41 results about "Antiviral gene" patented technology

The invention relates to a reagent box, in particular to an anti-virus gene MxA fluorescence quantitative reagent box and the detection method thereof. The reagent box comprises MxA, housekeeping gene GAPDH amplification primers, DNA polymerase a probe used for PCR reaction, and one or more kind(s) of buffer solution thereof. The detection method comprises the following steps: firstly, peripheral blood mononuclear cell PBMC is gathered; secondly, PBMC cells with interferon are irritated; thirdly, a TA clone including MxA and housekeeping gene GAPDH target gene fragments is established; fourthly, real-time fluorescence PCR is adopted to detect the inducible expression of MxA mRNA; fiftyly, the Ct value of samples is judged according to the standard curve and the domain value. The anti-virus gene MxA fluorescence quantitative reagent box and the detection method of the invention have the advantages that after adopting the irritation of the peripheral blood interferon, the expression of the MxA can reflect the therapeutic effect of patients with interferon after being treated, thus patients and doctors have vital significance to select the interferon treatment, and the waste of medical resources is avoided.

The invention relates to a recombinant HSV (Herpes Simplex Virus) amplicon vector and application thereof. By using two replication deficient adenoviruses respectively carrying Cre and an operative linked component loxP-HSVoriS-pac-transgenic expression box-loxP, a novel HSV amplicon vector is recombined in a coinfection cell. Being different from the traditional HSV amplicon vector taking bacterial plasmids as a skeleton, the amplicon vector does not contain a bacteria copying sequence (colEorigin) and a resistance gene component and only contains oriS of HSV, a pac sequence and a transgenic expression box. The recombinant HSV amplicon vector disclosed by the invention is used for preparing a novel HSV amplicon vector, which does not contain the bacterial gene component and is used for various types of transgenic researches and tumour gene treatments, and preparing an adenovirus treatment preparation for specific anti-HSV virus and related diseases. The HSV amplicon vector recombined by the replication deficient adenovirus of the preparation in cells replicates itself by using infected wild HSV virus and competitively inhibits or permanently expresses the antiviral genes so as to inhibit replication of wild HSV virus. Therefore, the recombinant HSV amplicon vector can be used for resisting HSV infection and treating related diseases thereof.

The invention relates to the isolation and the cloning and breeding application of a tobacco mosaic virus (TMV) resistant N'au gene. The invention discloses the nucleotide sequence of the TMV resistant N'au gene shown as SEQ ID NO.1. The amino acids of polypeptide encoded by the TMV resistant N'au gene are shown as SEQ ID NO.2. A non-transgenic TMV resistant tobacco variety can be obtained by transferring an N'au gene which is contained by germplasm resources into a TMV infected popular tobacco variety by conventional breeding means. The N'au gene of the invention has a homologous sequence with high identity rate of nucleotides in the popular tobacco variety, so that it is easy to obtain a shorter introgression segment single plant carrying the N'au gene by conventional breeding, and thereby to obtain a TMV resistant variety with lower linkage drag. The gene can resist both U1 strain and Cg strain of TMV. The novel antiviral gene N'au of the invention has great application prospect in cultivation of a TMV resistant tobacco.

The invention relates to the field of anti-virus genes, and particularly discloses application of a duck BCL2L15 gene in livestock and poultry for resisting an avian influenza virus (AIV). The invention provides the new gene BCL2L15 relevant to resisting of avian influenza, and it is verified that the BCL2L15 has the effect of restraining replication of the AIV by utilizing a method of combining molecular biology with cell biology experiments fir the first time. It is shown through analysis that compared with a cell which is not subjected to overexpression of the BCL2L15 gene, the cell subjected to overexpression of the BCL2L15 gene can significantly restrain replication of the AIV, therefore, in-depth function study can be conducted on the duck BCL2L15 gene, and a key protein structure or amino acid of the duck BCL2L15 gene for resisting the AIV is determined. By means of gene editing methods such as the transgenic technology, transgenic livestock and poultry which inducibly and highly express the BCL2L15 gene of ducks or other animals are obtained, and good varieties of transgenic farm animals capable of resisting various viruses such as the AIV are bred.

The invention relates to a method for inhibiting cellular expression of Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4. The invention relates to the field of biological medicine, and in particular relates to a method for inhibiting the cellular expression of antiviral gene cells and the method is screened by measuring the expression quantities of Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 and by constructing a vesicular stomatitis virus susceptible cell model caused by overexpression of RXRs (retinoid X receptors). The invention provides a constructing method of the vesicular stomatitis virus susceptible cell model caused by the overexpression of the RXRs, and applications of the RXRs in screening the method for inhibiting the cellular expression of the Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4, and finds that a COX sensing pathway inhibits antiviral genes in a host immune defense system through an RXR-dependent manner, and therefore, the practical application value of the RXRs in screening the method for inhibiting the cellular expression of the Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 is further confirmed.

The invention especially relates to application of RXR in screening of agonists of Ifnbeta, Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4, which belongs to the field of biological medicine. The invention provides application of RXR in screening of agonists of Ifnbeta, Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 and a construction method for a susceptible cell model representing down-regulated expression of antiviral genes like Ifnbeta, Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 caused by overexpression of RXR. It is found that a COX susception pathway exerts inhibitory effect on antiviral genes in a host immune defense system in an RXR-dependent manner. Application of RXR in screening of the agonists of Ifnbeta, Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 is further expounded.

The invention relates to the field of plant antivirus genetic engineering, and discloses an attenuated vaccine against potato X virus, a preparation method and application thereof. The attenuated vaccine against Potato virus X is based on the TVBMV attenuated mutant, and an effective gene fragment that can induce cross-protection to Potato virus X is embedded in the TVBMV attenuated mutant, and the effective gene fragment includes at least the RdRp gene of Potato virus X . The RdRp gene includes Rd1 gene, Rd2 gene and Rd3 gene, and one of Rd1 gene, Rd2 gene and Rd3 gene is embedded in the attenuated TVBMV mutant. The anti-potato virus X virus attenuated vaccine of the present invention has a stable effect, can play an effective cross-protective role, significantly reduces the damage of plants infected by the potato X virus virulent strain, delays the onset of plants, and greatly reduces losses.

The invention relates to clone, reconstruction and transformation of a precursor (pre-miR159a) of arabidopsis thaliana microRNA159a and antivirus analysis of a transgenic plant and belongs to the techThe invention relates to clone, reconstruction and transformation of a precursor (pre-miR159a) of arabidopsis thaliana microRNA159a and antivirus analysis of a transgenic plant and belongs to the technical field of molecular biology and biology. The technology clones a nucleotide sequence of pre-miR159a from the arabidopsis thaliana and carries out site-directed mutagenesis on the sequence producinical field of molecular biology and biology. The technology clones a nucleotide sequence of pre-miR159a from the arabidopsis thaliana and carries out site-directed mutagenesis on the sequence producing mature miR159a. 126kDa protein of tobacco mosaic virus, 25kDa protein of potato virus X and HC-Pro protein of potato virus Y are subjected to genetic mutation to obtain three sections of the mutating mature miR159a. 126kDa protein of tobacco mosaic virus, 25kDa protein of potato virus X and HC-Pro protein of potato virus Y are subjected to genetic mutation to obtain three sections of the mutation sequences. The three sections of the sequences are in series to construct the plant expression vector; and an agrobacterium-mediated method is adopted to transform nicotiana benthamiana to obtain ton sequences. The three sections of the sequences are in series to construct the plant expression vector; and an agrobacterium-mediated method is adopted to transform nicotiana benthamiana to obtain transgenic tobacco. The transgenic plant has high disease resistance on three viruses. The antivirus strategy has the advantages of strong disease resistance, durable disease resistance, biological safransgenic tobacco. The transgenic plant has high disease resistance on three viruses. The antivirus strategy has the advantages of strong disease resistance, durable disease resistance, biological safety and the like and has wide application prospect in the breeding field of plant antivirus genetic engineering.ety and the like and has wide application prospect in the breeding field of plant antivirus genetic engineering.

The invention relates to a set of primers for amplifying classic locus sequences of MHC class I genes of crested ibis and a genotyping method, wherein the sequences of the primers are as shown in SEQ ID NO. 1-SEQ ID NO. 10. By virtue of the amplification primers disclosed by the invention, the nucleotide sequences of various class loci of class I MHC of the crested ibis are effectively amplified, and each individual is subjected to genotyping by virtue of subsequent SSCP-HD (single-strand conformation polymorphism-HD) or sequencing technology. The specific primers disclosed by the invention have the advantages of being strong in specificity, and simple and rapid in operating process; and by typing the classic loci of the class I MHC genes of the crested ibis, the polymorphism levels of the classic loci of the class I MHC genes of the crested ibis individual are assessed and the potential of the crested ibis in resisting viral diseases is detected, so that an important reference is provided for such operations of new population founder selection, reintroduction individual selection, artificial mating selection and the like in crested ibis protection work.

The invention relates to the field of plant anti-virus genetic engineering, and discloses a tobacco mosaic virus gene fragment for efficiently producing siRNA, an attenuated vaccine, a preparation method and an application thereof. The tobacco mosaic virus gene fragments that efficiently produce siRNA include at least one of the TMV1 fragment, the TMV2 fragment and the TMV3 fragment, and the nucleotide sequences of the TMV1 fragment, the TMV2 fragment and the TMV3 fragment are respectively Seq ID No.13, Seq ID No. 14. As shown in Seq ID No.15, the gene fragment can efficiently produce siRNA after being inoculated with parasitic plants. The attenuated vaccine is based on the attenuated TVBMV mutant, and the TVBMV attenuated mutant is embedded with an effective gene fragment that can induce cross-protection against tobacco mosaic virus, and the effective gene fragment includes a tobacco mosaic virus gene fragment that can produce siRNA. The anti-tobacco mosaic virus attenuated vaccine of the present invention has a stable effect, can play an effective cross-protection role, significantly reduces the damage of plants infected by strong tobacco mosaic virus strains, delays the onset of plants, and greatly reduces losses.

The invention discloses a ceratopteris pteridoides gene for resisting human immunologic deficiency disease toxoprotein congeners, and a preparation method and application thereof. The preparation method comprises the following steps: 1, extracting total DNA by using an improved CTAB method, and preserving; 2, amplifying the intermediate segment of the ceratopteris pteridoides gene for resisting human immunologic deficiency disease toxoprotein congeners; 3, amplifying 5' flanking sequence of the ceratopteris pteridoides gene for resisting human immunologic deficiency disease toxoprotein congeners; 4, amplifying 3' flanking sequence of the ceratopteris pteridoides gene for resisting human immunologic deficiency disease toxoprotein congeners; and 5, recycling and cloning the PCR product: recycling the intermediate segment of the target gene, and 5' and3' flanking sequence segments, transforming, culturing and selecting white colonies; and 6, judging the clone, measuring the sequence and splicing. The gene can be used for regulation and control on expression of potential antivirus genes in plant research, carrying out research into plant antivirus gene engineering, and further establishes a ceratopteris pteridoides CVNH protein preparation system, thereby finally realizing large-scale industrial production.

The invention belongs to the technical field of biology, and relates to a disease resistance detection of a plant antiviral gene and an application thereof. According to the invention, a soybean endogenous antiviral gene Gm-RSMV3 is used to construct a transformation vector which is used for transforming sensitive varieties of soybean mosaic virus; and the transformed plants are subjected to herbicide resistance screening, and then subjected to genome DNA PCR, bar gene expression detection, RT-PCR, Southernblot and inverse PCR, thereby verifying that the obtained plants are indeed transgenic and exogenous genes are correctly expressed. T0 generation plants are selfed to obtain T1 generation plants which are also selfed to obtain T2 generation homozygous plants, and the obtained T2 generation homozygous plants are inoculated with the soybean mosaic virus. A disease resistance testing is carried out and results show that transgenic plants of varieties being originally not resistant to the soybean mosaic virus are relatively high in disease resistance. Conventional cross-breeding and transgenic technologies can be used for enhancing disease-resistance performance of plants and increasing production.

The invention discloses a tobacco curly shoot virus satellite promoter. The promoter comes from an alpha satellite molecule accompanying with the tobacco curly shoot virus. The separated promoter is an upstream noncoding region sequence of a RepORF of the alpha satellite molecule. The invention relates to a promoter sequence of the RepORF of the alpha satellite molecule accompanying with the tobacco curly shoot virus and an expression cassette of the RepORF promoter sequence. The promoter disclosed by the invention can be applied to plant anti-virus gene engineering and plant transgenic engineering.