36 results about "Cereal cyst nematode" patented technology

The invention relates to heterodera avenae Ha-16674 protein, an encoding gene and application thereof. An amino acid sequence of the heterodera avenae Ha-16674 protein is shown as SEQ ID NO: 1, and a cDNA full-length nucleotide sequence of a gene for encoding the heterodera avenae Ha-16674 protein is shown as SEQ ID NO: 2. Experiments show that the Ha-16674 and Bax can inhibit blade cell death caused by the Bax when being jointly injected into tobacco leaves. Full-length overexpression of the Ha-16674 gene in arabidopsis thaliana can obtain stable genetic T3 generation transgene positive plants, and calprotectin Ha-16674 in heterodera avenae can reduce immune defence reaction of the arabidopsis thaliana to promote pesudomona syringae infection. The heterodera avenae Ha-16674 protein disclosed by the invention provides a new target Ha-16674 for nematode-resistance plant culture and has good theoretical guidance significance on culturing broad-spectrum heterodera-resistance crops.

The invention discloses a biocontrol strain 09B18 for preventing and controlling the cereal cyst nematode of wheat. The biocontrol strain 09B18 for preventing and controlling the cereal cyst nematode of wheat is bacillus cereus, and has the preservation number of CGMCCNO:8632. The biocontrol strain is a special biocontrol agent for the cereal cyst nematode of wheat; the biocontrol strain is a biological agent, so that a series of problems caused by the use of chemical pesticides are completely solved, and the pollution-free production of wheat crops is facilitated; the use of the other chemical pesticides can be avoided or reduced, so that the expenditures of farmers can be reduced, and the quality of grains is favorably improved; the biocontrol strain 09B18 is verified by prevention and control tests to have good prevention and control effects on the cereal cyst nematode of wheat, and the cyst reduction rate in indoor and field tests reaches 75.9 percent and 43.47 percent respectively; in addition, the biocontrol strain also has a yield-increasing effect, so that the income of the farmers can be increased.

The invention discloses bacillus cereus for preventing and controlling plant nematodes and application thereof. The name of the bacillus cereus is MH61-63-03; the collection number is CGMCC No.15616.Experimental results prove that the bacillus cereus has the advantages that a fermenting filtrate of the bacillus cereus MH61-63-03 has higher toxic activity on second instar larvae of the plant nematodes; the number of nematodes in wheat roots treated by the fermenting filtrate MH61-63-03 is greatly reduced 7 days after the second instar larvae of the cereal cyst nematode are inoculated, and thereduction rate of insect is 26.82%; the number of sporocysts of each wheat plant treated by the fermenting filtrate MH61-63-03 is obviously reduced 65 days after the second instar larvae of the cerealcyst nematode are inoculated; the biological agent has good application and development prospect in the preventing and controlling of the plant nematodes.

The invention relates to oxalic acid penicillium NBC008 with nematocidal activity, and a preparation method and application thereof. A fungus strain P.oxalicum NBC008 is separated and screened from a cereal cyst nematode colony in Ostfold county of Norway, and the collection number is CGMCC No. 12473. Through liquid fermentation and cultivation, fermented products have obvious toxic action on plant parasitic nematodes. Influences of strain fermentation liquid on second-instar larvas of soybean cyst nematodes and egg incubation are measured indoors. The fermentation liquid with different dilution concentrations has toxic action on the second-instar larvas of the soybean cyst nematodes, and has obvious inhibition effect on egg incubation. Fermentation stock solution can reduce the number of soybean sporocysts by 59.88%, and has good development and application prospect.

The invention relates to a nematicidal compound which comprises the following active ingredients: a compound in the formula I and iprodione, wherein the weight ratio of the compound in the formula I and the iprodione is 30: 1-1: 20, and preferably, the weight ratio is 10: 1-5: 1. The active ingredients of the nematicidal compound has an obvious synergistic effect in prevention of various crop rootknot nematode diseases within a compound scope and is suitable for preventing various nematodes such as a wheat root knot nematode, a wheat cyst nematode, a citrus root knot nematode, a cucumber rootknot nematode, a tomato root knot nematode, a chili root knot nematode, a peanut root knot nematode, a soybean root knot nematode and the like. The nematicidal compound is good in prevention effect and long in lasting period, so that the nematicidal compound is worthy of promotion and application.

The invention discloses a heterodera avenae derived Ha-18764 protein and a coding gene and application thereof. The protein is obtained from heterodera avenae and named as the Ha-18764 protein and is (a1) or (a2), (a1) refers to a protein formed by an amino sequence shown as a sequence 1 in a sequence table, and (a2) refers to a (a1) derived protein associated with parasitism and / or pathopoiesia and / or development of heterodera avenae and is obtained by subjecting the (a1) to replacement and / or deficiency and / or addition of one or two amino acid residues. The coding gene of the Ha-18764 protein is named as a Ha-18764 gene which plays an important role in a nematode parasitism process and can serve as a target gene for plant nematode resistance engineering. The heterodera avenae derived Ha-18764 protein and the coding gene and application thereof have a significant value in heterodera avenae pathogenesis research and nematode-resistant plant preparation.

The invention discloses a bacillus cereus for preventing and controlling plant nematodes and its application. The name of Bacillus cereus provided by the present invention is MH61‑63‑03, and the preservation number is CGMCC No.15616. The experimental results show that: the fermentation filtrate of Bacillus cereus MH61‑63‑03 has a high poisonous activity against the second instar larvae of plant parasitic nematodes; The number of wheat root nematodes treated with the filtrate decreased, and the population reduction rate was 26.82%. After 65 days of inoculation with the second instar larvae of cereal cyst nematodes, the average number of cysts per plant of wheat treated with MH61‑63‑03 fermentation filtrate also decreased. significantly reduced. The biological agent of the invention has good application and development prospects in the control of plant parasitic nematodes.

The invention belongs to the field of pesticides, and particularly relates to a nematicidal preparation containing abamectin and T.longibrachiatum. The preparation is prepared from an abamectin solution and T.longibrachiatum bacteria liquid, so that an effect of toxically killing wheat cereal cyst nematode can be remarkably improved, a slow release effect is achieved, the lasting period of a pesticide can be prolonged, and the preparation is non-pollution and good in environmental compatibility, achieves low production cost, is capable of preventing nematode from easily becoming resistant to drugs, and has the effects of enlarging a prevention and cure spectrum and improving synergistic interaction, and the like.

The invention discloses a lysine bacillus for preventing and controlling plant nematodes and an application thereof. The name of Lysinibacillus sp. provided by the present invention is BDGS‑17‑07, and the preservation number is CGMCC No.15617. Experiments show that BDGS‑17‑07 fermented liquid has poisonous activity to the second instar larvae of graminearum cyst nematode and the eggs and second instar larvae of root-knot nematode incognita, and also has poisonous activity against egg grains and egg mass hatching of root-knot nematode incognita. The BDGS‑17‑07 fermentation broth also has a good biocontrol effect. Only 50mL of the BDGS‑17‑07 fermentation broth is applied, and the control effect can reach 36% when inoculated with nematodes for 60 days. The Bacillus lysinica BDGS‑17‑07 CGMCC No.15617 of the present invention has good application and development prospects as a biological agent in the prevention and treatment of plant parasitic nematodes.

The invention discloses a Microdochium bolleyi F10 strain microbial inoculum as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) activating a Microdochium bolleyi F10 strain on a PDA (potato dextrose agar) flat plate, then inoculating the strain to a PDA inclined plane for culture, adding sterilized deionized water to prepare a spore suspension, adding tween-20 to ensure that the final concentration of the tween-20 in the spore suspension is 0.025%, and adjusting to ensure that the concentration of the spore suspension reaches 1.0*10<7> / ml; (2) uniformly mixing dry culture material oatmeal with rice husks, adding water, and performing high-pressure steam sterilization; and (3) inoculating the prepared spore suspension to the culture material according to a ratio of 10ml / bag, culturing, airing and crushing to obtain the microbial inoculum. The Microdochium bolleyi F10 strain prepared by using the preparation method has a strong parasitic effect on cysts of heterodera avenae wollenwebers, the control effect of a bio-control microbial inoculum on cereal cyst nematode under a field cultivation condition reaches 41%, an application method of soil treatment is simple and saves labor force, and a biological pesticide is safe to human and livestock.

Disclosed are a Bacillus pumilus strain and the use thereof in preventing and treating cereal cyst nematode in wheat, which strain is a Bacillus pumilus named B202 and deposited in CGMCC with a deposit number of CGMCC NO. 5838. Also disclosed is the use of the Bacillus pumilus strain B202 in the preparation of a biological protective preparation for cereal cyst nematode in wheat. The active ingredients of the biological protective preparation for cereal cyst nematode in wheat comprise at least one of the following (a) or (b) or (c) or (d): (a) a fermentation culture of the Bacillus pumilus strain B202; (b) the Bacillus pumilus strain B202; (c) spores of the Bacillus pumilus strain B202; and (d) a fermentation supernatant of the Bacillus pumilus strain B202. The Bacillus pumilus strain B202 has a stable prevention and treatment effect on cereal cyst nematode in wheat.

The invention relates to a heterodera avenae sensu lato Ha-63744 protein, an encoding gene and the application of the heterodera avenae sensu lato Ha-63744 protein. A sequence of the heterodera avenae sensu lato Ha-63744 protein is shown as SEQ ID NO: (sequence identifier number) 1, and a gene sequence encoding the protein is shown as SEQ ID NO: 2. The Ha-63744 gene is expressed in an esophageal gland of heterodera avenae sensu lato, and an expression level is higher in third and fourth instar larva periods after parasitism of the heterodera avenae sensu lato. After the silent Ha-63744 gene is treated by dsRNA (double-stranded ribonucleic acid), the quantity of the heterodera avenae sensu lato at a wheat root is obviously less than that of a control, which shows that the gene plays an important role in a parasitism pathogenesis process of the heterodera avenae sensu lato and can serve as a target gene of a heterodera avenae sensu lato resistance project of a plant. The protein and the gene have a great value for pathogenic mechanism research of the heterodera avenae sensu lato and preparation of the heterodera avenae sensu lato resistance plant.

The present invention disclosed a kind of vulnerable goat alanine aminotramerase nucleic acid sequence and its application.The present invention is easy to change the goat phenylacease gene to encodes a phenylalanine, which has a high performance of anti -gray cysts, which has important reference and application value for the breeding of wheat anti -gravida cysts.

The invention relates to a rapid detection method of heterodera avenae wollenweber LAMP and application of the detection method. Five LAMP primers, namely HA5-F3, HA5-B3, HA5-FIP, HA5-BIP and HA5-LB are designed and screened according to a heterodera avenae wollenweber random amplified polymorphic dna (RAPD) sequence obtained by cloning; and an LAMP reaction system is configured and optimized. By performing DNA extraction, loop-mediated isothermal amplification, amplified product developing and observation on the heterodera avenae wollenweber, the heterodera avenae wollenweber can be rapidly detected. The detection method is high in specificity, low in cost and convenient to operate, and has high application values on rapid detection of the heterodera avenae wollenweber and early and field diagnosis of the heterodera avenae wollenweber diseases.

The invention discloses a HaHSP4 protein of cereal cyst nematode and a coding gene and application thereof. The protein is obtained from the cereal cyst nematode and named the HaHSP4 protein which isthe protein consisting of the amino acid sequence shown in SEQ ID NO:1 in the Sequence Listing. A gene encoding the HaHSP4 protein is named the HaHSP4 gene, and also belongs to the scope of the invention. The invention also provides the application of the HaHSP4 protein. The application includes regulating the host tropism of the cyst nematode; regulating the parasitic capability of the cyst nematode; regulating the pathogenic capability of the cyst nematode; regulating the development of the cyst nematode. The invention also provides a method for cultivating a plant with improved resistance to the cyst nematode. The method comprises the steps of introducing a substance inhibiting the expression of the HaHSP4 protein into a target plant to obtain the plant with the improved resistance to the cyst nematode. The protein has great value for research on the pathogenic mechanism of the cyst nematode and the preparation of anti-nematode plants.

The invention discloses a caffeine extract of goat grass vulgaris and its extraction method and application, belonging to the field of bioengineering. The specific steps of the extraction method of the caffeine extract of Goatweed vulgaris in the present invention are as follows: take the root of Goatweed vulgaris, wash it, cut it into slices, dry it in an oven at 80°C for 6 hours to remove moisture thoroughly, and then pulverize it with a pulverizer , after passing through a 60-mesh sieve, put 10 grams of pulverized goat grass root samples into a 50-milliliter extraction kettle, and use absolute ethanol as an entrainer. 2 30MPa in the extractor, extract at 60°C for 2h, in which CO 2 The flow rate is controlled at 1.5LPM to obtain the caffeine extract of mutable goat grass. The research of the present invention shows that >0.1% caffeine has a growth inhibitory effect on plant pathogenic nematodes, and the caffeine extract of goat grass vulgaris obtained in the present invention can be used to prepare insect-resistant insecticides for cereal cyst nematodes and root-knot nematodes incognita agent.