43 results about "Auto immunite" patented technology

The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.

The invention relates to the technical field of immunohistochemistry, in particular to a fully automatic immunohistochemical system. The fully automatic immunohistochemical system includes a workbench, a sample loading table, a refrigerating component, a repairing component, an incubating component, a slide moving mechanism and a sample loading mechanism. The refrigerating component is arranged onthe workbench; and the refrigerating component includes a plurality of reagent bottles for storing reagents and a refrigerating unit for refrigerating the reagent bottles. A slide moving arm of the slide moving mechanism is arranged to grasp, rotate and transfer a slide module, and a sample loading arm is configured to load samples to different slide modules; and the cooperation of the slide moving arm and the sample loading arm can reduce human operations to a greater extent, so that the process of immunohistochemistry is more automated; and therefore, the machine throughput can be greatly increased. In addition, the refrigerating component is arranged on the workbench to achieve low-temperature storage of the reagents, and the reagents do not need to be taken repeatedly; therefore, theexperimental efficiency can be greatly improved. Through the fully automatic immunohistochemical system, the experimental throughput can be greatly increased, and the automated immunohistochemical experiment process can be more optimized.

The present invention utilizes an extracting reagent to achieve quantitative detection of hydrophobic analytes from a biological test sample. The hydrophobic analytes can include steroids, and drugs. The biological test sample can include serum, plasma, whole blood, urine and spinal fluid. The hydrophobic analyte is preferably the drug cyclosporine, which is used primarily as an immunosuppressant. The biological test sample is preferably whole blood. The extracting reagent comprises a zwitterionic detergent and saponin, and optionally, a viscosity additive such as sucrose or glycerin. The detection and analysis of the hydrophobic analyte is preferably performed in an automated immunoanalyzer.

The invention relates to an automatic immunity analyzer which comprises a rack, a main control module, at least one cup body transfer module, at least one sample adding module, at least one reagent adding module and at least one sampling detection module, and a plurality of station bins are arranged on the rack; the cup body transfer module is used for driving the reaction cups to transfer among the station bins; the sample adding module comprises a sample needle for adding a sample; the reagent adding module comprises a reagent needle for adding a reagent; the sampling detection module comprises a detection needle used for extracting a sample and a detection assembly connected with the detection needle. By adopting an assembly line type structure, integrated operation of sample adding, incubation, reagent adding, uniform mixing, cleaning and detection can be realized, the automation degree is relatively high, the operation difficulty is reduced, and the detection efficiency is improved.

The invention discloses a semi-automatic immunofluorescence analyzer. The analyzer comprises a sample module, a reagent module, an incubation module, a sampling and sample adding module and a detection module, wherein the reagent module and the incubation module are integrated on the same platform structure and can move along a track, and the platform structure is provided with a plurality of side-by-side slot positions and a plurality of rows of row holes; a reagent card is arranged in each slot position so as to form the incubation module with a plurality of incubation positions, and the rowholes are provided with a plurality of hole positions so that at least one TIP head and one reagent tube can be inserted into the reagent module; the sampling and sample adding module can move alongthe track; and the detection module moves along the track so as to carry out immunofluorescence detection on the different reagent cards. In the invention, the reagent module and the incubation moduleare integrated on the same platform structure; and the sampling and sample adding module can complete sampling and sample adding actions with small displacement along the track, sampling and sample adding time is shortened, meanwhile, the sampling and sample adding module moves along the track, automatic control is facilitated, sampling and sample adding efficiency is improved, and the influenceof manual operation of sampling and sample adding on stability of a detection result is eliminated.

The invention relates to a full-automatic immunoblotting instrument and a detection method. The full-automatic immunoblotting instrument comprises a rack, wherein kits, a reagent filling device, reaction vessels with membrane strips, sample boxes, and a sample filling mechanism used for adding sample liquid in the sample box into the reaction vessel are arranged on the rack. A large disc and a small disc which are coaxial are rotationally arranged on the rack; the plurality of kits are arranged on the small disc along the circumferential direction of the small disc; the reagent filling deviceis positioned at the outer edge of the small disc; a plurality of reaction vessels and a plurality of sample boxes are arranged in the circumferential direction of the large disc; the sample boxes arearranged on the edge of the large disc; the reaction vessels are installed between the center of the large disc and the sample boxes, and the sample filling mechanism is arranged on a short-stroke rail and moves in a radial direction of the large disc. The space structure of the large disc and the small disc is reasonably and efficiently utilized, the whole instrument tends to be miniaturized, efficient and automatic, a disposable suction nozzle is clamped, and cross infection is avoided.

The invention relates to the field of immunological detection, in particular to a diluted composition and a reagent or a kit for osteopontin detection. The kit for detecting osteopontin by a magneticparticle chemiluminescence method comprises a magnetic particle suspension coated with an osteopontin monoclonal antibody, an enzyme conjugate containing the osteopontin monoclonal antibody, a samplediluent and a calibrator. The kit is prepared based on the magnetic particle chemiluminiscence method, is used in cooperation with an Auto Lumo series full-automatic immunoassay instrument, can achieve automatic detection, and is easy and convenient to operate and high in detection speed. The kit has the advantages of wide detection range, high sensitivity and strong specificity, can quantitatively detect the content of osteopontin in serum and plasma, and is used for early diagnosis, disease process monitoring, treatment effect evaluation and prognosis monitoring of gastric cancer, lung cancer, liver cancer, breast cancer and the like.

Cell based assays are required for the detection of biological activity and hence are required for use as potency assays, detection of neutralizing antibodies and detection and quantification of the effector cell function of therapeutic antibodies or the quantification of the potency or neutralizing antibody response to virus vectors such as AAV vectors used in gene therapy. Cell-based assay are also required for the quantification of antibody mediated effector functions including complement-dependent cytotoxicity (CDC), antibody-dependent cellular phagocytosis (ADCP), and antibody-dependent cell-mediated cytotoxicity (ADCC). Cell based assays are difficult to adapt for use on automated immuno-assay platforms. Reporter-genes that encode readily visible proteins that can be quantified using fluorescence or luminescence such as luciferases (firefly, Renilla, Gaussia, Nano Luciferase etc), fluorescent proteins such as green fluorescent protein (GFP) or dsRED or an enzyme such as chloramphenicol acetyltransferase (CAT) or a protease and respond to signal transduction, that is directly related to the mechanism of action of a drug, can be used to quantify the potency of a drug following binding of a drug to a soluble target or cell surface target (receptor or other cell-surface molecule), anti-drug neutralizing antibodies, and therapeutic antibody induced effector cell function. The principal of the invention is that reporter-gene product or by-product produced either during the course of the cell-based assay or onconclusion of the cell based assay is quantified either in the cell medium or cell supernatant, for a secreted protein, or following lysis of the cells with a suitable passive lysis buffer. The reporter-gene product such as firefly luciferase is then detected in the cell medium or cell supernatant or cell lysate using an antibody pair (monoclonal or polyclonal) specific for the gene product such as a firefly luciferase labelled with the dual detection system specific for an ELISA or a particular automated assay platform such as Meso Scale Discovery electro-chemiluminescence (MSD-ECL), Luminex, SMC, Alpco, AlphaLISA, Gyros or label free detection using SPR such as the Biacore platform. The expression of the reporter-gene product such as firefly luciferase can be normalized with respect to the expression of a second reporter gene product such as Renilla luciferase or Nano Luciferase under the control of a constitutive promoter.

The invention discloses a no-microspheres homogeneous chemiluminiscent system of biotin-avidin or streptavidin as well as a method for applying the system to quantitative antigen or antibody measurement, and relates to the technical field of chemiluminiscent systems. The no-microspheres homogeneous chemiluminiscence system comprises a biotin-labeled antibody or antigen, a 9, 10-dihydracridine-labeled antibody or antigen, and HRP-labeled avidin or streptavidin. According to the system, a homogeneous system without microspheres is used; the biotin-avidin or streptavidin system is used to greatlyimprove the chemiluminiscence efficiency and detection sensitivity, the lowest detection concentration of the example S100B protein can reach 0.015 ng / ml; and a detection process of the system does not need cleaning, is convenient and simple, and can be used to an automatic immune homogeneous chemiluminescence system, POCT, a micro-fluidic chip and the like.

The invention discloses a full-automatic immunohistochemical instrument cover glass washing method. The full-automatic immunohistochemical instrument cover glass washing method comprises the followingsteps that after the surface of cover glass obtained after immunohistochemical detection through a full-automatic immunohistochemical instrument is thoroughly flushed by tap water, a potassium permanganate solution with the concentration being 0.5-5%, a sodium metabisulfite solution with the concentration being 0.5-2% and a Tween_20 solution with the concentration being 1-10% are sequentially used for flushing, and the thoroughly-cleaned full-automatic immunohistochemical instrument cover glass is obtained. The disclosed full-automatic immunohistochemical instrument cover glass washing methodhas the advantages that fading is thorough, the dirt removing capability is high, the service life of the cover glass is prolonged, and cost is saved.

The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.

An automated immunoassay apparatus is disclosed comprising a single optical reading device for reading two microtitre plates. A first microtitre plate is loaded into an upper plate holder which is linearly translated at a fixed height. A second microtitre plate is loaded into a lower plate holder. The lower plate, holder runs along a contoured track which varies the vertical height of the second microtitre plate.

The invention belongs to the field of medical instruments, and discloses a full-automatic immunity analyzer and an analysis method thereof. Samples are stored on a sample storage and transfer assembly, reagents are stored on a reagent loading assembly, and a certain amount of clean empty reaction cups are borne on a reaction cup supply assembly; a dispensing assembly dispenses quantitative samples and reagents into the reaction cups from the sample storage and transfer assembly, the reaction cups containing the samples and the reagents are uniformly mixed through a uniform mixing assembly, and the sample reagents are fully dispersed; a reaction disc assembly performs constant-temperature incubation on the uniformly mixed reaction cups; a magnetic separation and cleaning assembly is used for cleaning and separating the reaction cups after constant-temperature incubation of the reaction disc assembly is finished, an optical measurement assembly is used for measuring optical signals of the reaction cups which are added with substrates and have stable reaction, and a waste liquid recovery assembly is used for absorbing waste liquid of the reaction cups with measured optical signals, so that the whole cycle time is shortened, the test flux is improved, the operation process is simple, and resources are saved.

The invention discloses a full-automatic immunoblotting instrument and a using method thereof.The full-automatic immunoblotting instrument comprises a rack, a sample module, a uniform mixing module, a liquid adding and absorbing module, an air drying module, a sample adding module and an image collecting module, the rack comprises a rack body, a door, a first side plate, a second side plate and a top plate, and the door is installed on the rack body; a sample module, a uniform mixing module, a liquid adding and absorbing module, an air drying module, a sample adding module and an image acquisition module are mounted in the rack main body. According to the invention, full-automatic sample adding and liquid adding, film strip air drying and result interpretation can be realized, manpower is saved, the device is more convenient, and automatic treatment is realized; the noise during treatment is reduced, the cost rise caused by using a high-power fan is avoided, and the phenomenon that the film strip is blown back or away from the correct position is also avoided; the film groove can always face the fan, so that the air drying efficiency is improved; more stable opening and closing of the door are realized; and the recovery of the TIP head is realized.

The invention belongs to the technical field of motor drive, and discloses a high-speed centrifugal turntable of an automatic immune analyzer and a positioning control method, comprising a centrifugal turntable, a motor driver, a brushless DC motor, a reagent card slot and an absolute encoder; the quality of the centrifugal turntable is concentrated in At the central axis, the outer edge is a fan-shaped strip structure to minimize the mass and reduce the moment of inertia under the condition of a certain radius; the DC brushless motor accelerates the open-loop operation according to the S-shaped curve to a specific speed, which drives the direct-connected installation. The centrifugal turntable on the motor output shaft rotates to separate the samples in the reagent card slot. After the separation is completed, the motor speed is reduced. When the speed is reduced to the set speed, the motor moves in a closed loop. The device detects the position of the centrifugal turntable, and uses the PID control algorithm to precisely control the centrifugal turntable to stop at the specified position, which improves the positioning accuracy of the high-speed centrifugal turntable of the automatic immunoassay analyzer and facilitates the subsequent processing of the sample by the robotic arm.

An instrument for detecting signal from a biological sample includes a pipettor module configured to hold a plurality of pipettes in respective pipette positions, to hold liquid in one or more pipette tips, and to pipette liquid in and out of the one or more pipette tips. Each of the one or more pipette tips has a pipette tip point. The instrument further includes one or more magnets positioned such that each of the one or more pipette tips is adjacent one of the one or more magnets.

A cleaning buffer solution adapted to a full-automatic immunohistochemical staining machine and a preparation method thereof are characterized in that the cleaning buffer solution is formed by dissolving a prepared cleaning buffer solution A in purified water, the cleaning buffer solution A comprises the following main components: tris (hydroxymethyl) aminomethane, sodium chloride, Tween-20 and ProClin950, and the pH value range of the cleaning buffer solution A is 7.3-7.5. The technical scheme of the invention has the advantages that the use of sodium azide can be avoided, the consistency, stability and accuracy of the detection result are ensured, and the dyeing effect and the cleaning effect can be improved.

The invention belongs to the technical field of automatic control of medical equipment, and discloses a motor control system and a medical detection system of a fully automatic immune analyzer, including: a host computer for collecting control information; a master control card for receiving and analyzing the host computer The control information sent controls the precise movement of the motors of each module; the power distribution management and bus monitoring module is used to integrate the data line with the communication line, monitor and upload the running status in real time; the sample adding module is used to realize medical clinical testing. Reagent sample addition operation; centrifugation module, used to realize high-speed centrifugation of reagents in medical clinical testing; temperature control module, used to realize constant temperature incubation of reagents in medical clinical testing; dual robotic arm cooperative control module, used to realize medical clinical testing Reagent removal card puncture operation. The invention makes the wiring of the system simple and compact, eliminates accumulated errors, improves the precision of motor control, and improves the efficiency and positioning precision of the automatic immune analyzer.

The full-automatic immunoassay system comprises a machine body, a through opening is formed in one side of the outer wall of the top of the machine body, a uniform mixing mechanism is arranged in the through opening and comprises a rotary disc and a storage rack, a cavity is formed in the machine body, a through hole is formed between the cavity and the through opening, and the rotary disc is arranged in the through hole. A buffering mechanism is arranged on one side of the inner wall of the bottom of the cavity, a supporting plate is arranged on the outer wall of the top of the buffering mechanism, a rotating rod is rotationally connected to the outer wall of the top of the supporting plate through a bearing, the rotating rod is fixedly connected with the rotating disc, and the rotating rod is slidably connected with the through hole. The situation that when samples are evenly mixed, centrifugal force generated by rotation causes shaking of the reagent tubes, the samples are scattered from the interiors of the reagent tubes, even mixing of the samples is affected, and then deviation of immunoassay data results is caused is prevented, the fixing effect of the reagent tubes can be conveniently improved, the even mixing effect of the samples can be improved, and the accuracy of immunoassay data is improved. Therefore, the accuracy of immunoassay is improved.

The invention relates to an automatic immunoblotting analyzer, comprising: a reagent module, a sample injection module, a material addition module, a cleaning module, an incubation module, a liquid injection and suction module, an interpretation module, and a drying module. At the same time, a fully automatic immunoblotting analysis test method is also provided. The beneficial effects of the present invention are as follows: with the incubation module as the core, the other modules are arranged around the incubation module, according to the tested items, the sample injection module, the reagent module and the feeding module are used to realize automatic sample feeding and automatic addition of reagents, and the cleaning module is used to The feeding module is cleaned to avoid contamination between the agents. The incubation module is used to inject the cleaning solution and the waste liquid is removed. The drying module is used to dry the incubation module. Image analysis and result output do not require manual participation in the test process, and each module cooperates with each other to realize the whole process from sample injection to result output.

The invention discloses a kit and a method for detecting an antibody by a spatial proximity chemiluminescence two-step method. The kit comprises a luminous marker, an enzyme marker, a biotin-labeled antigen solid-phase coating substance, an auxiliary agent, a triggering agent and a calibrator; the luminous marker comprises a 9,10-dihydroacridine labeled anti-human immune globulin monoclonal antibody; and the enzyme marker comprises the raw material component of HRP-labeled avidin / streptavidin. According to the invention, a streptavidin / biotin system is introduced into a detection system, and a plurality of clinical projects requiring a two-step method to complete detection can be completed by applying a spatial proximity chemiluminescence technology, so that the clinical application range and value of the spatial proximity chemiluminescence technology are improved; meanwhile, a biotin-avidin / streptavidin system is introduced, so that the chemiluminescence efficiency can be greatly improved, and the detection sensitivity is improved; and the system can be used for rapid quantitative analysis of an automatic immunochemiluminescence system, POCT, a micro-fluidic chip and the like.

The invention relates to a full-automatic immunohistochemical staining instrument and a staining method thereof.The full-automatic immunohistochemical staining instrument comprises a rack, a staining box assembly, a reagent bottle assembly, a movable sample adding needle, a universal reagent sample adding assembly and a waste liquid collecting box; the dyeing box assembly is installed on the rack and comprises a dyeing box and a driving mechanism used for driving the dyeing box to shake, the dyeing box is installed on the driving mechanism and comprises a container and a cover body, the two side walls of the container are provided with an opening used for installing a slide and a liquid discharging opening used for discharging liquid respectively, and the waste liquid collecting box is arranged on the liquid discharging opening side of the dyeing box; the reagent bottle assembly is installed on the rack and comprises a reagent bottle rack and reagent bottles, containing grooves used for installing the reagent bottles are evenly formed in the top of the reagent bottle rack, and the reagent bottles are used for containing different reagents; the contact efficiency of tissues and reagents is enhanced through a movement device, the reaction time of primary antibodies and secondary antibodies is shortened, and the device is suitable for further popularization and application.

The invention discloses a microsphere-free homogeneous chemiluminescence system of biotin-avidin or streptavidin, and a method for quantitatively measuring antigens or antibodies, and relates to the technical field of chemiluminescence systems. The microsphere homogeneous chemiluminescence system includes: biotin-labeled antibody or antigen, 9,10-dihydroacridine-labeled antibody or antigen, HRP-labeled avidin or streptavidin. Biotin-avidin or biotin-streptavidin No Microspheres Honogeneous Luminescent (Biotin-Avidin / streptavidin) system does not use microspheres, it is a homogeneous system; the introduction of biotin ‑Avidin / streptavidin system can greatly improve chemiluminescence efficiency and detection sensitivity. The minimum detection concentration of S100B protein in the example can reach 0.015ng / ml; the detection process of this system does not need cleaning, is convenient and simple, and can be used in automatic Immunohomogeneous chemiluminescence system, POCT and microfluidic chips, etc. for rapid quantitative analysis.

The invention relates to the field of chemical analysis, and discloses a full-automatic immune and biochemical integrated analyzer and a use method thereof. The device comprises a common part, an immunization part and a biochemical part, the common part comprises a sample reagent loading assembly and a dispensing assembly; the immunization part comprises an immunization reaction disc assembly, an immunization uniform mixing assembly, an immunization photometry assembly, a reaction cup supply assembly and a reaction cup transfer assembly; the biochemical part comprises a biochemical reaction disc assembly, a biochemical stirring assembly, a biochemical stirring and cleaning assembly, a biochemical cleaning hand assembly and a biochemical light path detection assembly. According to the invention, the common part is used for sample addition, the immune part is used for chemiluminescence immune detection, and the biochemical part is used for biochemical detection, so that the working efficiency can be improved, the occupied space can be reduced, and the purchase cost can be saved.

The present invention relates to an automated immunoassay device and method. The automated immunoassay device comprises: a cartridge (13) in which a T tip tube (9), a reactive tube (11), a washing tube (3), and a signal measuring tube (5) are integrally connected to one another or separately arranged; a T tip (7) and a magnetic rod (31) which enter the multiple tubes (3, 5) sequentially to allow the migration of large magnetic particles (m), a capturing material, a signal material, and an analysis material by magnetic force, with the concomitant performance of reactions and washing processes;a cartridge holder (15) on which the cartridge (13) is stably placed; and a heating member (17) disposed in an area in which the reactive tube (11) is stably placed on the cartridge holder (15) so asto generate heat, wherein the large magnetic particles (m) comprise morphologically identical or different magnetic particles to which a material capable of capturing only a specific analysis materialper each of the morphologically different magnetic particles is coupled.

The invention provides a semi-automatic immunofluorescence analysis system, which includes a test card delivery device, an optical path structure, and an optical analysis device including the optical path structure. The invention has high sensitivity and accuracy. By shortening the distance between the convex lens and the detection area, the fluorescence with a larger angle range can enter the detection optical path, thereby improving the detection sensitivity and accuracy of the system. The structure is exquisite. The invention has the advantages of compact structure, good light density, high reliability, convenient assembly, and high-efficiency detection at low cost. It realizes semi-automatic accurate monitoring and fills the gap in this field.

The invention discloses a method for batching and scheduling mixed optimization of fully automatic immune testing equipment, comprising the following steps: S1, reading the information of the blood sample to be tested, reading the information of the sub-testing machine in the equipment, and reading the information of the item to be tested from the database Inspection step information; S2. Establish a hybrid optimization model of sub-batch division and inspection sequence scheduling based on the data obtained in step S1; S3. Perform hybrid optimization based on the multi-population cooperative migratory bird migration algorithm for sub-batch division and inspection sequence scheduling to obtain the total inspection time The shortest sub-batch division sequence and inspection order sequence; S4, generate a specific scheduling plan according to the sub-batch division sequence and inspection order sequence; S5, convert the scheduling plan into an operation instruction sequence for several sub-inspection machines in the equipment, so that the equipment in the equipment All inspection machines are inspected under unified scheduling. The invention calculates optimal sub-batch division through migratory bird migration algorithm, improves the flexibility of inspection, and improves the efficiency of batch inspection.

The invention relates to a reagent bottle bracket for a liquid suction system. The reagent bottle bracket comprises a bracket body; and one or more bottle cavities formed in the bracket body and suitable for accommodating one or more reagent bottles, wherein when the bracket body is horizontally placed, the one or more bottle cavities are inclined. According to the invention, the dead volume of liquid suction when automatic immunoassay equipment sucks liquid in a reagent bottle is effectively reduced, the utilization rate of the reagent is improved, and the experiment cost is saved. Meanwhile,the problem that the reagent bottles interfere with each other when the reagent bottles are replaced is solved, and the quality of reagents is guaranteed.