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Microdochium bolleyi F10 strain microbial inoculum as well as preparation method and application thereof

A technology of bacterial strains and bacterial agents, which is applied in the field of plant diseases in Ming Dynasty, can solve the problems of high toxicity, no resistance to nematodes, environmental pollution, etc., and achieve the effects of labor saving, strong parasitic effect, and simple application methods

Inactive Publication Date: 2015-04-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease is a soil-borne disease, and once it occurs, it is difficult to cure it. At present, most wheat varieties are not resistant to nematodes
Chemical pesticides with better control effects are basically highly toxic pesticides, which are highly toxic and cause serious environmental pollution.
Foreign scholars have mostly focused on root-knot nematode biological control research on nematodes, and have successfully developed corresponding biological agents. There are few reports on biological control of wheat cyst nematodes.

Method used

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  • Microdochium bolleyi F10 strain microbial inoculum as well as preparation method and application thereof
  • Microdochium bolleyi F10 strain microbial inoculum as well as preparation method and application thereof
  • Microdochium bolleyi F10 strain microbial inoculum as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Isolation and identification of bacterial strain

[0042] 1. Isolation location and isolation method of the strain:

[0043] The diseased soil where wheat cyst nematode occurred was collected from Niushou Town, Xiangfan City, Hubei Province, and the nematode cysts in the soil were separated by floating. Put a sterilized circular qualitative filter paper + cellophane on the bottom of a petri dish with a diameter of 90 cm, sterilize the surface of the cysts in 70% (volume ratio) ethanol for 2 min, soak in sterile water for 2 min, and place on the filter paper to keep it moist at 25 °C Culture 5d. The fungal hyphae grown from the cysts were purified and cultured on a PDA plate, and the strain was transferred to a PDA slant, and stored in a 4°C refrigerator for later use. The strain was sent to the China Center for Type Culture Collection (CCTCC) located in Wuhan University, Wuhan, Hubei Province for preservation on May 7, 2012, and its preservation number i...

Embodiment 2

[0053] Embodiment 2: Research on the biological characteristics of bacterial strain F10 of the present invention

[0054] 1. The influence of temperature on the growth and sporulation of F10 strain:

[0055] Inoculate F10 on PDA medium, culture at 25°C for 1 day, use a hole puncher (5mm in diameter) to punch out mycelium from the edge of the colony, and transfer it to a Petri dish (90mm in diameter) containing 20ml of PDA In the center, place them at 5°C, 10°C, 15°C, 20°C, 25°C and 30°C for culture respectively, use the cross method, measure the diameter of the colony every 12h, and take out the bacteria with a puncher with a diameter of 6mm in 5 days The silk block was ground, and the yield of conidia per unit area was counted, and 3 replicates were set for each treatment. see results figure 2 , indicating that the F10 strain grows the fastest and produces the largest amount of sporulation under the condition of 20°C-25°C, which is a suitable temperature for growth and spo...

Embodiment 3

[0066] Embodiment 3: the parasitic effect of bacterial strain F10 of the present invention on wheat cyst nematode cyst

[0067] The soil without cysts was taken and sterilized by dry heat at 160°C for 2 hours. Put the sterilized soil into plastic cups, 200g per cup, insert 100 cysts of wheat cyst nematodes, and pour the F10 strain spore liquid every 15 days (concentration is 1.3×10 7 ), watering 10ml each time, using sterile water as the blank control, repeated three times, and cultured in the culture room at 20°C in the dark. After 45 days, the cysts were separated from the soil again, the number of cysts was recorded, and the cyst reduction rate was calculated. . Cyst reduction rate = number of control cysts - number of treated cysts / control cysts × 100%. The number of three repeated cysts decreased by 39, 49, and 55, respectively, and that of the control decreased by 5, 4, and 6. The results showed that in sterilized soil, strain F10 could make the cyst reduction rate of...

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Abstract

The invention discloses a Microdochium bolleyi F10 strain microbial inoculum as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) activating a Microdochium bolleyi F10 strain on a PDA (potato dextrose agar) flat plate, then inoculating the strain to a PDA inclined plane for culture, adding sterilized deionized water to prepare a spore suspension, adding tween-20 to ensure that the final concentration of the tween-20 in the spore suspension is 0.025%, and adjusting to ensure that the concentration of the spore suspension reaches 1.0*10<7> / ml; (2) uniformly mixing dry culture material oatmeal with rice husks, adding water, and performing high-pressure steam sterilization; and (3) inoculating the prepared spore suspension to the culture material according to a ratio of 10ml / bag, culturing, airing and crushing to obtain the microbial inoculum. The Microdochium bolleyi F10 strain prepared by using the preparation method has a strong parasitic effect on cysts of heterodera avenae wollenwebers, the control effect of a bio-control microbial inoculum on cereal cyst nematode under a field cultivation condition reaches 41%, an application method of soil treatment is simple and saves labor force, and a biological pesticide is safe to human and livestock.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, more specifically relates to a bacterial strain Microdochium bolleyi F10 used for the biological control of wheat cyst nematode disease, and also relates to a preparation method of a biocontrol bacterial strain Microdochium bolleyi F10 bacterial agent, and also relates to The application of a Microdochium bolleyi F10 strain in the preparation of medicines for treating or preventing wheat cyst nematode disease. Background technique [0002] Wheat is the most widely distributed food crop in my country and even in the world, and its cultivated area ranks first among food crops. Wheat cyst nematode disease caused by oat cyst nematode (Heterodera avenae) is a dangerous disease that poses a great threat to wheat production and seriously affects the quality and yield of wheat. It was first discovered in my country in 1989, and it is known to have occurred in Hubei, Henan, H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 付艳苹曹冬波姜道宏程家森谢甲涛肖炎农
Owner HUAZHONG AGRI UNIV
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