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A method and feeding device for evaluating the effect of insecticidal protein on adult beetles

A technology of pleura and insecticidal protein, which is applied in the field of evaluation methods and feeding devices for the impact of exogenous insecticidal proteins on the natural enemy insects of pleura, which can solve the risk of affecting pleura, which has not been considered, Transgenic plant pollen can not be achieved and other problems, to achieve the effect of simple structure, improved survival rate, and increased egg production

Inactive Publication Date: 2020-11-13
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] When evaluating the impact of exogenous insecticidal proteins on the mole beetle, the exogenous insecticidal protein that is more than 10 times the maximum exposure to the mole beetle is generally used to feed the mole beetle, and the direct use of transgenic plant pollen cannot achieve the above-mentioned Require
In previous evaluations of the effects of exogenous insecticidal proteins on Coccinella mori, the risk of the interaction between secondary metabolites in plants and insecticidal proteins to affect Copa aureus was not considered

Method used

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  • A method and feeding device for evaluating the effect of insecticidal protein on adult beetles
  • A method and feeding device for evaluating the effect of insecticidal protein on adult beetles
  • A method and feeding device for evaluating the effect of insecticidal protein on adult beetles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Fructose improves the survival rate and egg production of adults of Coccinella gracilis

[0032] Collect 100g of healthy non-transgenic cotton leaves, add 200g of sterile distilled water after grinding, put it in a container, leaching at room temperature in a 200rpm shaker for 2h, centrifuge at 12,000g for 10min with a centrifuge, extract the supernatant, and filter it. Cotton juice extract.

[0033] Weigh 0.4g of fructose and 0.4g of sucrose into 2.0ml centrifuge tubes respectively, then add cotton juice extract, dilute to 1mL, and mix well to obtain 0.4g / ml fructose solution and 0.4g / ml fructose solution. ml of sucrose solution.

[0034] Dispense 0.4g / ml fructose solution and 0.4g / ml sucrose solution into 1.5mL centrifuge tubes, 250μL per tube, cover the tube cap, pierce the centrifuge tube cap with a sharp object, and insert a capillary tube of appropriate length. (inner diameter 0.53mm) to the bottom of the solution, drain the solution in it, cut off th...

Embodiment 2

[0040] Example 2: Evaluation of the influence of exogenous insecticidal protein Cry1Ac protein on Coccinella gracilis

[0041] Collect 100g of healthy non-transgenic cotton leaves, add 200g of sterile distilled water after grinding, put it in a container, leaching at room temperature in a 200rpm shaker for 2h, centrifuge at 12,000g for 10min with a centrifuge, extract the supernatant, and filter it. Cotton juice extract.

[0042] Weigh 20μg of exogenous insecticidal protein Cry1Ac and 400mg of fructose into a 2.0ml centrifuge tube, then add cotton juice extract to dilute to 1mL, and mix well to obtain the prepared exogenous insecticide with a concentration of 20μg / ml. worm protein Cry1Ac solution containing 0.4g / ml fructose solution.

[0043] Weigh 400 μg of protease inhibitor E-64 and 400 mg of fructose into a 2.0 ml centrifuge tube, add cotton juice extract to dilute to 1 mL, and mix well to obtain a prepared solution containing 400 μg / ml of protease inhibitor. Positive co...

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Abstract

The invention discloses a method for evaluating the influence of insecticidal protein on adults of propylaea japonica and a feeding device. The invention firstly designs a feeding device for simulating the plant gland, and the suction mode of cotton flower extrafloral nectaries is simulated, and then a cotton juice extract is used as a solvent to dissolve the exogenous insecticidal protein, and fructose is added to serve as a phagostimulant of the propylaea japonica adults; a single-pair of the propylaea japonica adults are put into the feeding device with a feeding solution, and enough pea aphids are supplemented to culture in an illumination cultivation box. According to the indexes of the to-be-detected solution on the death rate of the propylaea japonica adults, the egg laying early stage, the egg laying amount and the weight, whether the insecticidal protein has the influence on the propylaea japonica adults or not is judged. On the base of establishing the risk theory of evaluating that the secondary metabolites and the insecticidal protein in the plant interact to influence the propylaea javanica, the natural environment of the insecticidal protein threat which is faced by the propylaea ladyridis can be reflected more truly, the evaluation method is simple, and the applicability is high.

Description

technical field [0001] The invention relates to the field of environmental safety of transgenic plants, in particular to a method and a feeding device for evaluating the influence of an exogenous insecticidal protein on adults of the natural enemy insect, Lady Beetle. Background technique [0002] Bacillus thuringiensis is a Gram-positive bacterial genus and is one of the most widely used microbial insecticides in the world. The main source of its insecticidal activity is the parasporal crystal protein produced by the strain. Researchers have introduced Bt genes into conventional crops to obtain transgenic crops expressing exogenous insecticidal proteins. Since the first planting of transgenic crops in 1996, transgenic crops have been widely promoted around the world, creating huge economic benefits for agricultural production around the world. , social and environmental benefits. [0003] Transgenic crops with exogenous insecticidal protein genes can express exogenous inse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033G01N33/50
CPCA01K67/033G01N33/5085
Inventor 张帅崔金杰雒珺瑜王丽
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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