32 results about "Acyrthosiphon pisum" patented technology

The invention relates to an artificial mass propagation method of harmonia axyridis. Two facilities of a seedling raising greenhouse and an insectary are adopted, broad beans are used as host plants for propagating pea aphids which are used as aphids for feeding the harmonia axyridis, and the harmonia axyridis which is used for agricultural control over the aphids can be manually propagated in a large quantity through specific technical measures of broad bean plant breeding, pea aphid inoculation and propagation, artificial breeding of the harmonia axyridis and the like. Healthy broad bean sprouts are bred with broad bean seeds, the pea aphids are inoculated with the broad bean sprouts and propagated, then the pea aphids are collected to feed larvas and adults of the harmonia axyridis, and a large quantity of the larvas and the adults of the harmonia axyridis are propagated to be released in agricultural production areas to control the aphids of all kinds, so that the biological control over the aphids is realized, and the pollution and safety problems caused by chemical control is avoided or reduced.

The invention discloses a method for determining influence of exogenous dsRNA on toxicity of ladybugs. The method comprises the following specific steps: uniformly mixing the exogenous dsRNA into a sucrose solution, directly feeding the ladybugs for 2-3 days and then feeding with pea aphids; then detecting and analyzing the expression changes of target genes in the ladybugs, and observing the biological changes of the ladybugs so as to evaluate the toxicity of the exogenous dsRNA to the ladybugs, wherein the ladybugs include harmonia axyridis, coccinella septempunctata or henosepilachna pusillanima. By the method, direct influence of the exogenous dsRNA on the toxicity of the harmonia axyridis, the coccinella septempunctata or the henosepilachna pusillanima or other ladybugs can be effectively determined; in addition, the method is simple, feasible and high in effectiveness and sensitivity, and has a great significance and an application prospect in study on functions of related genesas well as environmental risk assessment on related RNAi transgenic crops.

The invention provides a method for propagating Aphidius gifuensis by pea aphids. The method provided by the invention utilizes two facilities including a seedling culture big shed and a field small shed and takes broad beans and peas as host plants for propagating aphids; the method takes the pea aphids as host aphids for propagating Aphidius gifuensis and then the propagated Aphidius gifuensis are released to a large-scale tobacco field to kill the aphids on tobacco leaves. The method selects the broad bean seeds and the pea seeds and then tender green bean plants are cultivated in a big greenhouse or the small shed built in the fields; the pea aphids are inoculated on the bean plants and then the pea aphids are propagated; then the Aphidius gifuensis is inoculated on the tender bean plants which is propagated with a lot of pea aphids and then the Aphidius gifuensis is propagated. Then a lot of propagated Aphidius gifuensis are released into the large-scale tobacco field to kill theaphids on tobacco leaves. Finally, the aim of biologically preventing and treating aphids is realized and the pesticide pollution caused by preventing the aphids is avoided.

The invention discloses an artificial reproduction technological process and a method of application of aphidoletes aphidimyza. An aphidoletes aphidimyza mass reproduction technology and an application technology are characterized in that pea aphids, pea repaired tail aphids and aphis craccivora koch are reproduced massively by adopting broad beans, and the aphidoletes aphidimyza is reproduced by utilizing the pea aphids, the pea repaired tail aphids and the aphis craccivora koch. The aphidoletes aphidimyza mass reproduction technology and the application technology comprise the following processes: net seedlings are planted, aphids are raised, the aphidoletes aphidimyza is raised, old larvae are collected, and the larvae are saved in the pupal period. Adults are applied to field application and expanding reproduction after eclosion. An efficient and superior method technological process for reproducing aphidoletes aphidimyza is developed so as to solve the to-be-solved problems, and the method that the aphidoletes aphidimyza is used to prevent and control aphids can play a proper role in safe production of vegetables.

The invention discloses a harmonia axyridia larva indoor industrialized culturing method. The method comprises the steps that eggs which are stored in 1-4 days from a cold storage, the eggs are gathered and placed in a hatching box to be hatched, initially hatched larvae are placed in culturing boxes to be cultured; a layer of filter paper is laid at the bottom of each of the culturing boxes, andthe culturing boxes are internally provided with silk-shaped filler to expand the moving range of the larvae; sufficient acyrthosiphon pisum is regularly placed in each culturing box, and horsebean seedlings with cut roots are fixedly placed until the larvae become chrysalides; the feeding of the acyrthosiphon pisum to the harmonia axyridia which becomes a chrysalis is stopped, and the emergence harmonia axyridia is cultured in an insect culturing cage for production. According to the method, industrialized culturing of the harmonia axyridia in the whole developing period is achieved to ensurethe uniformity of the hatching time, the pupation time and the emergence time, convenience is provided for manual concentration management culturing, the killing rate among the harmonia axyridia in the harmonia axyridia larva period is reduced, the duration of egg production of the harmonia axyridia is improved to obtain the adult harmonia axyridia with uniform developing duration and high-yieldeggs, and time and space of industrialized harmonia axyridia propagation are saved.

The invention provides a biological assaying method for evaluating the effect of transgene cotton on chrysopa pallens. The method comprises the following steps: paving agar on the bottom of a culture dish; placing transgene cotton leaves on the surface of the agar, inoculating newly-hatched helicoverpa armigera larvae; feeding newly-hatched chrysopa pallens larvae for 1 to 3 days with pea aphid, then feeding the chrysopa pallens larvae with the helicoverpa armigera larvae which has been fed by the transgene cotton leaves for 1 to 3 days; observing the growth situation of the chrysopa pallens larvae since the first day when the chrysopa pallens larvae is fed with the helicoverpa armigera larvae until the chrysopa pallens larvae pupate and perform eclosion; setting a reference group; comparing the differences of the experiment group and the reference group on the indexes of pupation rate, eclosion rate, larva weight, imago weight, and total growth time, and analyzing the difference significance through variance analysis so as to evaluate the effect of transgene cotton on chrysopa pallens. The application range of the biological assaying method is wide. The effect of high dose protein in transgene plants on predators can be effectively measured by utilizing the nutrition relationship evaluation of transgene plant-pest-predator.

The invention belongs to the field of green pest insect prevention and control, relates to a method for artificially breading great amounts of pea aphids. The method includes the following steps of a, processing seeds, b, filling substrate and sowing, c, inputting a floating disc into water, d, seedling vaccination, e, aphid stock, f, seedling shearing and aphid feeding, g, later-period processing and h circulating. The method for artificially breading the pea aphids relates to the artificial expanding breeding method of living body food insects, and the method is suitable for artificial feeding of natural enemy insects.

The invention discloses a rapid identification method for broad bean resistance to Acyrthosiphon pisum. The rapid identification method includes: selecting full broad bean seeds, using clean water to clean the broad bean seeds, soaking the broad bean seeds till the broad bean seeds completely expand; evenly arranging the broad bean seeds in a tray, cultivating the broad bean seeds in a lighting-dark alternative condition, and regularly observing the growth condition of broad bean seedlings; and putting 2-3 Acyrthosiphon pisum without wings on a front surfaces of each leaf while each broad bean seedling has 2-3 leaves, and observing the susceptibility of the broad bean seedlings. Growth and reproduction of the Acyrthosiphon pisum is affected by seasons, the environment condition of a cultivation room is stable, and the identification system is a complete closed system, and in this way, the repeatability and the reliability of the identification result can be ensured.

The present invention relates to a method for artificial propagation of pea aphids, comprising the following steps: a. seed treatment; b. filling matrix for sowing; c. floating tray into water; d. emergence inoculation; Feeding; g. Post-treatment; h. Cycle mode. The invention relates to a method for artificial propagation of pea aphids, which belongs to the field of green prevention and control of pests, relates to a method for artificial propagation of a large number of living food insects, and is suitable for artificial breeding of natural enemy insects.

The invention relates to an indoor artificial holidic diet and raising method for acythosiphon pisum harris. Components (mainly comprising an amino acid stock solution, a vitamin stock solution, a mineral stock solution and a cane sugar mixed solution), the proportion, and the preparation method of the artificial holidic diet for the acythosiphon pisum harris, and the raising method of the acythosiphon pisum harris, the raising conditions of the acythosiphon pisum harris, and the like are comprehensively researched, so that the weight, the propagation rate, and the reproductivity of raised acythosiphon pisum harris are notably improved, and requirements for large-scale raising of the acythosiphon pisum harris can be met.

The invention discloses a method for evaluating the influence of insecticidal protein on adults of propylaea japonica and a feeding device. The invention firstly designs a feeding device for simulating the plant gland, and the suction mode of cotton flower extrafloral nectaries is simulated, and then a cotton juice extract is used as a solvent to dissolve the exogenous insecticidal protein, and fructose is added to serve as a phagostimulant of the propylaea japonica adults; a single-pair of the propylaea japonica adults are put into the feeding device with a feeding solution, and enough pea aphids are supplemented to culture in an illumination cultivation box. According to the indexes of the to-be-detected solution on the death rate of the propylaea japonica adults, the egg laying early stage, the egg laying amount and the weight, whether the insecticidal protein has the influence on the propylaea japonica adults or not is judged. On the base of establishing the risk theory of evaluating that the secondary metabolites and the insecticidal protein in the plant interact to influence the propylaea javanica, the natural environment of the insecticidal protein threat which is faced by the propylaea ladyridis can be reflected more truly, the evaluation method is simple, and the applicability is high.

The present invention relates to a simple and rapid insect host plant culture technology, which is characterized in that an insect host plant is subjected to rapid water culture under a soilless condition. According to the present invention, the method has characteristics of space saving, cost saving, easy operation, and seed soil-borne disease occurrence reducing; and with the technology, the simple and low-cost method is provided for large-scale culture of insect host plants, the work efficiency is improved, and the strong practicality is provided.

The invention discloses a low-density breeding method capable of maintaining a wingless state of acyrthosiphon pisum viviparous female. The low-density breeding method includes the steps of planting of broad beans, cultivation of aphids, group experiments, observation and data recording. The low-density breeding method has the advantages of simple operation, stable state, no seasoning restrictionand the like, materials for studying a wing-shaped differentiation mechanism of aphids are provided, and a good foundation is laid for studying the aphid pest control.

The invention discloses a preparation method of an RNA interference sequence of a trehalase gene of Acyrthosiphon pisum. The method comprises the following steps: 1. selecting a target region of RNAiaccording to a trehalase sequence of the Acyrthosiphon pisum (XM_003245847.3), and using Primer5.0 to design a PCR amplification primer in the target region; 2. extracting the total RNA of the Acyrthosiphon pisum and obtaining a cDNA by reverse transcription, and then conducting PCR-amplification on the trehalase gene contained in the cDNA by using the amplification primer to obtain the target gene; and 3. amplifying a double-stranded DNA of interest containing a T7promoter sequence, and then synthesizing a double-stranded RNA using the kit.

The invention relates to a breeding device and method for larvae of harmonia axyridis. The device specifically comprises a culture dish, a culture dish cover and a physical barrier device; the physical barrier device is placed in the culture dish and provided with an insect passing mechanism; the method comprises the steps of establishing a pea aphid population, breeding adult insects of harmoniaaxyridis with pea aphids, collecting eggs of harmonia axyridis, monitoring hatching of the eggs, newly adding aphids every day and breeding newly-hatched larvae until pupation. By means of the breeding device and method for the larvae of harmonia axyridis, the occupied space is small, intensive breeding of the larvae of harmonia axyridis is facilitated, and the breeding device and method are suitable for high-density larva breeding; the gathering degree and meeting possibility of the larvae are reduced in the breeding process, the autotomy rate is reduced accordingly, and the yield of productsof harmonia axyridis is increased; through intensive breeding, the production cost is reduced, the efficiency is improved, and the application and popularization of harmonia axyridis in biological prevention and control are promoted.

The invention relates to the technical field of pest control and genetic engineering, and discloses a novel aphid control agent. The novel aphid control agent comprises dsRNA of aphids and beauveria bassiana spores. The invention further discloses a method for controlling aphids through combined application of RNAi and beauveria bassiana. The novel aphid control agent is used for treating plants to prevent and control aphids. According to the invention, the novel aphid control agent is developed by combined application of biocontrol fungi and RNAi technology, and the aphid control agent has aremarkable synergistic mechanism, can overcome defects in controlling aphids by using fungi or the RNAi technology alone, and shows aphid control capability of up to 80%. The novel aphid control agentcan be directly sprayed on crops in a field so as to effectively prevent and treat aphids. The method is wide in application range; corresponding dsRNAs and spore suspension liquid of beauveria bassiana can be mixed together for spraying treatment only by synthesizing dsRNAs of different target genes of aphids; and the method can be widely applied to aphids such as pea aphids, peach aphids and brown citrus aphids.

The invention provides a purification and expanding propagation method for propylaea japonica. The method comprises the steps that 1, the propylaea japonica is fed in an insect feeding cage, wherein horsebean seedlings are planted and enough pea aphids are fed in the insect feeding cage, a flowerpot of horsebean seedling planting is wrapped with a preservative film, and the number of the stretching blades of the horsebean seedlings is as little as possible so that most of spawn is spawned on the preservative film; 2, the spawned preservative film is collected, and larvae are hatched in a warm and humid condition; 3, the larvae are taken out in time and put into another insect feeding cage to be fed, after the larvae pupate, the spawn collecting operation in the step 2 is stopped; 4, after the spawn in another insect feeding cage in the step 3 is collected through the preservative film, the step 2, the step 3 and the step 4 are repeated until five generations, and the purified spawn is obtained; 5, after the purified spawn is hatched, expanding propagation in the insect feeding cage is conducted. The purification and expanding propagation method is beneficial to purify the generation number of propylaea japonica in the field and reduce newly hatched larvae and imagoes feeding of the spawn indoors, the operation is easy, the cost is low, and purification and expanding propagation for propylaea japonica indoors can be completed effectively.

The invention discloses a method, a primer and a kit for detecting blue light phototaxis of aphids and relates to the field of agriculture biotechnology. The method for detecting the blue light phototaxis of the aphids disclosed by the invention comprises the following steps: comparing amino acid sequences of CRY1 proteins of target aphids with amino acid sequences of CRY1 proteins of pea aphids; and determining the blue light phototaxis of the target aphids according to sequence comparing results. The detection method is short in time and high in accuracy; the defects such as heavy workload, long time and high possibility of influence of environment factors and the like of the conventional method for detecting phototaxis are avoided; the method is taken as significant technical renovation; the efficiency is greatly improved; the key technical support is provided for early warning and green control of the aphids; the method has an extremely important application value in the field of control of the aphids and can be applied to the fields of control of the aphids, prediction of aphid situation or early warning of the aphids.

The invention provides a purification and expanding propagation method for propylaea japonica. The method comprises the steps that 1, the propylaea japonica is fed in an insect feeding cage, wherein horsebean seedlings are planted and enough pea aphids are fed in the insect feeding cage, a flowerpot of horsebean seedling planting is wrapped with a preservative film, and the number of the stretching blades of the horsebean seedlings is as little as possible so that most of spawn is spawned on the preservative film; 2, the spawned preservative film is collected, and larvae are hatched in a warm and humid condition; 3, the larvae are taken out in time and put into another insect feeding cage to be fed, after the larvae pupate, the spawn collecting operation in the step 2 is stopped; 4, after the spawn in another insect feeding cage in the step 3 is collected through the preservative film, the step 2, the step 3 and the step 4 are repeated until five generations, and the purified spawn is obtained; 5, after the purified spawn is hatched, expanding propagation in the insect feeding cage is conducted. The purification and expanding propagation method is beneficial to purify the generation number of propylaea japonica in the field and reduce newly hatched larvae and imagoes feeding of the spawn indoors, the operation is easy, the cost is low, and purification and expanding propagation for propylaea japonica indoors can be completed effectively.

The invention discloses a preparation method of an insecticide for preventing and treating pea aphids. The preparation method comprises the following steps: drying the overground parts of Achnatheruminebrians in the shade, cutting the overground parts short, pulverizing the cut overground parts, adding distilled water into a sample, carrying out soaking, and performing juicing to obtain an extract liquid. With the insecticide, the effective mortality of red pea aphids can reach 24.24% in the first day and can reach 100% in the seventh day; the effective mortality of green pea aphids can reach25.23% in the first day and can reach 97.17% in the seventh day; the average aphid yield of red and green pea aphid treatment groups is obviously lower than the average aphid yield of a control group; the harm of propagation of a pea aphid population can be effectively inhibited; the preparation method is simple and convenient in process, low in cost, and short in required time; and the preparedinsecticide is good in insecticidal effect, convenient to operate and high in practicability.

The invention discloses an acyrthosiphon pisim CYP6A13 promoter and activity analysis, and belongs to the technical field of biological engineering. The invention provides an obtained acyrthosiphon pisim CYP6A13 promoter sequence which is represented by SEQ ID NO: 1; the regions from -1bp to -821bp of the SEQ ID NO: 1 represent a DNA sequence of the acyrthosiphon pisim CYP6A13 promoter; the regions from +1bp to +349bp of the SEQ ID NO: 1 represent a DNA sequence of a 5' non-translating region of a gene CYP6A13 of acyrthosiphon pisim P450. The acyrthosiphon pisim CYP6A13 promoter can be used for high-efficiency expression of a eukaryotic gene and is applied to a research on acquisition of low-abundance genes, so that high-level and stable expression can be obtained; an active significance is realized for the research on a target function.

The invention relates to the technical fields of pest control and genetic engineering, and discloses an aphid control agent: dsRNA comprising aphids and Beauveria bassiana spores; and also discloses a method for controlling aphids by combining RNAi and Beauveria bassiana, The plant is treated with the aphid control agent to prevent and control aphids. In the present invention, a new type of aphid control agent is developed through the combined application of biocontrol fungi and RNAi technology. The aphid control agent has a significant synergistic mechanism, can overcome the defects of using fungi or RNAi technology alone to control aphids, and can control aphids. 80%. The aphid control agent can be used to effectively prevent and control aphids by directly spraying crops in the field. The invention has a wide range of applications. It only needs to synthesize dsRNAs of different target genes of aphids, and then the corresponding dsRNAs can be mixed with the spore suspension of Beauveria bassiana and sprayed together. The method can be widely used in pea aphids and peach aphids and aphids such as the brown orange aphid.

The invention discloses a method, a primer and a kit for detecting blue light phototaxis of aphids and relates to the field of agriculture biotechnology. The method for detecting the blue light phototaxis of the aphids disclosed by the invention comprises the following steps: comparing amino acid sequences of CRY1 proteins of target aphids with amino acid sequences of CRY1 proteins of pea aphids; and determining the blue light phototaxis of the target aphids according to sequence comparing results. The detection method is short in time and high in accuracy; the defects such as heavy workload, long time and high possibility of influence of environment factors and the like of the conventional method for detecting phototaxis are avoided; the method is taken as significant technical renovation; the efficiency is greatly improved; the key technical support is provided for early warning and green control of the aphids; the method has an extremely important application value in the field of control of the aphids and can be applied to the fields of control of the aphids, prediction of aphid situation or early warning of the aphids.

The invention discloses a low-density breeding method capable of maintaining a wingless state of acyrthosiphon pisum viviparous female. The low-density breeding method includes the steps of planting of broad beans, cultivation of aphids, group experiments, observation and data recording. The low-density breeding method has the advantages of simple operation, stable state, no seasoning restrictionand the like, materials for studying a wing-shaped differentiation mechanism of aphids are provided, and a good foundation is laid for studying the aphid pest control.

The invention provides a biological assaying method for evaluating the effect of transgene cotton on chrysopa pallens. The method comprises the following steps: paving agar on the bottom of a culture dish; placing transgene cotton leaves on the surface of the agar, inoculating newly-hatched helicoverpa armigera larvae; feeding newly-hatched chrysopa pallens larvae for 1 to 3 days with pea aphid, then feeding the chrysopa pallens larvae with the helicoverpa armigera larvae which has been fed by the transgene cotton leaves for 1 to 3 days; observing the growth situation of the chrysopa pallens larvae since the first day when the chrysopa pallens larvae is fed with the helicoverpa armigera larvae until the chrysopa pallens larvae pupate and perform eclosion; setting a reference group; comparing the differences of the experiment group and the reference group on the indexes of pupation rate, eclosion rate, larva weight, imago weight, and total growth time, and analyzing the difference significance through variance analysis so as to evaluate the effect of transgene cotton on chrysopa pallens. The application range of the biological assaying method is wide. The effect of high dose protein in transgene plants on predators can be effectively measured by utilizing the nutrition relationship evaluation of transgene plant-pest-predator.

The invention relates to an artificial mass propagation method of harmonia axyridis. Two facilities of a seedling raising greenhouse and an insectary are adopted, broad beans are used as host plants for propagating pea aphids which are used as aphids for feeding the harmonia axyridis, and the harmonia axyridis which is used for agricultural control over the aphids can be manually propagated in a large quantity through specific technical measures of broad bean plant breeding, pea aphid inoculation and propagation, artificial breeding of the harmonia axyridis and the like. Healthy broad bean sprouts are bred with broad bean seeds, the pea aphids are inoculated with the broad bean sprouts and propagated, then the pea aphids are collected to feed larvas and adults of the harmonia axyridis, and a large quantity of the larvas and the adults of the harmonia axyridis are propagated to be released in agricultural production areas to control the aphids of all kinds, so that the biological control over the aphids is realized, and the pollution and safety problems caused by chemical control is avoided or reduced.

The invention provides application of 4-hydroxylonchocarpin in preparation of a medicine for preventing and treating aphids, and belongs to the technical field of pesticides. The invention provides an application of 4-hydroxylonchocarpin in the preparation of a medicine for preventing and treating aphids, and relates to the field of medicine for preventing and treating aphids. The 2, 4-hydroxyloncharpin has ideal contact activity on various aphids such as aphis sativa, sitobion avenae and pea aphid, is wide in aphid killing spectrum, is separated from Chinese herbal medicine eleocharis tuberosa peel, has the advantages of being environmentally friendly, high in selectivity, not prone to generating drug resistance of pests and the like, and can be developed as a plant source aphid killing lead compound.

The invention discloses a method for workshop large-scale production of pea aphids. The method includes: S1, seed treatment; S2, matrix soil filling and sowing; S3, seedling pot watering; S4, seedlingemergence and inoculation; S5, aphid re-inoculation; S6, feed cutting; S7, aftertreatment, to be specific, soaking broad bean seeds in water for 24h, taking out, airing for 12h to keep for seeding, filling seedling pots in length of 33.5cm, width of 27cm and height of 12cm with 3-5cm of Funong matrix soil, picking plump broad bean seeds, planting in a proportion of 10*10 (line*row), covering withsoil of 10cm after planting, watering the seedling pots by a watering can, watering 500ml of water for the first time to make bottoms of the seedling pots wet, watering 200ml of water one day beforeinoculation (when pea seedlings are just germinated), watering once every two days after seeding inoculation, watering 200ml of water in each time, performing inoculation of the pea aphids when the pea seedlings grow to 2-4cm, gently cutting a strain from breeding pea aphids, quickly shaking into a small container, picking out 30-40 breeding pea aphids with a line drawing pen, and putting on the pea seedlings.