provided prepared method can obtain a RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material.

The invention discloses a preparation method of an RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material, and relates to a preparation method of a haemostatic material. The invention aims at solving the problems of an existing oxidized regenerated cellulose modified haemostatic material that a haemostatic time is lightly improved, and the mechanical strength and bio-absorbing property of the oxidized regenerated cellulose are decreased. The method comprises the following steps: I. self-assembly of polylysine; II. self-assembly of an RGD-M13 phage; and III. cleaning and drying, so that the RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material is obtained. The RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material prepared by the invention, when applied to haemostasis, can shorten the haemostatic time by 25.17%-41.95%. The invention provides the RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material.

The present invention provides a novel peptide with high specificity for recognition of ovarian cancer. The peptide is selected using M13 phage display library and epithelial-enrich-conjugated magnetic beads. Only a small amount of the peptide is required to establish over 80% binding activity to ovarian cancer comparing to low binding activities to other cancers, showing high specificity for ovarian cancer. Thus the peptide of the present invention can be effective in early detection of ovarian cancer.

The invention provides a method for rapidly screening a bacterial quorum sensing inhibitor (QSI) by utilizing a luminescence method. The method is characterized by infecting escherichia coli ER2738 carrying pSB536 by utilizing the characteristic that a phage M13 does not lyse a host bacterium and can be secreted outside cells and then screening a bacterial clone which is blue under visible light and does not emit or emits weak biological fluorescent light under exciting light in a plate to which a C4-AHL molecule, tetracycline, ampicillin and X-gal / IPTG are added; then amplifying the phage, carrying out streak culture in the plate to which the substances are added, verifying the functions of the QSI by a method of inhibiting bioluminescence and finally obtaining specific QSI polypeptide sequences through sequencing. The method is simple in steps, and multiple QSI candidate polypeptides can be obtained only within three days to about one weak, so that the method has very obvious advantages.

The invention discloses a novel micro-environment biological macromolecule oscillator. The oscillator relies on DNA origami, uses single-stranded-base genes of a M13 phage as a skeleton, uses more than 100 kinds of single-stranded oligonucleotide primers to fold skeleton nucleic acid into a stable trigeminy clip structure, and combines with the affinity of aptamer on biological macromolecules, the closing and opening of clips can dynamically capture and release free proteins to achieve a regulatory function of the concentration of micro-environment biological macromolecules. The opening and closing state of an outer clip and a middle clip are always different. When the oscillator adjusts the concentration of a pair of macromolecules with antagonistic functions, the metabolic reaction rate and the reaction direction can be controlled. The structure of a DNA device is observed by an atomic force microscope, and the ability to regulate and control protein molecules is demonstrated by experiments such as in vitro blood coagulation.