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Gosling plague virus originated from muscovy ducks

A technology of gosling plague virus and Muscovy duck, which is applied in the field of Muscovy duck-origin gosling plague virus, can solve the problems such as the reduction of vaccine potency, and achieve good safety and the effect of preventing the infection of gosling plague virus in young Muscovy ducks

Active Publication Date: 2016-08-31
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, due to the reduction of the potency of the vaccine used due to virus mutation, it is also necessary to screen out the mutated virus strain to prepare a vaccine with higher efficacy

Method used

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  • Gosling plague virus originated from muscovy ducks
  • Gosling plague virus originated from muscovy ducks
  • Gosling plague virus originated from muscovy ducks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1, the screening of YBGPV-M strain

[0011] In 2013, a disease characterized by diarrhea and embolism due to the shedding of some duck intestinal mucosa appeared in a group of young muscovy ducks in a duck farm in Fujian Province. The incidence rate was 50% to 80%, and the fatality rate was 45% to 70%. The use of antibiotics, Muscovy duck parvovirus vaccine and Muscovy duck parvovirus hyperimmune yolk antibody could not control the disease. The inventor aseptically collected the liver, spleen and pancreas of dying ducks, homogenized the liver, spleen and pancreas with sterile normal saline to make a 20% suspension, centrifuged at 3000r / min for 15min, took the bacteria from the upper part and passed through the allantoic cavity Inoculate 11-day-old muscovy duck embryos, hatch for 168 hours, collect the allantoic fluid and embryo body tissue of the dead embryos after 24 hours, homogenize, freeze and thaw three times, and take the supernatant for freezing. Afte...

Embodiment 2

[0014] Embodiment 2 prepares vaccine

[0015] 1. Preparation of virus liquid for seedling production Dilute the YBGPV-M strain 100 times with sterile saline, inoculate 11-12 day-old susceptible Muscovy duck embryos in the allantoic cavity, 0.2ml per embryo, and incubate at 37°C , according to embryo inspection 2 times a day. Select duck embryos that died within 48 to 168 hours after inoculation, place them at 2 to 8°C for 4 to 12 hours, open the air chamber under sterile conditions, and pick out embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantois For embryos with mild edema, take the embryo body (remove the head and limbs) and put it into a tissue grinder to crush, collect allantoic fluid and amniotic fluid, homogenate with embryo fluid, take the crushed tissue and add it at a ratio of 1:3 Physiological saline was mixed, freeze-thawed 3 times, centrifuged at 4000r / min for 30min, the supernatant was taken and mixed ...

Embodiment 3

[0039] 1. Preparation of antigens for seedling production

[0040] 1. Preparation of virus liquid for seedling production Dilute the YBGPV-M strain 100 times with sterile saline, inoculate 11-12 day-old susceptible Muscovy duck embryos in the allantoic cavity, 0.2ml per embryo, and incubate at 37°C , according to embryo inspection 2 times a day. Select duck embryos that died within 48 to 168 hours after inoculation, place them at 2 to 8°C for 4 to 12 hours, open the air chamber under sterile conditions, and pick out embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantois For embryos with mild edema, take the embryo body (remove the head and limbs) and put it into a tissue grinder to crush, collect allantoic fluid and amniotic fluid, homogenate with embryo fluid, take the crushed tissue and add it at a ratio of 1:3 Physiological saline was mixed, freeze-thawed 3 times, centrifuged at 4000r / min for 30min, the supernatant...

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Abstract

The invention aims at providing a gosling plague virus originated from muscovy ducks. The gosling plague virus YBGPV-M strain originated from muscovy ducks is preserved in China Center for Type Culture Collection (CCTCC) on March 31st, 2016 with the preservation serial number of CCTCC NO: V201620. The screened gosling plague virus originated from muscovy ducks is used preparing a vaccine. The gosling plague virus YBGPV-M strain which is originated from muscovy duck and is high in virus content and good in immunogenicity is selected and then is inoculated to duck embryos, infected embryos and allantois liquid are collected and subjected to homogenation, ultrafiltration and concentration and inactivation with a formaldehyde solution, an oil emulsion is added and mixed for emulsion, and the vaccine is prepared. The prepared vaccine has the advantages of being efficient and high in safety.

Description

technical field [0001] The invention belongs to the technical field of pathogenic microorganism screening, and in particular relates to a muscovy duck-derived gosling plague virus. Background technique [0002] Goose parvovirus disease, also known as Derzsy's disease, commonly known as goose flu, goose or gosling plague, foie hepatitis, goose enteritis, etc., is a highly contagious disease that affects young geese and Muscovy ducks. The diversity of disease names reflects multiple pathological features of the disease. According to the age of infected goslings and ducklings, the disease can be manifested as acute, subacute and chronic. The acute type can cause 100% death of goslings within 10 days of age. Many countries have also reported a completely different antigenic Muscovy duck parvovirus infection with a mortality rate as high as 80%. The disease mainly occurs in 1 to 3-week-old gooses and young Muscovy ducks, especially those about 1 week old are more susceptible, ...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/12A61P31/20C12R1/93
CPCA61K39/12A61K2039/5252C12N7/00C12N2750/14321C12N2750/14334Y02A50/30
Inventor 邹敏吴发兴张志鸿朱艳梅孙健刘蕾
Owner YEBIO BIOENG OF QINGDAO
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