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36 results about "Cereal cyst nematode" patented technology
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Cereal cyst nematode (CCN) (syn. Cereal root eelworm and Oat cyst nematode) is a plant pest caused by Heterodera avenae, Heterodera bifenestra, Heterodera hordecalis, Heterodera latipons, and Heterodera gotland in the following hosts: Avena sativa, Hordeum vulgare, Secale cereale, Triticum aestivum, and X Triticosecale.
Nucleotide sequence of gene for resistance to Cereal cyst nematode, Heterodera avenae and application thereof
InactiveCN102234651AImprove resistance (tolerance)Plant peptidesFermentationBiotechnologyAegilops variabilis
The invention, relating to the field of plant genetic engineering, particularly relates to a nucleotide sequence of a gene from an allied specie Aegilops variabilis of wheat for resistance to Cereal cyst nematode, and an application on improving plant resistance to insects based on the gene through the method of biotechnology. The nucleotide sequence of the gene for resistance to Cereal cyst nematode disclosed in the invention is a complete encoding sequence of the gene for resistance to Cereal cyst nematode with the width of 3586bp, comprising 3 exons and 2 introns, and can code 934 amino acids, and is used in improving plant resistance.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Bacillus pumillus and application thereof in prevention and control of cereal cyst nematodes
ActiveCN103103155AWide adaptabilityMany mechanisms of actionBiocideBacteriaCystBULK ACTIVE INGREDIENT
Owner:CHINA AGRI UNIV
Heterodera avenae Ha-16674 protein, encoding gene and application thereof
The invention relates to heterodera avenae Ha-16674 protein, an encoding gene and application thereof. An amino acid sequence of the heterodera avenae Ha-16674 protein is shown as SEQ ID NO: 1, and a cDNA full-length nucleotide sequence of a gene for encoding the heterodera avenae Ha-16674 protein is shown as SEQ ID NO: 2. Experiments show that the Ha-16674 and Bax can inhibit blade cell death caused by the Bax when being jointly injected into tobacco leaves. Full-length overexpression of the Ha-16674 gene in arabidopsis thaliana can obtain stable genetic T3 generation transgene positive plants, and calprotectin Ha-16674 in heterodera avenae can reduce immune defence reaction of the arabidopsis thaliana to promote pesudomona syringae infection. The heterodera avenae Ha-16674 protein disclosed by the invention provides a new target Ha-16674 for nematode-resistance plant culture and has good theoretical guidance significance on culturing broad-spectrum heterodera-resistance crops.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Biocontrol strain 09B18 for preventing and controlling cereal cyst nematode of wheat, method for preparing biocontrol agent and application of biocontrol agent
The invention discloses a biocontrol strain 09B18 for preventing and controlling the cereal cyst nematode of wheat. The biocontrol strain 09B18 for preventing and controlling the cereal cyst nematode of wheat is bacillus cereus, and has the preservation number of CGMCCNO:8632. The biocontrol strain is a special biocontrol agent for the cereal cyst nematode of wheat; the biocontrol strain is a biological agent, so that a series of problems caused by the use of chemical pesticides are completely solved, and the pollution-free production of wheat crops is facilitated; the use of the other chemical pesticides can be avoided or reduced, so that the expenditures of farmers can be reduced, and the quality of grains is favorably improved; the biocontrol strain 09B18 is verified by prevention and control tests to have good prevention and control effects on the cereal cyst nematode of wheat, and the cyst reduction rate in indoor and field tests reaches 75.9 percent and 43.47 percent respectively; in addition, the biocontrol strain also has a yield-increasing effect, so that the income of the farmers can be increased.
Owner:HENAN AGRICULTURAL UNIVERSITY
Bacillus cereus for preventing and controlling plant nematodes and application thereof
ActiveCN108676761AHigh poisonous activityReduce in quantityBiocidePlant growth regulatorsPlant nematodeTriticeae
The invention discloses bacillus cereus for preventing and controlling plant nematodes and application thereof. The name of the bacillus cereus is MH61-63-03; the collection number is CGMCC No.15616.Experimental results prove that the bacillus cereus has the advantages that a fermenting filtrate of the bacillus cereus MH61-63-03 has higher toxic activity on second instar larvae of the plant nematodes; the number of nematodes in wheat roots treated by the fermenting filtrate MH61-63-03 is greatly reduced 7 days after the second instar larvae of the cereal cyst nematode are inoculated, and thereduction rate of insect is 26.82%; the number of sporocysts of each wheat plant treated by the fermenting filtrate MH61-63-03 is obviously reduced 65 days after the second instar larvae of the cerealcyst nematode are inoculated; the biological agent has good application and development prospect in the preventing and controlling of the plant nematodes.
Owner:CHINA AGRI UNIV
Cereal cyst nematode Ha34609 protein, encoding gene and application of encoding gene
The invention relates to a cereal cyst nematode Ha34609 protein, an encoding gene and application of the encoding gene. The amino acid sequence of the cereal cyst nematode Ha34609 protein is shown as SEQ ID NO: 1. The nucleotide sequence of the encoding gene is shown as SEQ ID NO: 2. After a silent Ha34609 gene is treated by adopting dsRNA (double-stranded ribonucleic acid), compared with eGFP (enhanced green fluorescent protein) dsRNA, the length and the width of a white female worm are remarkably reduced (t-test, confidence interval: 95%), which indicates that the gene plays an important role in parasitic and pathogenic processes of cereal cyst nematodes and can serve as a target gene of plant nematode resistance engineering. The cereal cyst nematode Ha34609 protein, the encoding gene and the application of the encoding gene have significant values in a study on a nosogenesis of the cyst nematodes and preparation of nematode resistance plants.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Coprinelllus radians and application thereof in preventing and curing cereal cyst nematode
The invention belongs to the technical field of plant disease biological control and relates to a Coprinelllus radians strain HMQAU090439N for preventing and curing the cereal cyst nematode. The preservation number of the Coprinelllus radians strain is CGMCC No.11814. The invention also discloses a microbial inoculum prepared by the above strain, and the application thereof in preventing and curing the cereal cyst nematode. According to the invention, an efficient microorganism for preventing and curing the cereal cyst nematode is provided. The Coprinelllus radians strain HMQAU090439N is good in parasitic and antagonism effect for the nematodes of the cereal cyst nematode. Meanwhile, the results of the pot experiment and the plot experiment show that, the Coprinelllus radians strain HMQAU090439N is good in control effect for the nematodes of the cereal cyst nematode. In addition, the microbial inoculum is safe to human bodies and livestock and is environment-friendly, thus having a good development and application prospect.
Owner:QINGDAO AGRI UNIV
Ha-62292 Protein, encoding gene and application of Heterodera avenae
The invention discloses Ha-62292 protein, encoding gene and application of Heterodera avenae. The invention provides a protein acquired from Heterodera avenae, and named Ha-62292 protein, having an amino acid sequence shown as in SEQ ID NO: 1, and a gene sequence for encoding the protein is shown as in SEQ ID NO: 2. Experiments show that the Ha-62292 gene plays an important role in infective parasitism of nematodes, and may act as a target gene for plant engineering for nematode resistance. The Ha-62292 protein, encoding gene and application of Heterodera avenae are of great significance to the study on pathogenesis of Heterodera avenae and the development of nematode control plants.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Biocontrol strain S07 for preventing and controlling cereal cyst nematode, method for preparing biocontrol fungicide and application of biocontrol fungicide
InactiveCN103805540AGood control effectCutting costsBiocidePlant growth regulatorsFungicideBiotechnology
The invention discloses a biocontrol strain S07 for preventing and controlling cereal cyst nematode. The biocontrol strain S07 for preventing and controlling cereal cyst nematode is an annular streptomycete (Streptomyces annulatus), the preservation number of which is CGMCCNO: 8630. The biocontrol strain disclosed by the invention is the biocontrol strain specially developed for cereal cyst nematode. As the biocontrol strain is a bio-preparation, a series of problems caused by use of chemical pesticides are fully avoided, so that the biocontrol strain is beneficial to pollution-free production of wheat crops. The use level of other chemical pesticides can be reduced or other chemical pesticides are not used by farmers, thus not only drawing the expenditures of the farmers, but also enhancing the quality of grains. Indoor and field controlling experiments verify that the S07 fungicide has a good preventing and controlling effect on cereal cyst nematode. Meanwhile, the fungicide has the function of increase production and can increase income for the farmers.
Owner:HENAN AGRICULTURAL UNIVERSITY
Lysinibacillus sp. for controlling plant nematodes and application thereof
ActiveCN108676762AHigh poisonous activityEnhanced inhibitory effectBiocideBacteriaPlant nematodeMeloidogyne incognita
The invention discloses lysine bacillus for controlling plant nematodes and application thereof. The name of the lysinibacillus sp. is BDGS-17-07; the collection number is CGMCC No.15617. Experimentalresults prove that the lysinibacillus sp. has the advantages that a fermenting liquid BDGS-17-07 has toxic activity on second instar larvae of cereal cyst nematodes, and eggs and second instar larvaeof meloidogyne incognita, and also has obvious inhibiting function on the hatching of egg particles and egg masses of the meloidogyne incognita; the fermenting liquid BDGS-17-07 also has good biological preventing effect; the preventing effect can reach 36% 60 days after the nematodes are inoculated by only applying 50mL of fermenting liquid BDGS-17-07; when the lysinibacillus sp. BDGS-17-07 (CGMCC No.15617) provided by the invention is used as the biological agent, the biological agent has good application and development prospect in the preventing and control of the plant nematodes.
Owner:CHINA AGRI UNIV
Oxalic acid penicillium NBC008 with nematocidal activity, and preparation method and application thereof
The invention relates to oxalic acid penicillium NBC008 with nematocidal activity, and a preparation method and application thereof. A fungus strain P.oxalicum NBC008 is separated and screened from a cereal cyst nematode colony in Ostfold county of Norway, and the collection number is CGMCC No. 12473. Through liquid fermentation and cultivation, fermented products have obvious toxic action on plant parasitic nematodes. Influences of strain fermentation liquid on second-instar larvas of soybean cyst nematodes and egg incubation are measured indoors. The fermentation liquid with different dilution concentrations has toxic action on the second-instar larvas of the soybean cyst nematodes, and has obvious inhibition effect on egg incubation. Fermentation stock solution can reduce the number of soybean sporocysts by 59.88%, and has good development and application prospect.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Nematicidal compound containing iprodione
The invention relates to a nematicidal compound which comprises the following active ingredients: a compound in the formula I and iprodione, wherein the weight ratio of the compound in the formula I and the iprodione is 30: 1-1: 20, and preferably, the weight ratio is 10: 1-5: 1. The active ingredients of the nematicidal compound has an obvious synergistic effect in prevention of various crop rootknot nematode diseases within a compound scope and is suitable for preventing various nematodes such as a wheat root knot nematode, a wheat cyst nematode, a citrus root knot nematode, a cucumber rootknot nematode, a tomato root knot nematode, a chili root knot nematode, a peanut root knot nematode, a soybean root knot nematode and the like. The nematicidal compound is good in prevention effect and long in lasting period, so that the nematicidal compound is worthy of promotion and application.
Owner:佛山市普尔玛农化有限公司
Heterodera avenae derived Ha-18764 protein and coding gene and application thereof
The invention discloses a heterodera avenae derived Ha-18764 protein and a coding gene and application thereof. The protein is obtained from heterodera avenae and named as the Ha-18764 protein and is (a1) or (a2), (a1) refers to a protein formed by an amino sequence shown as a sequence 1 in a sequence table, and (a2) refers to a (a1) derived protein associated with parasitism and / or pathopoiesia and / or development of heterodera avenae and is obtained by subjecting the (a1) to replacement and / or deficiency and / or addition of one or two amino acid residues. The coding gene of the Ha-18764 protein is named as a Ha-18764 gene which plays an important role in a nematode parasitism process and can serve as a target gene for plant nematode resistance engineering. The heterodera avenae derived Ha-18764 protein and the coding gene and application thereof have a significant value in heterodera avenae pathogenesis research and nematode-resistant plant preparation.
Owner:CHINA AGRI UNIV
RPA (recombinase polymerase amplification) primer, probe and kit for detecting heterodera avenae and application
ActiveCN114410802AHigh sensitivityStrong specificityMicrobiological testing/measurementAgainst vector-borne diseasesNucleotideMicrobiology
The invention discloses an RPA primer, a probe and a kit for detecting heterodera avenae and application, and belongs to the field of detection of plant parasitic nematodes. The RPA primer and the probe comprise a pair of specific primers HaRPA-F3 and HaRPA-R3 and a probe Ha-probe, the nucleotide sequences of the HaRPA-F3 and the HaRPA-R3 are as shown in SEQ ID NO: 1 to SEQ ID NO: 2, and the nucleotide sequence of the Ha-probe is as shown in SEQ ID NO: 3. The rapid visual detection method for the heterodera avenae is established by using the RPA primer and the probe, the sensitivity is high, the specificity is strong, and the whole detection process can be completed within 1 hour. Therefore, the RPA detection method disclosed by the invention is a simple, rapid, specific, high-sensitivity and intuitive method, and can be suitable for rapidly detecting the heterodera avenae in the field.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
A strain of Bacillus cereus for controlling plant nematodes and its application
ActiveCN108676761BHigh poisonous activityReduce in quantityBiocidePlant growth regulatorsBiotechnologyPlant nematode
The invention discloses a bacillus cereus for preventing and controlling plant nematodes and its application. The name of Bacillus cereus provided by the present invention is MH61‑63‑03, and the preservation number is CGMCC No.15616. The experimental results show that: the fermentation filtrate of Bacillus cereus MH61‑63‑03 has a high poisonous activity against the second instar larvae of plant parasitic nematodes; The number of wheat root nematodes treated with the filtrate decreased, and the population reduction rate was 26.82%. After 65 days of inoculation with the second instar larvae of cereal cyst nematodes, the average number of cysts per plant of wheat treated with MH61‑63‑03 fermentation filtrate also decreased. significantly reduced. The biological agent of the invention has good application and development prospects in the control of plant parasitic nematodes.
Owner:CHINA AGRI UNIV
Nematicidal preparation
The invention belongs to the field of pesticides, and particularly relates to a nematicidal preparation containing abamectin and T.longibrachiatum. The preparation is prepared from an abamectin solution and T.longibrachiatum bacteria liquid, so that an effect of toxically killing wheat cereal cyst nematode can be remarkably improved, a slow release effect is achieved, the lasting period of a pesticide can be prolonged, and the preparation is non-pollution and good in environmental compatibility, achieves low production cost, is capable of preventing nematode from easily becoming resistant to drugs, and has the effects of enlarging a prevention and cure spectrum and improving synergistic interaction, and the like.
Owner:GANSU AGRI UNIV
A strain of Bacillus lysinus for preventing and controlling plant nematodes and its application
ActiveCN108676762BHigh poisonous activityEnhanced inhibitory effectBiocideBacteriaBiotechnologyPlant nematode
The invention discloses a lysine bacillus for preventing and controlling plant nematodes and an application thereof. The name of Lysinibacillus sp. provided by the present invention is BDGS‑17‑07, and the preservation number is CGMCC No.15617. Experiments show that BDGS‑17‑07 fermented liquid has poisonous activity to the second instar larvae of graminearum cyst nematode and the eggs and second instar larvae of root-knot nematode incognita, and also has poisonous activity against egg grains and egg mass hatching of root-knot nematode incognita. The BDGS‑17‑07 fermentation broth also has a good biocontrol effect. Only 50mL of the BDGS‑17‑07 fermentation broth is applied, and the control effect can reach 36% when inoculated with nematodes for 60 days. The Bacillus lysinica BDGS‑17‑07 CGMCC No.15617 of the present invention has good application and development prospects as a biological agent in the prevention and treatment of plant parasitic nematodes.
Owner:CHINA AGRI UNIV
HaGLAND5 protein of cereal cyst nematode, encoding gene of protein and application
The invention discloses HaGLAND5 protein of cereal cyst nematode as well as an encoding gene and an application of protein. The protein is obtained from the cereal cyst nematode, is named as HaGLAND5protein and is protein formed by an amino acid sequence shown in a sequence 1 in a sequence list. The gene for encoding the HaGLAND5 protein is named as HaGLAND5 gene and also belongs to the protection range of the invention. The invention also protects an application of the HaGLAND5 protein. The application comprises regulation of parasitic ability of the cyst nematode; regulation of pathogenic ability of the cyst nematode; regulation of development of the cyst nematode. The invention also protects a method for cultivating a plant with improved resistance to the cyst nematode. The method comprises steps as follows: a substance for inhibiting HaGLAND5 gene expression is guided into a target plant, and the plant with improved resistance to the cyst nematode is obtained. The protein, the gene and the application have great value for research of the pathogenesis mechanism of the cereal cyst nematode and preparation of plants resisting nematode.
Owner:CHINA AGRI UNIV
Microdochium bolleyi F10 strain microbial inoculum as well as preparation method and application thereof
InactiveCN103525706BStrong parasitic effectSimple production technologyBiocideFungiSoil treatmentHigh pressure
The invention discloses a Microdochium bolleyi F10 strain microbial inoculum as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) activating a Microdochium bolleyi F10 strain on a PDA (potato dextrose agar) flat plate, then inoculating the strain to a PDA inclined plane for culture, adding sterilized deionized water to prepare a spore suspension, adding tween-20 to ensure that the final concentration of the tween-20 in the spore suspension is 0.025%, and adjusting to ensure that the concentration of the spore suspension reaches 1.0*10<7> / ml; (2) uniformly mixing dry culture material oatmeal with rice husks, adding water, and performing high-pressure steam sterilization; and (3) inoculating the prepared spore suspension to the culture material according to a ratio of 10ml / bag, culturing, airing and crushing to obtain the microbial inoculum. The Microdochium bolleyi F10 strain prepared by using the preparation method has a strong parasitic effect on cysts of heterodera avenae wollenwebers, the control effect of a bio-control microbial inoculum on cereal cyst nematode under a field cultivation condition reaches 41%, an application method of soil treatment is simple and saves labor force, and a biological pesticide is safe to human and livestock.
Owner:HUAZHONG AGRI UNIV
Compound medicament for preventing cereal cyst nematode and aphid of wheat
ActiveCN111328824AEnhanced inhibitory effectGood control effectBiocideNematocidesBiotechnologyNematode
The invention, which belongs to the field of pest control, particularly relates to a compound medicament for preventing cereal cyst nematode and aphid of wheat. The effective components of the compound medicament comprise imidacloprid and fluopyram in a mass ratio of 3: (0.4-6.7). According to the invention, the proportion of effective components in the compound medicament is improved, and finally, imidacloprid and fluopyram in a certain proportion are selected to be compounded as the effective components. Indoor sterile soil cultivation tests and field tests prove that the compound medicamentis free of phytotoxicity, high in budding rate and good in cyst nematode prevention and control effect; meanwhile, the compound medicament has a good inhibition rate and prevention and control effecton aphids.
Owner:HENAN AGRICULTURAL UNIVERSITY
A strain of Bacillus pumilus and its application in the control of cereal cyst nematode in wheat
Disclosed are a Bacillus pumilus strain and the use thereof in preventing and treating cereal cyst nematode in wheat, which strain is a Bacillus pumilus named B202 and deposited in CGMCC with a deposit number of CGMCC NO. 5838. Also disclosed is the use of the Bacillus pumilus strain B202 in the preparation of a biological protective preparation for cereal cyst nematode in wheat. The active ingredients of the biological protective preparation for cereal cyst nematode in wheat comprise at least one of the following (a) or (b) or (c) or (d): (a) a fermentation culture of the Bacillus pumilus strain B202; (b) the Bacillus pumilus strain B202; (c) spores of the Bacillus pumilus strain B202; and (d) a fermentation supernatant of the Bacillus pumilus strain B202. The Bacillus pumilus strain B202 has a stable prevention and treatment effect on cereal cyst nematode in wheat.
Owner:CHINA AGRI UNIV
Cereal cyst nematode ha-63744 protein, coding gene and its application
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Observation and determination method of effect of trichoderma T6 strain on plant parasitic nematode
InactiveCN110074064AMicrobiological testing/measurementAnimal husbandryNematodeMicroscopic observation
The invention belongs to the technical field of microorganism bio-control prevention, and particularly relates to an observation and determination method of effects of a trichoderma T6 strain on plantparasitic nematodes. The method is mainly characterized by comprising the following steps of adding sporocysts, second-age larvae and ova into a culture vessel; or adding second-age larvae, ova and trichoderma T6 strain spore suspension into a sterilized and disinfected 90-hole cell culture plate; adding fusarium oxysporum spore suspension and sterile water as reference respectively; performing microscopic observation and determination on the sporocyst parasitism and hatching, second-age larva death and ovum parasitism or hatching conditions. The method has the beneficial effects that throughmicroscopic observation and determination, the parasitism and lethal action mechanism of the trichoderma T6 strain on cereal cyst nematodes is analyzed and discussed, so that the theoretical basis isprovided for the preparation, development and reasonable application of novel and efficient microbial nematode killing agents.
Owner:GANSU AGRI UNIV
A kind of volatile goat grass phenylalanine ammonia lyase gene and its application
ActiveCN109762833BHigh resistance to cereal cyst nematodeImproved resistance to cereal cyst nematodesGenetic engineeringFermentationNucleic acid sequencingNucleic acid sequence
The present invention disclosed a kind of vulnerable goat alanine aminotramerase nucleic acid sequence and its application.The present invention is easy to change the goat phenylacease gene to encodes a phenylalanine, which has a high performance of anti -gray cysts, which has important reference and application value for the breeding of wheat anti -gravida cysts.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
4-benzylpiperidine amide compound and application thereof in controlling plant nematode disease
The invention discloses a 4-benzylpiperidine amide compound with a structural formula shown in the description. The compound has control effect on the plant nematode disease according to a meloidogyneincognita egg incubation inhibition test and an insecticidal activity test on different plant nematodes, wherein the nematodes are meloidogyne incognita, cereal cyst nematode and heterodera glycinesichinohe.
Owner:田元强
Variable aegilops tauschii phenylalanine ammonia-lyase gene and application thereof
ActiveCN109762833AHigh resistance to cereal cyst nematodeImproved resistance to cereal cyst nematodesFermentationVector-based foreign material introductionHigh resistanceBiotechnology
The invention discloses a variable aegilops tauschii phenylalanine ammonia-lyase nucleic acid sequence and application thereof. The variable aegilops tauschii phenylalanine ammonia-lyase gene nucleicacid sequence is shown as SEQ ID NO.1. The variable aegilops tauschii phenylalanine ammonia-lyase gene encodes a phenylalanine ammonia-lyase, has high resistance to cereal cyst nematodes, and has important reference and application values for breeding wheat resistance to cereal cyst nematodes.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Rapid detection method of heterodera avenae wollenweber LAMP and application of detection method
InactiveCN103045751BStrong specificityHigh sensitivityMicrobiological testing/measurementMicrobiologyDNA extraction
The invention relates to a rapid detection method of heterodera avenae wollenweber LAMP and application of the detection method. Five LAMP primers, namely HA5-F3, HA5-B3, HA5-FIP, HA5-BIP and HA5-LB are designed and screened according to a heterodera avenae wollenweber random amplified polymorphic dna (RAPD) sequence obtained by cloning; and an LAMP reaction system is configured and optimized. By performing DNA extraction, loop-mediated isothermal amplification, amplified product developing and observation on the heterodera avenae wollenweber, the heterodera avenae wollenweber can be rapidly detected. The detection method is high in specificity, low in cost and convenient to operate, and has high application values on rapid detection of the heterodera avenae wollenweber and early and field diagnosis of the heterodera avenae wollenweber diseases.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
4-(quinoline-4-methyl)piperidine amide compound and application thereof to plant nematodes diseases
The invention discloses a 4-(quinoline-4-methyl)piperidine amide compound, which has a structural formula shown in the description. The inhibition rate of the compound on the meloidogyne incognita eggincubation is 65.88; the insecticidal activity is realized on meloidogyne incognita second stage juveniles, cereal cyst nematode and heterodera glycines; the LD90 is respectively 67.5mu g / mL, 53.4mug / mL and 98.7mu g / mL. The poisoning and killing capability on the cereal cyst nematode is more obvious. The compound provided by the invention can be used for preventing and treating the plant nematode diseases.
Owner:田元强
HaHSP4 protein of cereal cyst nematode and coding gene and application thereof
The invention discloses a HaHSP4 protein of cereal cyst nematode and a coding gene and application thereof. The protein is obtained from the cereal cyst nematode and named the HaHSP4 protein which isthe protein consisting of the amino acid sequence shown in SEQ ID NO:1 in the Sequence Listing. A gene encoding the HaHSP4 protein is named the HaHSP4 gene, and also belongs to the scope of the invention. The invention also provides the application of the HaHSP4 protein. The application includes regulating the host tropism of the cyst nematode; regulating the parasitic capability of the cyst nematode; regulating the pathogenic capability of the cyst nematode; regulating the development of the cyst nematode. The invention also provides a method for cultivating a plant with improved resistance to the cyst nematode. The method comprises the steps of introducing a substance inhibiting the expression of the HaHSP4 protein into a target plant to obtain the plant with the improved resistance to the cyst nematode. The protein has great value for research on the pathogenic mechanism of the cyst nematode and the preparation of anti-nematode plants.
Owner:CHINA AGRI UNIV
A kind of caffeine extract of mutable goat grass and its extraction method and application
InactiveCN104522090BReduce dosageCause chemical pollutionBiocideNematocidesBio engineeringMeloidogyne incognita
The invention discloses a caffeine extract of goat grass vulgaris and its extraction method and application, belonging to the field of bioengineering. The specific steps of the extraction method of the caffeine extract of Goatweed vulgaris in the present invention are as follows: take the root of Goatweed vulgaris, wash it, cut it into slices, dry it in an oven at 80°C for 6 hours to remove moisture thoroughly, and then pulverize it with a pulverizer , after passing through a 60-mesh sieve, put 10 grams of pulverized goat grass root samples into a 50-milliliter extraction kettle, and use absolute ethanol as an entrainer. 2 30MPa in the extractor, extract at 60°C for 2h, in which CO 2 The flow rate is controlled at 1.5LPM to obtain the caffeine extract of mutable goat grass. The research of the present invention shows that >0.1% caffeine has a growth inhibitory effect on plant pathogenic nematodes, and the caffeine extract of goat grass vulgaris obtained in the present invention can be used to prepare insect-resistant insecticides for cereal cyst nematodes and root-knot nematodes incognita agent.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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