32 results about "Fritillaria taipaiensis" patented technology

The invention belongs to the field of planting of crops and particularly relates to a method for planting fritillaria taipaiensis. The method comprises the steps of land selection, land preparation, dibble seeding, shading shed building, topdressing, weeding, disease and insect pest prevention and control and harvesting. According to the method, a dibble seeding method is adopted during sowing, thus, the consumption of seeds is low, the germinating rate is high, the survival rate of plants is high, the planting cost is low, the production cost is reduced effectively, and the production yield is increased; a special substrate is used for covering after sowing, so that the embryo differentiation of seeds is better promoted; meanwhile, the amount of fertilizer application and various cultivating parameters are strictly controlled during planting, an organic fertilizer and soil of planting areas are disinfected with carbendazim, a chlordane emulsifiable concentrate is added, and thiophanate-methyl is sprayed to the planting areas during the growth of the fritillaria taipaiensis, so that a rust disease can be prevented, wireworms and the like can be prevented from damaging plants, and diseases and insect pests of bulbs of the fritillaria taipaiensis are reduced; and calcium superphosphate or potassium dihydrogen phosphate is additionally applied in flowering and fruiting periods of the fritillaria taipaiensis, so that the yield and quality of the seeds and commodity bulbs are improved.

The invention relates to a Taipei fritillary bulb planting method. The Taipei fritillary bulb planting method comprises selecting and leveling sandy soil at the altitude of 2000 to 5000 m, making beds, planting, disinfecting, timely covering soil after the Taipei fritillary bulbs damp off and starting digging four years after seeding. The Taipei fritillary bulb planting method has the advantages of reducing diseases and insect pests of the Taipei fritillary bulbs; avoiding rust diseases and avoiding plants from being damaged by wireworms due to the fact that the organic fertilizer and the soil in the planting region are sterilized through carbendazim and added chlordane emulsifiable concentrate and 50 % of the thiophanate 1000 times liquid is sprayed in the planting region during the growing process of the Taipei fritillary bulbs; improving yield and quality of seed and commodity bulbs.

The invention discloses a greenhouse breeding method for Fritillaria taipaiensis. The greenhouse breeding method for Fritillaria taipaiensis realizes wild shifting breeding through introduction of Fritillaria taipaiensis applicable to growing at a low altitude of 1650 to 2800 meters by adopting an artificial greenhouse cultivation technique. According to the invention, wild shifting breeding realized through introduction of Fritillaria taipaiensis applicable to growing at the low altitude (1650 to 2800 meters) by adopting the artificial greenhouse cultivation technique is an effective means to expand the medicinal resource of Fritillaria taipaiensis, and solves the problem of scale cultivation at low altitudes.

The invention belongs to traditional Chinese medicine appraisal field, it is priming for appraising the Chinese Fritillaria taipaiensis. The priming DNA sequence is: the priming 1:5'-CTAAATAGGCGGGCGAGCTA ATC-3'; the priming 2:5'-TATTAGCATT AATCGATCGAATTC-3'. The appraising method is: extracts the total DNA of medicinal materials; uses a couple of priming AS, AS-1 to expand the DNA in order to check its quality, if there is positive expansion, the model DNA is eligible; uses the appraising priming to expand it; uses electrophoresis to check the result, if gets the positive expansion, it is Chinese Fritillaria taipaiensis, if not, it isn't zhebeimu.

The invention relates to the technical field of accessory devices for fritillaria taipaiensis processing equipment, particularly to a fritillaria taipaiensis sun-drying device. The fritillaria taipaiensis sun-drying device can improve usage convenience of existing sun-drying devices through arranging an automatic fritillaria taipaiensis flattening device, and can improve fritillaria taipaiensis sun-drying sufficiency of the existing sun-drying devices through arranging a turning device. The fritillaria taipaiensis sun-drying device comprises a sun-drying table, a plurality of sets of left supporting legs and a plurality of sets of right supporting legs; further comprises a location limiting plate, a sliding block, a fixing plate, a motor, a fixing ring, two sets of hanger rods, a transmission shaft, a power gear, a gear strip, a fixed bearing, a driving plate, an upper rail rod, a lower rail rod, a clamping sleeve and a scattering device, wherein the scattering device is provided witha containing box, a transmission pipe and a separating box; and further comprises two sets of limiting plates, a pushing shaft, two sets of clamping shafts, a lifting rod, a power clamping rod, a chain, a driven chain wheel, a driving chain wheel, a driven hanging rod, a driven bearing, a driven rotating shaft, a driving hanging rod, a driving bearing, a driving rotating shaft, a supporting frameand a rotating rod.

A fritillaria taipaiensis processing method is disclosed. The method is characterized in that the method includes (1) a picking and cleaning step, (2) a low-temperature cold-wind drying step, (3) an ultralow-temperature rapid freeze drying step, (4) a low-temperature hot wind drying step, and (5) an ultrahigh-pressure low-temperature sterilization step. The whole process from the cleaning step, the drying step to production of a finished product is performed at low temperatures, thus avoiding medicine material deformation and deterioration problems caused by excessively fast dehydration of a traditional Chinese medicine in high-temperature high-pressure environments, protecting effective components from being destroyed, and effectively maintaining bioactive compounds and nutrients in the traditional Chinese medicine. A product of the method is free of fumigation with sulfur so that the product meets a food standard on the sulfur dioxide content. A combined drying manner is adopted forthe fritillaria taipaiensis, and therefore the bioactive compounds and nutrients in the traditional Chinese medicine are maintained to the utmost, a problem that decoction pieces are liable to be mushy in decoction is solved, drying time is shortened, and better drying effects can be achieved.

The invention relates to a method for planting Fritillaria taipaiensis. The method includes the following steps: seed treatment is performed; a planting zone is established in a sandy soil zone with the altitude of 2000-4000 m, the planting land is flatted in September-October, beds are made, and a base fertilizer is applied on the beds; soil disinfection is performed on the base fertile and the planting zone, and then sowing and soil covering are performed, young seedlings of the Fritillaria taipaiensis are divided into different beds and shaded in the first year and the second year, wherein the canopy density of the first year is 60%, and the canopy density of the second year is 40%; in middle and late March every year, before the Fritillaria taipaiensis germinates, covering articles on surfaces of the beds are removed, and fumigated soil or a disinfected matured organic fertilizer of 2000-3000 kilograms / 667 m2 are applied; in June-July, superphosphate of 40-60 kilograms or monopotassium phosphate of 40-60 kilograms is applied once during a flower and fruit period of the Fritillaria taipaiensis; weeding is performed timely during planting; and product Fritillaria taipaiensis can be harvested in the fourth year after sowing. The method for planting the Fritillaria taipaiensis is high in survival rate and yield of plant.

The invention discloses a planting method of Fritillaria taipaiensis in a greenhouse. The planting method comprises the following steps of building a greenhouse, preparing a substrate, selecting and treating seeds, sowing, cultivating and managing, and preventing and controlling pests. The method utilizes a compound microbial agent I to disinfect the substrate to prevent the seeds from infecting virus and also utilizes the compound microbial agent I during the later period of growth to prevent the infection of root pathogenic fungi, prevent or directly cure a variety of diseases, promote the growth of roots and the healthy growth of Fritillaria taipaiensis, and increase the yield; temperature control in the planting method is conducive to the accumulation of medicinal ingredients of Fritillaria taipaiensis so as to improve the quality of Fritillaria taipaiensis; as fungi and hormones are applied to pest control, the planting method is non-toxic and non-polluting.

The invention relates to a Chinese medicinal preparation, in particular to an oral Chinese medicament for treating exterior cold and interior heat type chronic bronchitis. The oral Chinese medicament is prepared from the following raw materials: 9 to 12 grams of ash bark, 9 to 12 grams of plantain seed, 13 to 15 grams of lilium pumilum, 13 to 15 grams of fritillaria taipaiensis, and 18 to 20 grams of nightshade. The oral Chinese medicament has the characteristics of low cost, simplicity in preparation, low toxic or side effect and good curative effect.

The invention belongs to the technical field of traditional Chinese medicine powder grinding and preparation, and discloses a preparation method of superfine freeze-dried powder of Fritillaria taibai. The invention uses Fritillaria taibai with mature cultivation technology and high yield as raw material, which is cleaned and pulverized by ultrasonic waves, and mechanically milled. Slurry, centrifugal liquid separation, high-pressure micro-jet homogenization, cold sterilization, and finally freeze-drying or spray-drying, and sieving to obtain the target product Taibai Fritillaria superfine freeze-dried powder. The preparation method of Taibai Fritillaria ultrafine freeze-dried powder of the present invention is helpful to retain various alkaloids in Taibai Fritillaria, the preparation method is simple, time-consuming, suitable for industrial production, and the total alkaloids in Taibai Fritillaria is retained to the greatest extent. Alkaloid components and active components; at the same time, the present invention adopts high-pressure cold sterilization, strong bactericidal power, no loss of drug efficacy, no toxins and unknown substances produced by the drug after sterilization, convenient, economical and effective operation, and the prepared Taibai Fritillaria ultramicro Freeze-dried powder is a nano-level product, which is convenient for its subsequent direct use and further processing.

The invention discloses a traditional Chinese medicine for treating pemphigus erythematosus. The traditional Chinese medicine comprises the following Chinese herbal medicines: Jackinthepulpit tuber, herb of tufted bracket plant, flower of Japanese kerria, marshy betony, herb of hispid arthraxon, Mongolian peach, bulb of Fritillaria taipaiensis P.Y.Li, root of giant timber bamboo, snakegourd seed, herb of Peking spleenwort, Chinese usnea, root of younghusband jerusalemsage, fruit of lingnan artocarpus, juice of Japanese white birch., Artemisia halodendron Turcz., root of Japanese avens, nacre, chlorite schist and rhizoma pinelliae pedatisectae. The traditional Chinese medicine takes the pure natural Chinese herbal medicines as raw materials, treats both symptoms and root causes, has definite curative effects, does not have toxic or side effects and enables the disease not easy to recur.

The invention discloses a traditional Chinese medicine enema for treating acute upper respiratory infections of children. The Chinese medicine believes that most acute upper respiratory tract infection causative agents are viruses, and also can be complicated by bacterial infection; and aetiological agents are mainly tender viscera, thin skin, weakened external health, invasion of the wind evil to cause cold. The enema is prepared by the following traditional Chinese medicines: wrightia leaf, ilex pubescens, ephedra, schizonepeta, passiflora foetida, chrysanthemum, radix bupleuri, subprostrate sophora, fritillaria taipaiensis, nandina, platycodon grandiflorum, orange peel and mangnolia officinalis, which have the functions of dispelling wind, relieving exterior syndrome, freeing lung, relieving asthma, clearing heat, removing toxicity, regulating vital energy and tonifying spleen. Through clinical test, the total effective rate is 95.4 percent, which is obviously superior to a controlgroup, the treating cost is low, the drug administration is convenient, and the enema has no side effect, and is worthy of being widely popularized and applied in clinic.

The invention discloses a seedling growing and planting device for fritillaria taipaiensis. The device comprises a stirring unit, a shaking unit, a broadcast sowing unit, a water spraying unit, a reciprocating unit and a switch unit. The stirring unit comprises a first discharging port and a second discharging port and is located over the shaking unit and the broadcast sowing unit, and the first discharging port and the second discharging port correspond to feeding ports of the shaking unit and the broadcast sowing unit respectively. Discharging ports of the shaking unit, the broadcast sowing unit and the water spraying unit are all located above the reciprocating unit and correspond to a feeding port of the reciprocating unit, and the switch unit is further arranged between the first discharging port and the second discharging port of the stirring unit and the feeding ports of the shaking unit and the broadcast sowing unit. The reciprocating unit selectively communicates the first discharging port and the second discharging port of the stirring unit with the feed ports of the shaking unit and the broadcast sowing unit through the switch unit in the reciprocating movement process.

The invention discloses a gentiana pubigera Marq health tea. The gentiana pubigera Marq health tea is prepared by mixing gentiana pubigera Marq, galinsoga parviflora Cav, lentil and fritillaria taipaiensis according to a certain weight ratio. The health tea has the efficacies of reducing fever and reducing heat, diminishing inflammation and disintoxicating, clearing throat and relieving pain, moistening lung by reducing phlegm, and suppressing cough, is low in cost, good in effect, reasonable in formula, simple in preparation, easy to store, and convenient to drink, is suitable for being drunk by people for four seasons, can realize the effect of treatment of diseased people, and health protection of healthy people, is suitable for being drunk by people suffering from throat irritation, tonsils inflammation, acute gastroenteritis, lung heat cough, bronchitis, uncomplete sputum and the like, has an excellent effect after being drunk, and is an ideal and excellent health drink.

The invention relates to a method for establishing a regeneration system of bulb of fritillaria taipaiensis P. Y. Li with seeds as an explant, and relates to the field of plant regeneration.The methodcomprises the following steps: S1, obtaining the seeds of the fritillaria taipaiensis P. Y. Li; S2, pretreating the seeds of the fritillaria taipaiensis P. Y. Li to obtain pretreated seeds; S3, sterilizing the pretreated seeds to obtain sterilized seeds; S4, performing seed callus induction treatment on the sterilized seeds to obtain loose granular callus; S5, putting the loose granular callus ina culture medium added with 6-BA and 2,4-D for successive proliferation for three generations to obtain a large amount of the loose granular callus, wherein one generation needs 30-40 days. The method for establishing the regeneration system of the bulb of the fritillaria taipaiensis P. Y. Li with the seeds as the explant solves the technical problem of how to improve the emergence rate of high-quality fritillaria taipaiensis P. Y. Li, and is applicable to the cultivation of the seedlings of the fritillaria taipaiensis P. Y. Li.

The invention discloses a traditional Chinese medicine enema for treating acute upper respiratory infections of children. The Chinese medicine believes that most acute upper respiratory tract infection causative agents are viruses, and also can be complicated by bacterial infection; and aetiological agents are mainly tender viscera, thin skin, weakened external health, invasion of the wind evil to cause cold. The enema is prepared by the following traditional Chinese medicines: wrightia leaf, ilex pubescens, ephedra, schizonepeta, passiflora foetida, chrysanthemum, radix bupleuri, subprostrate sophora, fritillaria taipaiensis, nandina, platycodon grandiflorum, orange peel and mangnolia officinalis, which have the functions of dispelling wind, relieving exterior syndrome, freeing lung, relieving asthma, clearing heat, removing toxicity, regulating vital energy and tonifying spleen. Through clinical test, the total effective rate is 95.4 percent, which is obviously superior to a controlgroup, the treating cost is low, the drug administration is convenient, and the enema has no side effect, and is worthy of being widely popularized and applied in clinic.

The invention relates to the technical field of auxiliary devices of fritillaria taipaiensis planting equipment, in particular to fritillaria taipaiensis fruit picking equipment. The fritillaria taipaiensis fruit picking equipment can improve the picking efficiency of existing fritillaria taipaiensis fruit picking equipment by removing soil on the surface of fritillaria taipaiensis in batches, andcorrespondingly the practicability is improved. The use convenience of the existing fritillaria taipaiensis fruit picking equipment is improved by loading the picked fritillaria taipaiensis fruits into a woven bag at one time, so that the use limitation is reduced. The fritillaria taipaiensis fruit picking equipment comprises a spade, the woven bag, a screening box, four groups of supporting legs, a vibrating filter plate, a driving rod, a limiting plate, an adjusting rod, a fixing bearing, a driven wheel, a power shaft, a driving wheel, a belt, a motor and a mounting plate, wherein a workingcavity is formed in the screening box. The equipment also comprises a storage box, a baffle, two groups of rotating sheets, a supporting plate, a lifting rod, a chuck, a control plate and a pushing spring, wherein the right end of the storage box is connected with the lower side of the middle of the left end of the screening box, and a containing cavity is formed in the storage box.

The invention discloses a method for planting bulbus fritilariae. The method comprises the following steps: leveling off sand soil at the altitude of 1,500 to 5,000m above the sea level to make furrows; then carrying out planting sterilization; covering soil on fritillaria taipaiensis in time after seedling falling; and digging bulbus fritilariae in the third year after sowing. The method can improve the biomass in the growing period of fritillaria taipaiensis, reduce the damage of fritillaria taipaiensis bulbs caused by diseases and insects, and improve the yield per unit area of fritillariataipaiensis. The method solves the bottleneck problem of the acclimatized planting of wild fritillaria taipaiensis. The method has simple steps and low cost, can simultaneously and effectively protect the environment, and can prevent soil and water loss; in addition, the method is more easily accepted by planting farmers, and is favorable for popularization.

The invention provides an Indel marker primer, method and application for quickly identifying Fritillaria taibai, and belongs to the technical field of identification of source varieties of Chinese medicinal materials. The present invention finds for the first time that the PCR method based on the Indel marker primer can specifically identify Taibai Fritillaria, and only Taibai Fritillaria shows positive results at the 302bp position, while other Fritillaria species show negative results. It can be used for the specific molecular identification of the original plants and medicinal materials of Taibai Fritillaria. The present invention has the following advantages: 1. good specificity, only Fritillaria Taibai shows a positive result at the 302bp position; 2. high sensitivity, the minimum detection concentration (DNA) of PCR is 0.239ng / μL; 3. few steps and short detection cycle: The operation time is only 2‑3 hours; ④ Good reproducibility: more than 10 biological repetitions have been carried out, and the reproducibility reaches 100%.

The invention discloses a fritillaria taipaiensis crude polysaccharide yogurt. A preparation method comprises the following steps: (1) grinding a dry fritillaria taipaiensis product into powder and sieving; (2) degreasing with petroleum ether and ethyl alcohol; (3) carrying out ultrasonic treatment; (4) removing protein; (5) homogenizing fresh cow milk; (6) preparing original lactic acid milk; (7) fermenting, namely mixing 160 parts of original lactic acid milk, 400 parts of fresh cow milk, 12-16 parts of white granulated sugar and 1-1.5 parts of fritillaria taipaiensis crude polysaccharide, stirring and mixing evenly, standing and fermenting at 42-43 DEG C for 7-8 hours to obtain initial fritillaria taipaiensis crude polysaccharide yogurt; and (8) ageing at 4 DEG C for 24-36 hours, so as to obtain the fritillaria taipaiensis crude polysaccharide yogurt. The fritillaria taipaiensis crude polysaccharide yogurt prepared by the method is unique in flavor, abundant in nutrient, stable in quality, and beneficial to storage, and has good development prospect.

The invention relates to the field of biotechnology, in particular to a method for controlling endophytic bacteria contamination in tissue culture of Taibai Fritillaria. The control method of the present invention comprises: S1, explant selection and pretreatment: select the fresh bulb of Taibai Fritillaria, soak in washing powder solution, wipe, rinse, filter paper blot, standby; S2, explant disinfection treatment: pretreatment The final explants were submerged in mercuric chloride solution, stirred, placed on a clean bench, rinsed, dried, and set aside; S3, small bulb induction differentiation: split the explant scales obtained by S2 treatment, and cut them longitudinally into 2 ~4mm wide small pieces, inoculated on the culture medium added with penicillin and carbendazim; S4, subculture: subculture once every 20 days; S5 small bulb proliferation culture: transfer the healthy small bulbs obtained in S4 cultured on medium without the addition of any sterilant. The method for controlling endophytic bacteria pollution in tissue culture of Taibai Fritillaria of the present invention has a control rate of endophytic bacteria pollution of up to 96.5%.

The invention provides a tissue culture method using the bulbs of Fritillaria taibai as explants, which solves the difficulties in the supply of provenance in the artificial planting industry of Fritillaria taibai in the prior art, the long production cycle and high production cost, which lead to the artificial planting of Fritillaria taibai. Breeding technology cannot be vigorously developed and cannot meet the technical problems of market supply. In this method, 3-4-year-old bulbs of Fritillaria taibai are used as explants, and the tissue culture and rapid propagation of Fritillaria taibai is finally established through the steps of explant surface sterilization, primary culture, proliferation culture, bud induction culture, and rooting culture. system. The present invention can be widely used in intensive planting of Taibai Fritillaria in greenhouses, shortens the production cycle of Taibai Fritillaria medicinal materials, reduces production costs, better meets market needs, and can also be used to cultivate detoxified Fritillaria seedlings, reducing artificial high Diseases occur in the process of density planting, increase yield, reduce agricultural product input, and reduce costs.

The invention discloses a composition for controlling influenza, a preparation method and an application thereof. The composition comprises the following components by mass parts: 10-20 parts of Saposhnikovia divaricata, 10-20 parts of root of Pedicularis resupinala, 10-20 parts of Peliosanthes macrostegia, and 10-20 parts of Fritillaria taipaiensis. The invention is funded by a special forestry public-welfare object, namely Qing mountain forest herb resource protection, development and utilization technology research, of the State Forestry Bureau. Four characteristic Chinese herbal medicines in Taibai Mountain of Qing Mountain are preferably selected to compose the anti-flu traditional Chinese medicine prescription with the Saposhnikovia divaricata as the principal drug. pharmacodynamic animal tests, an acute toxicity test and a long-term toxicological test are carried out to evaluate pharmacodynamic efficacy and toxicological safety of the prescription, thereby ensuring that the prescription has a significant effect on treating influenza virus infection, especially the cold caused by H1N1 influenza virus, and is safe and nontoxic.

The invention relates to a method for introducing fritillaria taipaiensis seeds and prolonging the growth period of fritillaria taipaiensis, and belongs to the technical field of fritillaria taipaiensis cultivation. According to the growth condition of fritillaria taipaiensis, seeds are introduced from an original place, soaked and introduced in two stages, the dormancy period of fritillaria taipaiensis can be effectively shortened to about 90 days, direct sowing is conducted after dormancy is finished, and the growth period of the fritillaria taipaiensis is greatly prolonged; and high-quality seedlings are obtained by successfully introducing the fritillaria taipaiensis in different growth environments and altitudes and shortening the dormancy period of the seeds, the growth period is prolonged, and the yield and quality of commodity fritillaria taipaiensis are improved.

The invention discloses a method for accelerating after-ripening of fritillaria taipaiensis seeds. The method comprises the following steps that the fritillaria taipaiensis seeds, fine sand and vermiculite are laminated in a room after being uniformly mixed according to a ratio of 1 to 1 to 2, and the humidity of the mixed material is kept at 60%-70%; and lamination is continuously carried out 1-2 months, and when seed coats are expanded, germs grow to 2 / 3-3 / 4 of the length of endosperm and the germs are subjected to morphological after-ripening, low-temperature catalytic treatment is carried out until buds are about to grow and before the germs produce white buds, and sowing is started. According to the technical scheme, an improved seed treatment method is adopted, after-ripening and germ germination of the fritillaria taipaiensis seeds are accelerated by controlling the variety and proportional relation of a matrix, and combining a lamination container, lamination treatment conditions and a follow-up catalytic treatment means, the germination rate and the seedling uniformity are improved, time is saved, the production period is shortened, the efficiency is improved, the breeding limitation of fritillaria taipaiensis is broken through, and the method has high practical application significance.

The invention provides an Indel marker primer and method for rapidly identifying fritillaria taipaiensis and application, and belongs to the technical field of identification of source varieties of traditional Chinese medicinal materials. It is found for the first time that a PCR method with the Indel marker primer as the core can specifically identify fritillaria taipaiensis, only the fritillariataipaiensis shows a positive result at the 302bp position, and other fritillaria varieties show a negative result. The method can be used for specific molecular identification of fritillaria taipaiensis-based original plants and medicinal materials. The method has the following advantages: (1) the specificity is good, and only the fritillaria taipaiensis shows a positive result at a 302bp position; (2) the sensitivity is high, and the lowest detection concentration (DNA) of PCR is 0.239 ng / mu L; (3) the steps are few, the detection period is short, and the operation time is only 2-3 hours; and (4) the reproducibility is good: biological repetition is carried out for more than 10 times, and the reproducibility reaches 100%.