A kind of method for purifying and propagating ladybug
A kind of technology of mole-shaped ladybug and larva, which is applied in the field of artificial purification and multiplication of mole-shaped ladybug, can solve the problems of manual purification trouble, unfavorable egg retrieval and egg collection, etc. easy effect
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Embodiment 1
[0025] The indoor artificial rearing method (purification+propagation) of embodiment 1 moire ladybug
[0026] 1. Catch ladybugs from the field and put them in insect cages (called No. 1 insect cage, and the ladybugs in this insect cage are called the first generation of ladybugs). There are broad bean seedlings (planted in small flowerpots) connected with enough pea aphids. The height of the broad bean seedlings should be 9-17cm as much as possible, and the number of stretched leaves should be as few as possible. Wrap a circle of wet plastic wrap around the small planter. Change the plastic wrap at 9:00, 15:00, and 21:00. Cut the egg mass from the replaced plastic wrap and put it in a fresh-keeping box covered with wet gauze. The feeding conditions of the tortoise beetle are temperature 25±1°C, humidity 70±10%, and photoperiod 16L:8D.
[0027] 2. The hatched larvae are picked out and placed in a new insect cage, which also has broad bean seedlings connected with pea aphids ...
Embodiment 2
[0031] Embodiment 2 increases the test of humidity hatching ovum
[0032] 1. At 24:00, preservative film is wrapped on the small flowerpot of broad bean sprouts in the No. 5 insect cage of embodiment 1, and the eggs are collected, and the preservative film is collected at 7:50 the next morning, and the egg masses are cut off. Place them in culture dishes with two kinds of humidity respectively. One culture dish is covered with wet gauze at the bottom (placed vertically, no water drops will drip down for 20 seconds), and the other culture dish has no wet gauze. A total of 6 days of eggs were collected, 6 groups of experiments (January 8th to January 13th, 2017).
[0033] 2. Observe the start time of hatching of 6 groups of test eggs, and take the time when more than half of the larvae have just hatched as the hatching time.
[0034] Table 1 Comparison of hatching time under different humidity
[0035] humidity Experiment 1 Experiment 2 Experiment 3 Experiment...
Embodiment 3
[0038] The test of embodiment 3 preservative film collecting ovum method
[0039] Carry out the collection of ovum with preservative film, filter paper, broad bean blade in No. 5 insect cages of embodiment 1, and filter paper is circular diameter 15cm, close to be placed on the side of planting broad bean seedling container in the insect cage, about 50 in the cage First adult (31 females). Subject to three days. Check the amount of eggs collected each day. The time is from December 7th to December 9th, 2016.
[0040] Table 2 Egg quantity (grain) of different devices
[0041] device December 7th December 8 December 9th plastic wrap 275 217 258 filter paper 0 0 7 broad bean leaves 183 121 153
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