Composite material for enriching viruses in droplets and application of composite material

Abstract

The invention belongs to the technical field of composite materials, and discloses a composite material for enriching viruses in droplets and application thereof, the composite material for enriching viruses in droplets comprises an ionic liquid component and a polymer component; the ionic liquid component is amino acid ionic liquid, and the amino acid ionic liquid is anionic amino acid ionic liquid. The composite material for enriching the viruses in the droplets can efficiently capture and enrich virus aerosol in the droplets, fully releases the captured and enriched viruses into a nucleic acid extraction buffer solution, keeps a virus sample not damaged after capture, and does not interfere with the subsequent process of nucleic acid detection. According to the invention, the problem of charge loss of commercial electret polymer fibers is solved, and capture and enrichment of virus aerosol in the presence of water are completed by applying a tiny voltage. Compared with a preparation method in the prior art, the material disclosed by the invention is simple in preparation mode, can be prepared by a soaking method, and is easy for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of composite materials, and in particular relates to a composite material for virus enrichment in droplets and an application thereof. Background technique [0002] In the face of the epidemic, it is essential and crucial to detect and prevent people who may be carrying or infected with the new coronavirus. Nucleic acid detection is one of the most universal and effective methods among current virus detection methods. Under normal conditions, nucleic acid testing is divided into: 1. Sample collection. Routine sample types include throat swabs, nasal swabs, sputum, and feces. 2. Extraction of viral nucleic acid. The extraction of nucleic acid is completed by a nucleic acid extraction kit. 3. Reverse transcription and PCR amplification of the extracted viral nucleic acid, and detection by fluorescence analysis. [0003] At present, the process of sample collection in nucleic acid testing is mainly invasive ...

AI Technical Summary

Problems solved by technology

Differences in the sampling methods of different people lead to false negative results in this single, random sampling, which may cause missed diagnosis of infected people and cause secondary infections (false negative results can be as high as 30%, refer to CN 112168220 B)

2. Abnormal reaction of invasive sampling

3. High infection risk for medical staff

While collecting nucleic acid samples, medical staff are bound to have close contact with patients, which greatly increases the risk of infection

4. High labor cost and huge sampling pressure

[0013] The shortcomings of the first type of technology in technology 2 are consistent with the shortcomings of the second type of technology in technology 1, which is that the binding of proteins or the specific binding of antibody receptors is strong, making it difficult for the captured virus to be extracted in the subsequent nucleic acid extraction solution. Sufficient release is not conducive to the subsequent reverse transcription process and amplification process, and does not meet the second requirement of the material

[0015] (1) The existing invasive sampling technology has problems such as uncertainty of results, abnormal reaction of invasive sampling, high infection risk of medical staff, high labor cost and huge sampling pressure

[0016] (2) The existing virus enrichment material, because its own charge is the charge added by electrostatic electret, so in the presence of droplets, the contact of water vapor will cause the rapid loss of electret charge, and it does not have good virus enrichment ability The combination of protein or the specific binding effect of antibody receptor is strong, which makes it difficult for the captured virus to be fully released in the subsequent nucleic acid extraction solution, which is not conducive to the subsequent reverse transcription process and amplification process, and does not have a good sample release ability

[0017] (3) Existing virus enrichment materials, conventional charged or magnetic nanoparticles are usually transition metal elements, which are highly destructive to viruses and will damage the collected virus samples, which cannot meet the requirements of materials for samples. non-destructive requirements

Therefore, the obtained virus-enriched material needs to meet the above-mentioned various properties, and the design and realization of the material are difficult

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