34 results about "Bud dormancy" patented technology

The invention relates to a plant sprouting agent and a preparation method thereof. The materials of the sprouting agent are 6-benzyladenine, kinetin, gibberellin, dimethylsulfoxide, glycerol and lanolin; the dimethylsulfoxide solution and the glycerol are added into a glass beaker and dissolved, so that hormone solution is obtained; the lanolin is put into another big beaker, and is heated to 70 DEG C to be melted, and as the lanolin is stirred to be slowly melted, the hormone solution is slowly added into the lanolin and sufficiently and uniformly stirred; and after being cooled to the room temperature, the hormone mixture is coagulated into paste, which is the high-effective plant sprouting agent. Each component in the sprouting agent can rapidly penetrate the cell membranes of the dormant buds of plants and directionally enter cells, consequently, the efficacy is enhanced, and the acting time is shortened. The plant sprouting agent can increase the plant sprouting speed, shorten the growing time and accelerate seedling formation; bud dormancy is broken, so that the growth of plants is increased; the plant sprouting agent can change the hormonal balance in plant cells, promote sprouting and accelerate the elongation growth of the stems and leaves of plants; and the plant sprouting agent has a moisture-keeping effect, which can help to protect the new sprouts of plants.

The invention relates to the field of protection of plant germplasm resources, and in particular provides a method for ultra-low temperature preservation and grafting resurrection of dormant buds of mulberry trees, including 1) obtaining dormant branches; 2) cutting into dormant buds; 3) drying; 4) sealing; 5) Gradient cooling; 6) Liquid nitrogen preservation; (7) Thawing; 8) Rehumidification; 9) Grafting resurrection. The method of the invention is a method for ultra-low temperature preservation and grafting resurrection of dormant buds of mulberry trees by using a drying-gradient cooling and freezing method. The dormant buds of mulberry trees are dried to a water content of 20%-40%, and a higher survival rate is obtained through steps such as slow and step-by-step cooling treatment, freezing storage, thawing, rehumidification, and grafting and resurrection. By adopting the method provided by the invention, the average survival rate of dormant buds of mulberry trees after cryopreservation can reach up to 95%. The cryopreservation method for mulberry dormant bud segments provided by the invention has simple procedure, easy operation and good freezing effect, and is an effective way for long-term preservation of mulberry germplasm resources in vitro.

The invention discloses a medicament for breaking the dormancy of winter buds of grapes and making the fruit ripen in advance. The medicament comprises: sodium tungstate 0.5%-2%w / v, sodium nitroprusside 0.03%-0.09%w / v, potassium sulfate 1 %‑3%w / v, diammonium phosphate 1%‑3%w / v, 5‑azacytidine 0.2%‑0.8%w / v, brassinolide 0.01%‑0.04%w / v. The invention solves the lack of a non-toxic or low-toxic agent in the prior art, which can effectively break the dormancy of winter buds of grapes, promote early germination and early ripening of fruits. The invention also discloses a method for breaking dormancy of winter buds of grapes and ripening fruits in advance. The above-mentioned medicine is evenly applied to winter buds of grapes or evenly sprayed on branches and winter buds of grapes. The phenological phases such as the ripening period and fruit ripening period were all advanced in varying degrees, and there was no adverse effect on the germination rate, branching rate and fruiting branch rate of grape winter buds after treatment.

The invention relates to a method for breaking peony bud dormancy and belongs to the technical field of plant cultivation. The method comprises the following steps: 1) after putting peony in a pot androots are raised, pouring GA3 into a peony pot; 2) putting the potted peony at the leeward and sunny position after pouring the GA3, and keeping warm for the potted peony when the lowest air temperature is reduced to 2 to 4 DEG C; 3) when the daily minimum air temperature is less than -2 DEG C, immigrating the potted peony into an unheating greenhouse, starting to heat in the middle of December,increasing the room temperature to 10 to 20 DEG C after stem leaves grow, controlling the temperature to 15 to 25 DEG C in the bud growing state and later, heating the greenhouse every day and supplementing light simultaneously. A series of special operation processes of entering a storage to refrigerate, going out of the storage and entering the greenhouse are not needed by utilizing gibberellinsand a natural low-temperature method, the process of breaking the peony bud dormancy is greatly simplified, and the cost is reduced; meanwhile, compared with the traditional refrigeration storage refrigeration method, the method has the advantage of leading the flowering phase of the peony 15 to 20 days in advance.

The present invention discloses a method for regulating and controlling flowering phase of peony. Said method includes the following steps: selecting and using biennial peony plant with plump floral buds, cold-storing said peony plant for 2-8 weeks at 0-4 deg.C to break its top-end floral bud dormancy, making flowering management, when the buds are getting ready to burst or after its full blossomis completed, taking measures to make non-sprouted buds begin or start an action, more making flowering management. The invented method can make pot-cultured peony have two flowering phases in 1-4 months continuously.

The invention relates to the field of molecular biology and in particular discloses application of Pm011905 genes (SEQ ID NO.1) in an aspect of monitoring a prunusmume flower dormancy state and a molecular detection method for a prunusmume flower bud dormancy state. The method comprises the following steps: (1) collecting flower buds of to-be-detected prunusmume flower twice, respectively extracting RNA of the flower bud and performing inverse transcription to obtain cDNA, and by taking the cDNA as a template, carrying out a fluorescent quantitative PCR (Polymerase Chain Reaction) on Pm011905 genes by utilizing primers shown as SEQ ID NO.2-3; and (2) comparing the expression quantity of the Pm011905 genes of the flower buds collected twice, and judging the dormancy stage of the prunusmume flower buds. The method disclosed by the invention first discovers that the dormancy state of the flower buds can be monitored or judged by virtue of the change of the expression quantity of the Pm011905 genes of the flower buds. The invention provides a simple, convenient, rapid and accurate method capable of detecting the prunusmume flower bud dormancy state on a molecular level.