Full-automatic immunohistochemical staining instrument and staining method thereof

Abstract

The invention relates to a full-automatic immunohistochemical staining instrument and a staining method thereof.The full-automatic immunohistochemical staining instrument comprises a rack, a staining box assembly, a reagent bottle assembly, a movable sample adding needle, a universal reagent sample adding assembly and a waste liquid collecting box; the dyeing box assembly is installed on the rack and comprises a dyeing box and a driving mechanism used for driving the dyeing box to shake, the dyeing box is installed on the driving mechanism and comprises a container and a cover body, the two side walls of the container are provided with an opening used for installing a slide and a liquid discharging opening used for discharging liquid respectively, and the waste liquid collecting box is arranged on the liquid discharging opening side of the dyeing box; the reagent bottle assembly is installed on the rack and comprises a reagent bottle rack and reagent bottles, containing grooves used for installing the reagent bottles are evenly formed in the top of the reagent bottle rack, and the reagent bottles are used for containing different reagents; the contact efficiency of tissues and reagents is enhanced through a movement device, the reaction time of primary antibodies and secondary antibodies is shortened, and the device is suitable for further popularization and application.

Description

technical field [0001] The invention relates to the technical field of medical testing equipment, in particular to an automatic immunohistochemical staining instrument and a staining method thereof. Background technique [0002] Immunohistochemistry (referred to as immunohistochemistry) technology is the gold standard for solid tumor diagnosis, but there are still many deficiencies in this technology in tumor diagnosis: first, there are many processing steps, manual operation is error-prone, and the consistency is poor; the current automation The processing time of the instrument is long, the stability is not high, and it is impossible to perform rapid immunohistochemical staining on the sections, so as to quickly diagnose the case. At present, there is still a lot of room for improvement in the staining time of automatic immunohistochemical staining instruments. At present, one of the main reasons for the slow detection process of the instrument is that the same robotic ar...

AI Technical Summary

Problems solved by technology

[0002] Immunohistochemistry (referred to as immunohistochemistry) technology is the gold standard for solid tumor diagnosis, but there are still many deficiencies in this technology in tumor diagnosis: first, there are many processing steps, manual operation is error-prone, and the consistency is poor; the current automation The processing time of the instrument is long, the stability is not high, and it is impossible to perform fast immunohistochemical staining on the sections, so as to quickly diagnose the case

At present, one of the main reasons for the slow detection process of the instrument is that the same robotic arm moves in the way of X / Y / Z degrees of freedom to realize the addition of various liquid reagents, and the three-dimensional positioning takes a long time and moves slowly.

[0003] In addition, another main reason for the long staining process is the reaction of the antigen on the pathological tissue and the primary antibody; the traditional reaction is to add the primary antibody above the slide tissue and react statically under constant temperature conditions. The whole process takes 1 hour left and right, prolonging the immunohistochemical staining time

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