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Coprinelllus radians and application thereof in preventing and curing cereal cyst nematode

A technology of little ghosts and strains, applied in the biological control of plant diseases and agricultural biology, can solve problems such as harm, and achieve the effects of obvious control effect, stable control effect and simple fermentation process.

Active Publication Date: 2017-02-22
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, chemical control is still one of the important measures to control plant nematodes. Chemical nematicides (such as soil fumigants and non-fumigants) have a certain control effect, but long-term use of highly toxic chemical nematicides has adverse effects on the environment and human health. The dangers are becoming increasingly apparent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: The antagonistic effect of strain HMQAU090439N and its fermentation filtrate on Cereal cyst nematode.

[0035] (1) The parasitism of strain HMQAU090439N on the cysts of Cereal cyst nematode

[0036] Transfer the sterile cysts to the edge of the colony of the bacterial strain HMQAU090439N, 5 per dish, repeat three times, culture for 7 days at 25 degrees, gently pick out the cysts (be careful not to break the cysts), and disinfect the surface with 1% NaClO After washing three times with sterile water for 3 minutes, place on a PDA plate and incubate at 25°C for 5 days. Observe the growth of cysts and record the parasitic rate of the strains on the cysts. The results of the cyst-carrying plate inoculation cyst test showed that the strain HMQAU090439N has a strong parasitic ability on the cysts of Cereal cyst nematodes, with a parasitic rate of 100%, so we can further study its value as a biocontrol strain.

[0037] (2) Toxicity of the fermentation filtrate of strain H...

Embodiment 2

[0040] Example 2: Potted control effect of strain HMQAU090439N on cereal cyst nematode

[0041] Each paper cup was mixed with 5g of wheat kernels and 200g of cereal cyst nematode diseased soil, and one wheat seed was sown in each cup, and cultivated at a constant temperature of 20°C. The paper cup of diseased soil with unmixed wheat kernels was used as a control. The treatment was repeated 5 times, and the infection of wheat roots was investigated on the 60th day. Take out all the wheat roots and soil in the cup, wash them with water, check the roots and the number of white females on the 60-mesh sieve. Calculate the rate of cyst reduction. Using sodium hypochlorite-acid fuchsin staining method, observe the wheat root tissue, count the amount of J2 infection, and determine the fresh weight of the wheat shoots.

[0042] Reduction rate of cysts = ((number of control cysts-number of treated cysts) / number of control cysts)×100%

[0043] J2 infection inhibition rate=((control J2 infect...

Embodiment 3

[0045] Example 3: Field control effect of strain HMQAU090439N on cereal cyst nematode

[0046] When sowing wheat in autumn, seal the bottom end of a plastic tube with a length of 20 cm and a diameter of 4 cm with gauze, add 5 g of bacteria-carrying wheat kernels and 200 g of healthy soil without CCN infection to each tube, sown three wheat seeds per tube and bury them Into the field, with only healthy soil not infected with CCN as a control, each treatment was repeated 5 times. After the wheat emerges, each plastic tube is inoculated with 20 cysts of cereal cyst nematode. In mid-April, we investigated the root infection of wheat. Take out all the wheat roots and soil in the tube, wash them with clean water, check the roots and the number of white females on the 60-mesh sieve. Calculate the rate of cyst reduction. Use sodium hypochlorite-acid fuchsin staining method to observe wheat root tissue and count the amount of J2 infection.

[0047] Field test results showed that on April...

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PUM

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Abstract

The invention belongs to the technical field of plant disease biological control and relates to a Coprinelllus radians strain HMQAU090439N for preventing and curing the cereal cyst nematode. The preservation number of the Coprinelllus radians strain is CGMCC No.11814. The invention also discloses a microbial inoculum prepared by the above strain, and the application thereof in preventing and curing the cereal cyst nematode. According to the invention, an efficient microorganism for preventing and curing the cereal cyst nematode is provided. The Coprinelllus radians strain HMQAU090439N is good in parasitic and antagonism effect for the nematodes of the cereal cyst nematode. Meanwhile, the results of the pot experiment and the plot experiment show that, the Coprinelllus radians strain HMQAU090439N is good in control effect for the nematodes of the cereal cyst nematode. In addition, the microbial inoculum is safe to human bodies and livestock and is environment-friendly, thus having a good development and application prospect.

Description

Technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to the screening, fermentation, nematicidal activity determination of a strain of Coprinelllus radians HMQAU090439N which has parasitic and toxic effects on plant nematodes, and the determination of its nematicidal activity on wheat and cereal cysts The application of nematode disease prevention and control belongs to the field of agricultural biotechnology. Background technique [0002] Plant pathogenic nematodes are a serious plant disease that causes nearly US$157 billion in economic losses to the world’s agriculture every year. More than 5,000 species of plant pathogenic nematodes have been reported in more than 200 genera. Among them, Heteroderaavenae (Heteroderaavenae) is an important pathogenic nematode that harms wheat and other wheat crops, and 40 in Europe, Asia, Africa, North America, South America and Oceania. It is distributed in many countries,...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/68A01N63/04A01P5/00C12R1/645
CPCA01N63/30C12N1/14C12Q1/6895C12Q2600/158C12N1/145C12R2001/645
Inventor 梁晨赵洪海李宝笃杨小凤黄晨
Owner QINGDAO AGRI UNIV
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