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Conithyrium minitans strain and application thereof

A technology of shield shell molds and strains, applied in the fields of application, fungicides, fungi, etc., can solve problems such as unfavorable growth of rapeseed and other crops, increased pesticide residues, and human health hazards

Inactive Publication Date: 2017-09-15
WUXI JIANNONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main measure to prevent and control Sclerotinia in my country is to use chemical fungicides. The main problems are: (1) Long-term use of chemical pesticides will cause Sclerotinia to develop drug resistance and increase the amount of pesticides, and the residues of pesticides in crops will continue to rise. High, causing harm to human health and causing a series of problems such as environmental pollution
(2) The use of chemical pesticides to kill Sclerotinia sclerotiorum also killed many natural enemy insects such as bees that impart flowers, which destroyed the ecological balance and was not conducive to the growth of rapeseed and other crops
(3) Due to the particularity of the onset period and site of sclerotinia, as well as the characteristics of intensive planting of crops, traditional pesticide application methods such as spraying cannot effectively reach the main diseased sites such as the roots and stems of crops, and the control effect is greatly reduced

Method used

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  • Conithyrium minitans strain and application thereof
  • Conithyrium minitans strain and application thereof
  • Conithyrium minitans strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the isolation of small shell mold strain

[0021] Weigh 100g of rape field soil samples from 4 different areas into 250mL plastic beakers, bury 50 sclerotia sclerotia and mix well, cover with gauze to prevent pollution, put them into a constant temperature incubator at 20°C, and keep a certain temperature. The sclerotia were taken out after 28 days of soil moisture trap culture. Wash the obviously rotten sclerotia with deionized water, remove the soil on the surface, put it into 0.5% NaClO solution for disinfection for 1 min, and wash it with sterile water for 3 times. Fully mash the washed sclerotia with a sterile glass rod, add sterile water and filter with 4 layers of lens-cleaning paper, dilute the filtrate gradient appropriately and spread it on a PDA plate, and observe the colony growth in the plate after culturing at 20°C for 5 days In some cases, the suspected strains of C. shield were selected for purification until pure culture.

Embodiment 2

[0022] Embodiment 2: Determination of CGMCC NO.8325 to Sclerotinia sclerotiorum putrefactive ability

[0023] Weigh 20g of Jiangsha sieved with 40 meshes and place it in a petri dish, dry heat sterilization at 165°C for 3 hours, put 20 sclerotia sclerotias (approximately 7mm×5mm in size) into each petri dish after cooling , then add the conidia suspension (10 6 cfu / mL), fully stirred and mixed, placed at 20°C and incubated in the dark for 28 days, then taken out to observe the parasitic putrefaction effect of Clamella sclerotiorum on Sclerotinia sclerotia. Each sample was carried out in three groups in parallel, and 4 mL of sterile water was used to replace the spore suspension of the above-mentioned C. shieldii strain as a control group. The rot-causing ability of the Sclerotinia sclerotiorum strain to the sclerotia of Sclerotinia was expressed by the corrected rot-causing rate, and the calculation formula was as follows:

[0024] Rot-causing rate f = number of rotten scler...

Embodiment 3

[0028] Embodiment 3: Determination of CGMCC NO.8325 resistance to chemical fungicides

[0029] Four chemical fungicides including Procymidone, Dimetachlone, Thiram and Iprodione were made into 0.5, 1.0, 1.5, 2.0, 2.5, 5.0 and 10.0 respectively. Drug-containing PDA plates with different concentration gradients such as μga.i. / mL. The 10-day-old CGMCC No.8325 strain was cut with a 12mm hole punch and inoculated to the center of the above-mentioned plate. Five parallel experiments were set up for each concentration of each fungicide, and the PDA plate without fungicide was used as the control group. Place the inoculated plate in an incubator at 20°C for 7 days and measure the colony diameter in each plate with the cross method, subtract the average diameter of the colony obtained from the measurement from the diameter of the inoculated piece to obtain the growth diameter of the mycelium, press Calculate the inhibition rate A (%) of each fungicide concentration on the mycelial gro...

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Abstract

The invention belongs to the technical field of biological pesticides, and concretely relates to a Conithyrium minitans JN-CM strain having a strong parasitic effect on Sclerotinia sclerotiorum. The strain is preserved in China General Microbiological Culture Collection Center in October in 2013, and the preservation number of the strain is CGMCC No.8325. The strain has a good bio-control effect on plant stalk break.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a CGMCC No.8325 strain JN-CM (Coniothyrium minitans) having a biological control effect on plant sclerotinia. Background technique [0002] Sclerotinia is a worldwide distribution of plant disease caused by Sclerotinias clerotiorum (Lib.) de Bary. huge loss. Sclerotinia all occurs during the flowering stage of rapeseed, sunflower and other oil crops, and the diseased site is located in the root of the plant 10 cm above the ground. The development speed is fast, and the diseased area increases exponentially every week. Taking rapeseed as an example, the area to be controlled each year in my country exceeds 50% of the planting area, reaching more than 50 million mu. [0003] At present, the main measure to prevent and control Sclerotinia in my country is to use chemical fungicides. The main problems are: (1) Long-term use of chemical pesticides will cause Sclerotinia to dev...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01N47/38A01N47/26A01N37/32A01P3/00C12R1/645
CPCA01N37/32A01N47/26A01N47/38A01N63/30C12N1/145C12R2001/645A01N2300/00
Inventor 段作营沈志松陈蕴金坚李红李华钟夏森玉
Owner WUXI JIANNONG BIOTECH
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