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Cleaning buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of cleaning buffer solution

A technology of immunohistochemistry and buffer solution, which is applied in the field of automatic immunohistochemistry staining machine, which can solve the problems of storage, transportation, and inconvenient use, and achieve long-term stable storage and use, increase safety, and maintain the effect of osmotic pressure

Pending Publication Date: 2022-06-07
浙江莱阅病理诊断科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since sodium azide is a well-known dangerous chemical reagent, there are many inconveniences in storage, transportation, and use. Therefore, it is extremely important to find alternative reagent formulations

Method used

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  • Cleaning buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of cleaning buffer solution
  • Cleaning buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of cleaning buffer solution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A washing buffer A: each 1000 mL of the washing buffer A includes: tris(hydroxymethyl)aminomethane (Tris) 11.0 g, sodium chloride 75.0 g, Tween-203.5 mL, ProClin 950 2.5 mL and the remainder of the purification Water, pH range is pH 7.3 to 7.5.

[0034] A cleaning buffer solution: each 1000 mL of 1× cleaning buffer includes: 100 mL of cleaning buffer solution A and 900 mL of purified water, and the pH value of the cleaning buffer solution ranges from pH 7.3 to 7.5.

[0035] Preparation method of washing buffer A: Weigh Tris according to the ratio, dissolve Tris in purified water, add sodium chloride in the formula amount, add Tween-20 after complete dissolution, add ProClin 950 after dissolution, and finally make up to volume with purified water To the rated capacity, use 1M NaOH solution or HCl solution to adjust the pH value of the cleaning buffer so that the pH value is in the range of pH 7.3 to 7.5.

[0036] The preparation method of the cleaning buffer in the pres...

Embodiment 2

[0038] A washing buffer solution A: every 1000 mL of the washing buffer solution A includes: tris(hydroxymethyl)aminomethane (Tris) 12.0 g, sodium chloride 82.0 g, Tween-204.5 mL, ProClin 9504.0 mL and the balance of purified water , the pH range is pH7.3~7.5.

[0039] A cleaning buffer: each 1000 mL of 1× cleaning buffer includes: 100 mL of cleaning buffer A and 900 mL of purified water, and the pH value is in the range of pH 7.3 to 7.5.

[0040] Preparation method of washing buffer A: Weigh Tris according to the ratio, dissolve Tris in purified water, add sodium chloride in the formula amount, add Tween-20 after complete dissolution, add ProClin 950 after dissolution, and finally make up to volume with purified water To the rated capacity, use 1M NaOH solution or HCl solution to adjust the pH value of the cleaning buffer so that the pH value is in the range of pH 7.3 to 7.5.

[0041] Preparation method of cleaning buffer: measure the volume of cleaning buffer A and purified...

Embodiment 3

[0043] A washing buffer solution A: every 1000 mL of washing buffer solution A includes: tris(hydroxymethyl)aminomethane (Tris) 12.5 g, sodium chloride 90.0 g, Tween-206.0 mL, ProClin 9505.0 mL and the balance of purified water , the pH range is pH7.3~7.5.

[0044] A cleaning buffer: each 1000 mL of 1× cleaning buffer includes: 100 mL of cleaning buffer A and 900 mL of purified water, and the pH value is in the range of pH 7.3 to 7.5.

[0045] Preparation method of washing buffer A: Weigh Tris according to the ratio, dissolve Tris in purified water, add sodium chloride in the formula amount, add Tween-20 after complete dissolution, add ProClin 950 after dissolution, and finally make up to volume with purified water To the rated capacity, use 1M NaOH solution or HCl solution to adjust the pH value of the cleaning buffer so that the pH value is in the range of pH 7.3 to 7.5.

[0046] Preparation method of cleaning buffer: measure the volume of cleaning buffer A and purified wat...

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Abstract

A cleaning buffer solution adapted to a full-automatic immunohistochemical staining machine and a preparation method thereof are characterized in that the cleaning buffer solution is formed by dissolving a prepared cleaning buffer solution A in purified water, the cleaning buffer solution A comprises the following main components: tris (hydroxymethyl) aminomethane, sodium chloride, Tween-20 and ProClin950, and the pH value range of the cleaning buffer solution A is 7.3-7.5. The technical scheme of the invention has the advantages that the use of sodium azide can be avoided, the consistency, stability and accuracy of the detection result are ensured, and the dyeing effect and the cleaning effect can be improved.

Description

technical field [0001] The application relates to the technical field of automatic immunohistochemical staining machines, in particular to a cleaning buffer suitable for automatic immunohistochemical staining machines and a preparation method thereof. [0002] technical background [0003] Immunohistochemical technology has developed rapidly in recent years, and with the application and promotion of automatic immunohistochemical staining instruments, the requirements for staining quality are also increasing. Among them, sample cleaning plays an important role in the entire immunohistochemical staining process, and the quality of the cleaning effect can also directly determine the quality of the final staining results. Because of its own structural characteristics, the automatic stainer has a small container for holding the washing buffer, and it cannot completely immerse the tissue sections in the washing buffer like manual washing of the sample, and the automatic immunoassay...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N1/30
CPCG01N33/531G01N1/30
Inventor 沈雯徐秀芬
Owner 浙江莱阅病理诊断科技有限公司
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