Lysinibacillus sp. for controlling plant nematodes and application thereof

A lysine bacillus, plant technology, applied in the application, nematicide, plant growth regulator and other directions, can solve the problems of few chemical nematicides, serious pollution, high toxicity and so on

Active Publication Date: 2018-10-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the control of plant parasitic nematodes is still dominated by chemical control, but there are few varieties of chemical nematicides, and most of them are highly toxic and high residue, which seriously pollute and damage the environment

Method used

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  • Lysinibacillus sp. for controlling plant nematodes and application thereof
  • Lysinibacillus sp. for controlling plant nematodes and application thereof
  • Lysinibacillus sp. for controlling plant nematodes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Isolation, purification and identification of BDGS-17-07 strain

[0044] 1. Isolation and purification of BDGS-17-07 strain

[0045] Strain BDGS-17-07 was isolated from the soil of Badagong Mountain in Zhangjiajie, Hunan Province. The specific steps are as follows: Weigh 5g of soil sample, add it to a conical flask containing 45 mL of sterile water, shake the conical flask in a shaker with a rotational speed of 200rpm / min for 30min, and prepare 10 -1 soil diluent. Dilute step by step in the ultra-clean workbench to prepare a series of corresponding gradient soil dilutions. Pipette an appropriate amount of soil dilution on the LB plate, and then spread it evenly with a spreader, repeating 3 times for each concentration. Placed in a constant temperature incubator at 28 °C for 24 hours, observed the growth of colonies on the plate, picked a single colony on the plate, named it BDGS-17-07 strain, and purified it by three-zone streak on a solid LB plate to obta...

Embodiment 2

[0054] Embodiment 2, the preparation of BDGS-17-07 bacterial agent

[0055] 1. Culture of plate strains

[0056] The BDGS-17-07 strain isolated in Example 1 was inoculated on a LB solid medium plate, cultured at 28° C. for 24 hours, and a single colony was picked to obtain a plate strain.

[0057] 2. Liquid fermentation culture

[0058] The plate strain obtained in step 1 was inoculated into a 250 mL conical flask containing 100 mL of fermentation medium, and fermented and cultured at 200 r / min shaker rotation speed for 60 h at 28 °C to obtain BDGS-17-07 and its metabolites. The fermentation broth was named BDGS-17-07 bacterial agent.

Embodiment 3

[0059] Example 3. Application of BDGS-17-07 inoculum in the control of plant parasitic nematodes

[0060] 1. The nematicidal activity test of BDGS-17-07 inoculum on the second instar larvae of plant parasitic nematodes

[0061] 1. Obtaining the second instar larvae of Meloidogyne incognita

[0062] Take the water spinach roots inoculated with the second instar larvae of Nematode incognita for 60-90 days, wash the root soil with tap water, pick the mature and full egg masses with tweezers under a magnifying glass and place them in the hatching sieve. Place it in a petri dish, add distilled water to the petri dish until the egg mass is slightly covered, and incubate in a 25°C constant temperature incubator. The activity of the second-instar larvae after hatching was collected every 24 hours, and the second-instar larvae after 2-5 days were collected and temporarily stored in a 4°C refrigerator.

[0063] 2. Preparation of second instar larvae suspension of M. incognita and egg ...

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Abstract

The invention discloses lysine bacillus for controlling plant nematodes and application thereof. The name of the lysinibacillus sp. is BDGS-17-07; the collection number is CGMCC No.15617. Experimentalresults prove that the lysinibacillus sp. has the advantages that a fermenting liquid BDGS-17-07 has toxic activity on second instar larvae of cereal cyst nematodes, and eggs and second instar larvaeof meloidogyne incognita, and also has obvious inhibiting function on the hatching of egg particles and egg masses of the meloidogyne incognita; the fermenting liquid BDGS-17-07 also has good biological preventing effect; the preventing effect can reach 36% 60 days after the nematodes are inoculated by only applying 50mL of fermenting liquid BDGS-17-07; when the lysinibacillus sp. BDGS-17-07 (CGMCC No.15617) provided by the invention is used as the biological agent, the biological agent has good application and development prospect in the preventing and control of the plant nematodes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Bacillus lysine for controlling plant nematodes and applications thereof. Background technique [0002] Among the more than 25,000 kinds of nematodes known at present, there are about 4,100 species of plant-parasitic nematodes, accounting for about 15% of the total number of known nematodes. There are many species of plant parasitic nematodes, mainly including Meloidogyne, Anguina, Ditylenchus, Heterodera, Aphelenchoides and root nematodes. Ectoparasitic nematodes, etc. Among them, root-knot nematodes and cyst nematodes are two types of plant parasitic nematodes that cause very serious harm in agricultural production. The disease on vegetables and food crops will cause relatively significant economic losses. [0003] At present, the control of plant parasitic nematodes is still mainly based on chemical control, but there are few varieties of chemical nematicides, and most of them...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P5/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 刘倩简恒王新新
Owner CHINA AGRI UNIV
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