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Full-automatic immunohistochemical instrument cover glass washing method

A technology of immunohistochemistry and washing methods, which is applied in the direction of chemical instruments and methods, cleaning methods using liquids, cleaning methods using tools, etc., and can solve problems affecting the circulation and diffusion of reagent liquid surfaces, increasing dirt and thickening, and diagnostic errors, etc. problems, to achieve the effects of complete fading, improved dyeing quality, and extended service life

Active Publication Date: 2020-07-24
SHANGHAI BAOSHAN DISTRICT RENHE HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during use, it was found that the coverslip would be stained brown by the DAB chromogenic agent (Zheng Jun, Zhang Gu, He Xiaoyan, Luo Likang. In the chromogenic solution, 3,3'-diaminobenzidine and H 2 o 2 The effect of content on immunohistochemical antigen detection [J]. Modern Practical Medicine, 2009, 21 (12) 1315-1316) and the phenomenon of increased dirt and thickening during staining, according to the recommended washing scheme in the instructions of the automatic immunohistochemical instrument It is difficult to completely remove, and requires the cover slip to be replaced if it is seriously discolored or damaged, which will affect the circulation and diffusion of the reagent liquid surface in the next use, so that the reagent cannot be evenly spread on the slide containing the tissue section to be tested, and local or False negatives of regional chromogenic failure directly lead to diagnostic difficulties or diagnostic errors, such as expensive discarding

Method used

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  • Full-automatic immunohistochemical instrument cover glass washing method
  • Full-automatic immunohistochemical instrument cover glass washing method
  • Full-automatic immunohistochemical instrument cover glass washing method

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Reagent preparation 1: Potassium permanganate 5g, distilled water 500mL, make 1% potassium permanganate solution 500mL.

[0041]Reagent preparation 2: 5 g of sodium metabisulfite, 500 mL of distilled water, and 500 mL of 1% sodium metabisulfite solution.

[0042] Reagent preparation 3, Tween_20 15mL, distilled water 985mL, dubbed 1.5% Tween_20 solution 1000mL.

[0043] Method: After the immunohistochemical detection by the automatic immunohistochemical instrument, the used coverslips were rinsed with tap water to wash off the color and dirt easily, soaked in 500mL distilled water for 1 min, drained until no water dripped, and then put in 1 % Potassium permanganate solution 500mL, let stand for 1h, rinse with tap water until the color of potassium permanganate solution disappears, then rinse with water for 30s, put in 500mL of distilled water for 1min, drain until no dripping, put in 500mL of 1% sodium metabisulfite solution to stand still Put it in place for 20 minutes...

Embodiment 2

[0045] Reagent preparation 1, potassium permanganate 2.5g, distilled water 500mL, made into 0.5% potassium permanganate solution 500mL.

[0046] Reagent preparation 2, sodium metabisulfite 2.5g, distilled water 500mL, made into 0.5% sodium metabisulfite solution 500mL.

[0047] Reagent preparation 3, Tween_20 10mL, distilled water 990mL, made 1% Tween_20 solution 1000mL.

[0048] Method: After the immunohistochemical detection by the automatic immunohistochemical instrument, the used coverslips were rinsed with tap water to wash off the color and dirt easily, soaked in 500mL distilled water for 1min, drained until no water dripped, and then put in 0.5 % Potassium permanganate solution 500mL, let stand for 1h, rinse with tap water until the color of potassium permanganate solution disappears, then rinse with water for 30s, put in 500mL of distilled water for 1min, drain until no dripping, put in 500mL of 0.5% sodium metabisulfite solution to stand still Put it in place for 5 m...

Embodiment 3

[0050] Reagent preparation 1, potassium permanganate 25g, distilled water 500mL, dubbed 5% potassium permanganate solution 500mL.

[0051] Reagent preparation 2, sodium metabisulfite 10g, distilled water 500mL, made into 2% sodium metabisulfite solution 500mL.

[0052] Reagent preparation 3, Tween_20 100mL, distilled water 900mL, made 10% Tween_20 solution 1000mL.

[0053] Method: After the immunohistochemical detection by the automatic immunohistochemical instrument, the used coverslips were rinsed with tap water to wash off the color and dirt easily, soaked in 500mL distilled water for 1min, drained until no water dripped, and put in 5 % Potassium permanganate solution 500mL, let stand for 1h, rinse with tap water until the color of potassium permanganate solution disappears, then rinse with water for 30s, put in 500mL of distilled water and soak for 1min, drain until no dripping, put in 500mL of 2% sodium metabisulfite solution to stand still Put it in place for 30 minutes...

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Abstract

The invention discloses a full-automatic immunohistochemical instrument cover glass washing method. The full-automatic immunohistochemical instrument cover glass washing method comprises the followingsteps that after the surface of cover glass obtained after immunohistochemical detection through a full-automatic immunohistochemical instrument is thoroughly flushed by tap water, a potassium permanganate solution with the concentration being 0.5-5%, a sodium metabisulfite solution with the concentration being 0.5-2% and a Tween_20 solution with the concentration being 1-10% are sequentially used for flushing, and the thoroughly-cleaned full-automatic immunohistochemical instrument cover glass is obtained. The disclosed full-automatic immunohistochemical instrument cover glass washing methodhas the advantages that fading is thorough, the dirt removing capability is high, the service life of the cover glass is prolonged, and cost is saved.

Description

technical field [0001] The invention belongs to the technical field of coverslip cleaning, in particular to a method for washing coverslips of an automatic immunohistochemical instrument. Background technique [0002] Cover slips are the consumables required for staining in automatic immunohistochemical instruments, which can well solve the unevenness of reagent dripping (Ding Wei, Wang Detian. Concise Pathological Technology [M]. Hangzhou: Zhejiang Science and Technology Publishing Society, 2014:33, 158-163), especially suitable for the situation where the reagents cannot be completely covered after the reagent is dropped due to too large tissue sections or scattered distribution. However, during use, it was found that the coverslip would be stained brown by the DAB chromogenic agent (Zheng Jun, Zhang Gu, He Xiaoyan, Luo Likang. In the chromogenic solution, 3,3'-diaminobenzidine and H 2 o 2 The effect of content on immunohistochemical antigen detection [J]. Modern Practic...

Claims

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Application Information

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IPC IPC(8): B08B1/00B08B3/02B08B3/04B08B3/08B08B3/12B08B13/00
CPCB08B3/02B08B3/04B08B3/08B08B3/12B08B13/00B08B1/12
Inventor 陈荣范宜娟杨滢瑜潘晓燕汤芃芃王周璁
Owner SHANGHAI BAOSHAN DISTRICT RENHE HOSPITAL
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