33 results about "Cellular mechanism" patented technology

The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present invention provides numerous improvements over previous mutagenesis methods, such advantages include that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA in-to host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.

Compositions and methods for targeted gene therapy are disclosed. Compositions containing double duplex-forming pseudocomplementary oligonucleotides are administered in combination with a donor oligonucleotide that is homologous to a target sequence on a double-stranded DNA molecule in need of repair or replacement. By activating cellular mechanisms involved in DNA synthesis, repair and recombination, the double duplex-forming pseudocomplementary oligonucleotides can introduce one or more mutations at a site of interest by increasing the efficiency of targeted recombination of the donor oligonucleotide. The pseudocomplementary oligonucleotides / donor oligonucleotide compositions may be administered in combination with a second therapeutic agent that enhances access of the pseudocomplementary oligonucleotides and / or the donor oligonucleotide to the target site, an agent that enhances or increases DNA repair or recombination, or an agent that enhances uptake or delivery of the oligonucleotides.

The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present invention provides numerous improvements over previous mutagenesis methods, such advantages include that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA into host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.

A treatment solution used to prevent posterior capsular opacification is applied or introduced into the lens capsular bag before, during, or after cataract surgery. The treatment solution comprises an ion transport mechanism interference agent, which either alone or in combination with other treatment agents such as an osmotic stress agent and an agent to establish a suitable pH, selectively induces detachment and / or death of lens epithelial cells such that posterior capsular opacification is prevented. While the ion transport mechanism interference agent is capable of interfering with the cellular mechanisms and cell ion distribution of a broad range of cells, a concentration of agent is selected such that the treatment solution interferes selectively with the cellular mechanisms of lens epithelial cells while leaving other ocular cells substantially unharmed. The treatment solution selectively induces cellular death and / or detachment of lens epithelial cells while other ocular cells and tissue remain substantially unharmed and without lengthy preoperative pre-treatment.

A treatment solution used to prevent posterior capsular opacification is applied or introduced into the lens capsular bag before, during, or after cataract surgery. The treatment solution may also be applied to an intraocular lens prior to surgery. The treatment solution comprises an ion transport mechanism interference agent, which either alone or in combination with other treatment agents such as an osmotic stress agent and an agent to establish a suitable pH, selectively induces detachment and / or death of lens epithelial cells such that posterior capsular opacification is prevented. While the ion transport mechanism interference agent is capable of interfering with the cellular mechanisms and cell ion distribution of a broad range of cells, a concentration of agent is selected such that the treatment solution interferes selectively with the cellular mechanisms of lens epithelial cells while leaving other ocular cells substantially unharmed. The treatment solution selectively induces cellular death and / or detachment of lens epithelial cells while other ocular cells and tissue remain substantially unharmed and without lengthy preoperative pre-treatment.

Disclosed herein is a three-dimensional culture containing human articular chondrocytes with induced terminal differentiation changes, as well as a process for preparing the same. The three-dimensional culture, which was found to mimic the biological characteristics of articular chondrocytes that undergo terminal differentiation in vivo, can be used as a tool in the studies of the molecular and cellular mechanisms of osteoarthritis, and in the screening of candidate drugs for use in treatment of a disorder associated with articular chondrocytes.

The invention discloses a scalable robot trunk and a bionic quadruped robot, comprising: a trunk link and an annular metamorphic mechanism; the trunk link connects two structurally symmetrical annular metamorphic mechanisms; the annular metamorphic mechanism consists of A plurality of first connecting rods are movably connected head-to-tail by the first connecting piece, and the second connecting rod is connected at the junction of every two first connecting rods, and only one end of each first connecting rod is connected with the second connecting rod, so The second connecting rods are movably connected through the second connecting piece; the telescopic rod is connected between the two second connecting rods in the center symmetrical position of the annular metamorphic mechanism. The present invention adopts a structurally symmetrical foldable metamorphic mechanism, two metamorphic mechanisms are connected through a trunk link to form a robot trunk, and each metamorphic mechanism adjusts the radial dimension of the robot trunk through a telescopic rod; the leg mechanism and the trunk Telescopic motion coupling, through the scaling and deformation of the torso, the distance between the legs of the robot, the height of the torso from the ground, etc. can be adjusted.

The present invention relates to isolated human cells that are precursors of endothelial cells other than embryonic stem cells, to isolated murine cells that are precursors of endothelial cells, and to methods using same. In particular, the present invention relates to established cell lines of isolated human cells that are precursors of endothelial cells other than embryonic stem cells and established cell lines of isolated murine cells that are precursors of endothelial cells. The present invention can be used in particular in the medical and / or veterinary fields, in particular in the field of therapeutics and / or in the field of studies of cellular mechanisms.

The invention relates to the technical field of extensible cells, and particularly discloses a single-degree-of-freedom tetrahedral extensible cell mechanism. The mechanism comprises a first connecting rod, a first rotating pair, a second connecting rod, a second rotating pair, a third connecting rod, a fourth rotating pair, a fourth connecting rod, a third rotating pair, a ninth rotating pair, aneighth connecting rod, a tenth rotating pair, a ninth connecting rod, an eleventh rotating pair, a seventh rotating pair, a seventh connecting rod, a sixth connecting rod, an eighth rotating pair, asixth rotating pair, a fifth connecting rod and a fifth rotating pair. The mechanism comprises nine connecting rods and eleven rotating pairs, the single-degree-of-freedom tetrahedral extensible cellmechanism has the advantages that the structure is simple, motion pairs are fewer, rotating pairs are completely adopted, and the mechanism precision is higher.

Disclosed is a metamorphic mechanism type obstacle crossing crab-like robot. Six magic cube center rotating blocks of the crab-like robot are mounted on a magic cube center cross-shaped block and can rotate around the magic cube center cross-shaped block. First magic cube edge blocks and second magic cube edge blocks are clamped into the vacant positions between the magic cube center rotating blocks, and first magic cube angle blocks and second magic cube angle blocks are clamped into the remaining vacant positions. First bearings are mounted in holes of the second magic cube edge blocks, and second bearings are mounted in holes of the second magic cube angle blocks. One ends of metamorphic rods are mounted on the magic cube center rotating blocks, and the other ends of the metamorphic rods are mounted on metamorphic operating rods. The crab-like robot is a flexible and changeable mechanism, objects can be stably borne, products can be operated in space by changing the degree of freedom through metamorphism, and the structure state can further be flexibly changed to cross obstacles.