61results about How to "The results show that the image is accurate" patented technology

A test paper used for quickly detecting residue of tylosin consists of support layer, absorption layer and protection membrane. It is featured as forming said absorption layer by setting fiber layer, absorption gold mark antibody fiber layer, cellulose membrane layer and water absorption layer in sequence from test end to handle end; setting linear detection signet and linear comparison signet on said cellulose membrane layer and arranging said detection signet and said comparison signet to be in parallel mode.

A colloidal gold chromatographic test strip for rapidly testing lead ion belongs to the field of immunological technique. The test strip provided by the invention is composed of a liner plate and a sample pad, a colloidal-gold combination pad, a coating membrane and a water absorption pad which are successively connected on the liner plate. The colloidal-gold combination pad is glass fiber cotton for adsorbing lead ion colloidal-gold antibody. A linear invisible detection line T printed by a lead ion and carrier protein coupled solution and a linear contrast line C printed by a goat-anti-mouse IgG solution are successively arranged on the coating membrane, wherein the two lines are parallelly arranged. The colloidal gold chromatographic test strip for rapidly testing lead ion in the invention has strong specificity and high sensitivity, and can be used to more rapidly, sensitively and simply test lead ion residues. By the adoption of the test strip, the determination result is vivid, visual, accurate, simple and clear, and false positive and false negative human misjudgments are not easy to occur. The test strip provided by the invention can be used to save cost, has a wide application range, is convenient for popularization, and has an extensive market prospect as well as obvious economic and social benefits.

The invention discloses an enzyme linked immunosorbent assay kit used for detecting the content of heavy metal copper ions in a sample. The kit includes a coat antigen coated enzyme label plate, an enzyme marker, a copper ion chelate specific monoclonal antibody or polyclonal antibody, a copper ion chelate standard solution, a substrate color development solution, a terminating solution, a lotion, and a diluted chelating agent EDTA. The enzyme linked immunosorbent assay kit used for copper ion detection has the advantages of: (1) strong specificity, high sensitivity, a lowest detection limit of 0.42 microgram / L, and a detection range of 2.83-456.04 microgram / L; (2) simplicity, rapidity, and strong timeliness; (3) vivid, visual and accurate result display; and (4) low cost, wide application range, convenient popularization, on-site supervision and suitability for screening a large number of samples.

The invention relates to a test paper strip for rapidly detecting traces of chlorothalonil and a preparation method thereof. The test paper strip is characterized in that a supporting layer is used as the bottom layer, an adsorption layer is used as the intermediate layer, a protective layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fibrous layer, a gold-labeled antibody fibrous layer, a cellulose film and an absorbent material layer at the handle end in order from a test terminal, wherein the cellulose film is provided with detection blots printed by using a chlorothalonil coupling carrier protein solution and control blots printed by using a goat anti-mouse IgG or rabbit anti-mouse IgG (or goat anti-rabbit IgG) antibody solution; the gold-labeled antibody is a colloidal gold labeled chlorothalonil monoclonal antibody or polyclonal antibody; and the chlorothalonil coupling carrier protein is bovine serum albumin, chicken ovalbumin or haemocyanin. The test paper strip disclosed herein has the advantages of strong specificity, high sensitivity, simple, and accurate detection, low cost, wide application scope, and easiness in popularization and application.

The invention relates to a preparation method of a colloid gold chromatography test strip for rapidly detecting furacilin metabolites, belonging to the detection technology field. The strip comprises a liner and a sample pad, a gold labeled binding pad, a packed capsule and a water absorbing pad which are connected successively on the liner. The gold labeled binding pad is made from glass fiber cotton for absorbing SEM derivative gold labeled antibodies of the furacilin metabolites. A detecting line (T) which is printed by the solution of SEM derivative coupling vector protein and a control line (C) which is printed by goat anti-rabbit IgG solution are arranged on the packed capsule. The two lines are parallel arranged. The colloid gold chromatography test strip is prepared based on polyclonal antibodies with high affinity which are labeled by colloid gold. The test strip has the advantages of strong specificity, high sensitivity, convenience, rapid speed, strong timeliness and onsiteoperation. The lowest detected amount can reach to 5ppb. After the test strip is added in a sample solution to be detected, the detecting result can be judged within 15 minutes. The preparation method of the colloid gold chromatography test strip for rapidly detecting the furacilin metabolites has the advantages of visual, directviewing and accurate result display, cost saving performance, wide application range and convenient popularization.

The invention relates to a colloid gold chromatographic test paper strip and a preparation method thereof for fast detecting sibutramine, belonging to the field of detection technique of biological immunoassay methods. The test paper strip comprises a scaleboard, a sample pad, a gold-marking binding pad, a coated film and a water absorbent pad, wherein the sample pad, the gold-marking binding pad, the coated film and the water absorbent pad are sequentially spliced on the scaleboard; the gold-marking binding pad is glass cellucotton for absorbing gold-marking antibodies of sibutramine ramifications; the coated film has a linear adelomorphic detection line T printed by carrier protein solution coupled by the sibutramine ramifications and a linear adelomorphic contrasting line C printed by goat anti-rabbit IgG solution, and the two lines are arranged in parallel. The test paper strip has strong specificity, high sensibility, strong timeliness detected minimum reaching 5ppb, visual, directly-viewing and accurate result display and wide scope of population, is simple, convenient and fast, does not need other reagents and instruments, can be operated in field, can determine detection results within 15 minutes when being added to detected sample solution, and saves cost.

The invention discloses a clopidol colloidal gold chromatography detection test strip or card which comprises a lining board, a sample pad, a gold mark combined pad, a nitrocellulose membrane and a glass water-absorbing pad, wherein the sample pad, the gold mark combined pad, the nitrocellulose membrane and the glass water-absorbing pad are sequentially connected on the lining board; the gold mark combined pad is an anti-clopidol monoclonal antibody or a polyclonal antibody glass fiber cotton which is absorbed with a colloidal gold mark; and an invisible detection line printed by using a clopidol-carrier protein conjugate and an invisible comparison line printed by using goat anti-mouse IgG are positioned on the nitrocellulose membrane. The clopidol colloidal gold chromatography detection test strip and card disclosed by the invention has the advantages of high specificity, high sensitivity, easiness, convenience, high speed, high timeliness, vivid, visual and accurate result display, cost saving, wide application range and convenience for popularization.

The invention provides a colloidal gold chromatography test strip for quickly detecting lead ions and belongs to the technical field of immunology. The colloidal gold chromatography test strip consists of a lining plate and a sample pad, a gold labeled combination pad, a coating film and an absorbent pad which are connected with the lining plate sequentially, wherein the gold labeled combination pad is glass fiber cotton for absorbing lead ion gold labeled antibodies; a linear hidden detection T line printed by using solution in which the lead ions are coupled with a carrier protein and a linear hidden contrast line C printed by using goat anti mouse IgG are formed on the coating film sequentially; and the two lines are arranged in parallel. The colloidal gold chromatography test strip for quickly detecting the lead ions has high specificity and high sensitivity, can detect lead ion residues more quickly, sensitively and simply, is vivid, intuitive, accurate, simple and clear in result judgment, avoids manmade misjudgment such as false positive, false negative and the like, saves cost, is convenient to popularize and has wide market prospect, outstanding economic and social benefits and a wide application range.

The invention discloses an immunochromatography test strip for detecting capsaicin in gutter oil and a preparation method thereof. The immunochromatography test strip comprises a supporter and an adsorption layer, wherein the adsorption layer is fixedly arranged on the supporter; an adsorption fiber layer, a gold label antibody fiber layer, a cellulose membrane layer and a water-absorbing materiallayer are sequentially arranged on the adsorption layer from the testing end; the cellulose membrane layer is provided with a hidden detection imprint which is printed by a capsaicin-coupled carrierprotein solution, and a hidden contrast imprint which is printed by goat anti-mouse IgG, rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solutions; the gold label antibody fiber layer is made of glass fiber cotton adsorbed with gold label antibody, and the gold label antibody is nanogold particle-labeled capsaicin monoclonal antibody or multiclonal antibody. The immunochromatography test strip has the advantages that the specificity is strong; the sensitivity is high, and 1.7ng / ml of capsaicin can be detected; the operation is simple and convenient, the detection is quick, the detectionresult can be judged within 5min, the result display is visualized, intuitive and accurate, the application range is wide, and the immunochromatography test strip is suitable for being popularized and applied.

The invention relates to a preparation method of a colloidal gold chromatography test paper tape which can test nitrofurantoin metabolite and belongs to the field of testing technology. The test paper tape consists of a lining board and a sample gasket, a gold standard bonding gasket, peridium membrane and an absorbent gasket which are jointed together in sequence on the lining board. The gold standard bonding gasket is glass fiber cotton which can absorb the gold-standard antibody of AHD derivative of nitrofurantoin metabolite. A test line (T) printed by the coupling carrier protein solution of AHD derivative and a control line (C) printed by Goat anti-Rb IgG solution are arranged in parallel with each other on the peridium membrane. The colloidal gold chromatography test paper tape is prepared on the bases that colloidal gold is used for marking a high-affinity polyclonal antibody. The test paper tape has high specificity and sensitivity, and the minimum limit which can be tested can reach 5ppb. The test paper tape can be used easily and conveniently on the spot without any other test reagent and instruments, and the test result can be obtained quickly, thereby achieving high timeliness. After the test paper tape is put into the tested sample solution, the test results can be determined in 15 minutes, and the results can be shown vividly, directly and correctly. The test paper tape can save cost and has wide scope of application; therefore, the test paper tape can be promoted conveniently.

The invention discloses a quantum dot test strip for rapidly detecting trace fipronil and a preparation method of the quantum dot test strip. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer, a protecting layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fiber layer, a quantum dot labeled antibody fiber layer, a cellulose membrane layer and a water absorbing material layer at a handle end sequentially from the test end, wherein a cellulose membrane is coated with fipronil coupled carrier protein to serveas a detection line, and a quality control line printed by goat anti or rabbit anti-mouse IgG, goat anti rabbit IgG antibody or SPA is also arranged. A test indicates that the test strip has higher specificity and higher sensitivity, limit of visual detection is 1 ng / mL, and the test strip is free of cross reaction with other pesticides or veterinary drugs and has quite great significance in theaspects of guaranteeing food safety and protecting health of consumers.

The invention provides a test strip, and belongs to the field of food safety fast detection. The test strip comprises a baseplate, a sample cushion, a conjugate release cushion, a reaction film and a water-absorbing cushion, wherein the sample cushion, the conjugate release cushion, the reaction film and the water-absorbing cushion are adhered onto the baseplate and in tight contact with one another sequentially; part of the conjugate release cushion is coated with the sample cushion; the reaction film is coated with detection lines made of amantadine-carrier protein conjugate and quality control lines made of goat anti-mouse IgG; the conjugate release cushion is coated with a colloid gold marker made of amantadine. The test strip has the advantages that the observing time of a detection result can be prolonged; the sample absorption cushion can sufficiently absorb a detection solution and be in full contact with a gold-labelled antibody for full reaction, and then chromatography is conducted on the reaction film for reaction, so that error can be reduced effectively. The test strip can be used for further preventing protein in the gold-labelled antibody from losing activity under the action of certain interference ingredients in a detection sample, so that the impact on the combination of the gold-labelled antibody and a coating antigen is avoided.

The invention discloses a colloidal gold chromatography test strip for quickly detecting sibutramine and derivative thereof and a preparation method thereof. The test strip consists of a lining plate and a sample pad, a gold label bonding pad, a coating film and a water absorption pad engaged on the lining plate in turn, wherein on the coating film, a straight detection line T is coated by using carrier protein solution coupled with the sibutramine and the derivative thereof, and a straight contrast line C is coated by using goat-anti-mouse IgG solution. The method comprises the following steps of: preparing artificial conjugated antigens; preparing monoclonal antibodies of the sibutramine and the derivative thereof; preparing colloidal gold solution; and obtaining a colloidal gold marker and the gold label bonding pad for resisting the monoclonal antibodies of the sibutramine and the derivative thereof. The test strip has the advantages of strong specificity, high sensitivity, strong timeliness and vivid and accurate result display, and is simple, convenient and quick.

The present invention discloses a phenobarbital quick detection test paper strip. It contains bottom layer as supporting layer, intermediate layer as adsorption layer and protective layer fixed on the adsorption layer, Said adsorption layer successively includes test end adsorption fibre layer, adsorption gold-labelled amtibody fibre layer, cellulose membrane layer and water-absorbing material layer of handle end. Besides, said invention also provides the concrete structure on the cellulose membrane layer, said structure include linear detection blotting and linear reference blotting.

The invention relates to an anti-isofenphos-methyl monoclonal antibody as well as a preparation method and an application thereof, an amino acid sequence of an antibody heavy chain variable region ofthe anti-isofenphos-methyl monoclonal antibody is as shown in SEQ ID NO: 1, and the amino acid sequence of a light chain variable region of the anti-isofenphos-methyl monoclonal antibody is as shown in SEQ ID NO: 2; compared with the prior art, the monoclonal antibody has the advantages that: 1) the antibody has outstanding performance; 2) a test strip is specific, sensitive, simple, convenient and rapid, and 3) the result is vivid, intuitive and accurate: the detection result of the colloidal gold test strip is judged according to the color development condition on a cellulose membrane, the result display is vivid, visual, accurate, simple and clear; and 4) the cost is saved, the application range is wide, the popularization is convenient, and the monoclonal antibody has wide market prospect and obvious economic and social benefits.

The invention discloses a colloidal gold labeled test strip for rapidly detecting trace fipronil and a preparation method of the colloidal gold labeled test strip. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer, a protecting layer is fixed on the adsorption layer, and the adsorption layer comprises an adsorption fiber layer, a gold labeledantibody fiber layer, a cellulose membrane layer and a water absorbing material layer at a handle end sequentially from the test end, wherein a cellulose membrane is coated with fipronil coupled carrier protein to serve as a detection line, and a quality control line printed by goat anti or rabbit anti-mouse IgG, goat anti rabbit IgG antibody or SPA is also arranged. A test indicates that the teststrip has higher specificity and higher sensitivity, limit of detection is 10 ng / mL, and the test strip is free of cross reaction with other pesticides or veterinary drugs.

The invention relates to a herbicide detection device, and especially relates to a 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip. The 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip comprises a bottom supporting layer, a middle adsorption layer fixed on the supporting layer and an outer protective layer fixed on the adsorption layer, the adsorption layer successively comprises, from a test sample end, a fiber layer, a fiber layer adsorbing gold immnnochromatography carrier protein Xi coupled with 2,4-dichlorphenoxyacetic acid, a cellulose film layer and a water absorption material layer at the handle end, the cellulose film layer is provided with a straight line type detection imprinting printed by a 2, 4-dichlorphenoxyacetic acid carrier protein solution and a straight line type comparison imprinting printed by a sheep or rabbit anti-mouse IgG solution, and the detection imprinting and the comparison imprinting are in parallel and combined arrangement of "I I". The 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip has the advantages of strong specificity, high sensitivity, simple and fast operation, vivid, intuitive and accurate detection result display, low cost, less investment, no need of special equipment and professional staff, and easy popularization and implementation.

The invention relates to a test strip for rapidly detecting trace erythrosine and a preparation method. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer and a protection layer is fixed on the adsorption layer, wherein the adsorption layer is composed of an adsorption fiber layer, a gold-labeled antibody layer, a cellulose membrane layer and a water absorption material layer in sequence from a testing end; the cellulose membrane layer is provided with a detection print printed by an erythrosine coupled carrier protein solution and a contrast print printed by a goat or rabbit anti-mouse IgG (Immunoglobulin G) solution or a goat anti-rabbit IgG solution; an erythrosine gold-labeled antibody is a colloidal gold labeled erythrosine monoclonal antibody or polyclonal antibody; the erythrosine coupled carrier protein solution is a coupled compound solution of erythrosine and a carrier protein. The test strip provided by the invention is strong in specificity and high in sensitivity and is simple, visualized and accurate; an applicable range is wide and the cost is low; therefore, the test strip is easy to popularize and apply.

The invention discloses a test strip for quickly detecting oxolinic acid and a preparation method thereof. A bottom layer of the test strip is a support layer; a middle layer is an adsorbed layer; a protecting layer is fixed on the adsorbed layer; the adsorbed layer is orderly divided into an adsorption fiber layer, a gold-labelled antibody fiber layer, a cellulose membrane layer and an absorbent material layer at the handle end from a test end; the gold-labelled antibody in the gold-labelled antibody fiber layer is an anti-oxolinic acid polyclonal antibody or monoclonal antibody labelled by colloidal gold; a detection imprinting and a contrast imprinting are arranged on the cellulose membrane layer; the detection imprinting is printed by carrier protein coupled to the oxolinic acid; the contrast imprinting is printed by a goat anti-rabbit or rabbit anti-mouse IgG antibody or a goat anti-rabbit IgG antibody. The test strip disclosed by the invention is simple in structure, strong in specificity, high in sensitivity and accuracy, simple and convenient to operate, wide in application range, low in price, and easy to popularize and apply, and has better social and economic value.

The invention relates to a triazophos monoclonal antibody. The triazophos monoclonal antibody is characterized in that the amino acid sequence of a heavy-chain variable region of the antibody is shownas SEQID NO:1, and the amino acid sequence of a light-chain variable region of the antibody is shown as SEQID NO:2. The invention relates to an application of the triazophos monoclonal antibody to colloidal gold detection test paper. The colloidal gold detection test paper comprises a liner, as well as a sample pad, a gold marker combination pad, a cellulose membrane and a water absorption pad which are sequentially arrayed on the liner, wherein the inner part of the gold marker combination pad is adsorbed with a triazophos gold marker antibody, a detection line and a control line are formedin the cellulose membrane, the detection line is printed with a triazophos hapten coupled carrier protein solution, carrier protein adopted in the carrier protein solution is prepared through couplingO-ethyl-O-(1-phenyl-1,2,4-triazole-3-based)-N-(butyric acid based) thiophosphate ester with bovine serum albumin (BSA) in an active ester method, and the control line is printed through a rabbit antimouse IgG antibody. The triazophos monoclonal antibody disclosed by the invention is high in specificity, high in sensibility, simple, convenient and quick to detect, vivid, audio-visual and accuratein result display, wide in application range and convenient to popularize, and fees are saved.

The invention relates to a chlopyrifos monoclonal antibody and a preparation method and application thereof. The chlopyrifos monoclonal antibody and preparation method and application thereof have theadvantages that the specificity and sensitivity are high, the method is simple and rapid, and a result is displayed vividly, visually and accurately; the cost is reduced, the application range is wide, convenience is provided for popularization; and via the prepared chlopyrifos colloidal gold test paper, the result can be determined within minutes according to strips presented after reaction needless of professionals or auxiliary instruments, operation is simple and rapid, the result is visual and accurate, the cost is low, and detection can be carried out in the outside.

Belonging to the field of food safety rapid detection, the invention relates to an olaquindox metabolite residue rapid detection test strip. The test strip comprises a bottom plate, and a sample pad, a conjugate release pad, a reaction film and a water absorption pad that are attached to the bottom plate and are closely connected. Part of the conjugate release pad is covered by the sample absorption pad. The reaction film is coated with a detection line composed of an olaquindox metabolite-carrier protein conjugate and is coated with a quality control line composed of goat-anti-mouse IgG. The conjugate release pad is coated with a colloidal gold marker of the olaquindox metabolite. The test strip can extend the detection result observation time, the sample absorption pad can fully absorb a detection liquid and fully contact a gold-labelled antibody so as to react completely, and then chromatography to the reaction film is carried out to undergo reaction, thus effectively reducing error. The test strip also can prevent some interference components in the detected sample from inactivating the protein in the gold-labelled antibody to affect combination of the gold-labelled antibody and a coating antigen.

The invention discloses immunochromatographic test paper quickly detecting fleroxacin and a preparation method of the immunochromatographic test paper. A bottom layer of the immunochromatographic test paper serves as a support layer; an intermediate layer serves as an adsorption layer; a protective layer is fixed on the adsorption layer; the adsorption layer sequentially comprises an adsorption fiber layer, a gold-labeled antibody fiber layer, a cellulose membrane layer and a water absorption material layer at a handle end from a test end; a detecting blot printed by a fleroxacin coupled carrier protein solution and a control blot goat printed by an anti-mouse IgG antibody solution are arranged on the cellulose membrane layer; and a gold-labeled antibody is a colloidal gold labeled fleroxacin monoclonal antibody. The immunochromatographic test paper is mainly used for quickly detecting fleroxacin in food, is simple in structure, strong in specificity, high in sensitivity and accuracy, easy and simple to operate, wide in application scope, low in price and easy to popularize and apply, and has better social and economic values.

The invention relates to an isocarbophos monoclonal antibody and application thereof to colloidal gold test paper. An amino acid sequence in a heavy-chain variable region of the antibody is shown as SEQ ID NO:1, and an amino acid sequence in a light-chain variable region of the antibody is shown as SEQ ID NO:2. The colloidal gold test paper comprises a substrate, a sample pad, a gold label combination pad, a cellulose membrane and a water absorption pad, and the sample pad, the gold label combination pad, the cellulose membrane and the water absorption pad are arranged on the substrate sequentially. The isocarbophos monoclonal antibody is adsorbed in the gold label combination pad, the cellulose membrane is provided with an invisible detection line and an invisible control line, the invisible detection line and the invisible control line are parallel, the invisible detection line is printed with isocarbophos hapten coupled carrier protein solution, and the invisible control line is printed with a rabbit anti-rat IgG antibody. Results can be judged within several minutes according to visible strips without professionals and any auxiliary instruments, simplicity and convenience in operation, quickness, direct and accurate results and low cost are realized, and testing can be performed outdoors.

The invention discloses a colloidal gold chromatographic test strip for rapidly testing prednisone acetate and a derivative thereof. The colloidal gold chromatographic test strip consists of a lining plate, a sample pad, a gold labeled conjugate pad, an encapsulating film and an absorbent pad which are connected on the lining plate sequentially. The gold labeled conjugate pad is a polyester fiberfelt of a gold-labeled antibody of the prednisone acetate derivative; and the encapsulating film is provided with a straight line type detection line T which is encapsulated by solution of carrier protein coupled with the prednisone acetate derivative and a straight line type reference line C which is encapsulated by solution of goat-anti-mouse IgG, and the two lines are arranged parallelly and vertically. A preparation method comprises the specific steps of: preparing artificial conjugated antigen; preparing a monoclonal antibody of the prednisone acetate derivative; and preparing solution of colloidal gold to obtain a colloidal gold marker for resisting the monoclonal antibody of the prednisone acetate derivative and the gold labeled conjugate pad. The colloidal gold chromatographic test strip for rapidly testing the prednisone acetate and the derivative thereof and the preparation method have the advantages of high specificity, high sensitivity, simpleness, convenience, quickness, high timeliness, and visual and exact result display.

The invention relates to a colloidal gold chromatography test paper strip for quickly detecting perfluoro caprylic acid and a preparation method thereof, and belongs to the technical field of detection of biological immunization methods. The detecting test paper strip consists of a lining board, and a sample pad, a gold labeled combining pad, an enveloping film and a water absorbing pad, which are orderly connected on the lining board, wherein the gold labeled combining pad is glass fiber wool adsorbing gold-labeled antibodies of the perfluoro caprylic acid; and the enveloping film is provided with a linear invisible test line T line which is printed by carrier protein solution coupled by perfluoro caprylic acid, and a linear invisible contrast line C line which is printed by sheep anti-rabbit IgG solution, and the two lines are parallel. The test paper strip has strong specificity, high sensibility with the lowest detection limit of 5ppb, simplicity, convenience, quickness and strong timeliness, does not need any other reagents and instruments, can be operated in field, can determine a detection result in 15 minutes after being put into tested sample solution, has vivid, directviewing and accurate result display, saves cost, has wide applicable range and contributes to promotion.

The invention discloses a famphur colloidal gold rapid test strip. The strip comprises a liner as well as a sample pad, a gold conjugate pad, a cellulose film and a water absorption pad which are sequentially arranged on the liner. A famphur colloidal gold antibody is adsorbed in the gold conjugate pad. The cellulose film is provided with an invisible test line printed with a carrier protein solution coupled to a famphur hapten and an invisible control line printed with a rabbit anti-mouse IgG antibody. The famphur colloidal gold rapid test strip has the advantages of high specificity, high sensitivity, simpleness, rapidness as well as the visualized, intuitive and accurate result showing.

The invention relates to integrated self-amplification indirect competitive immunochromatography test paper. The test paper comprises a supporting layer, an adsorption layer and a protective layer, wherein the adsorption layer and the protective layer are fixed on the supporting layer; the adsorption layer sequentially comprises a sample pad, a conjugate pad I, a conjugate pad II, a cellulose membrane layer and a water absorption material layer which is located at a handle end, wherein the sample pad, the conjugate pad I, the conjugate pad II and the cellulose membrane layer are sequentially distributed from a test end; a detection line and a quality control line are sequentially arranged on the cellulose membrane layer; and the conjugate pad I adsorbs a target monoclonal antibody, and the conjugate pad II adsorbs an artificial antigen marked by a nanometer material and biotin marked by the nanometer material. According to the test paper, the antibodies do not need to be marked, protective liquid is added and dried on the conjugate pad I, folding and damage of the antibodies in the antibody marking process are avoided, and the antibody dosage can be accurately controlled; and the nano-particle labeled artificial antigen is used as a probe, and each antibody intercepted on the detection line can be accurately combined with two labeled antigens, so that signal self-amplification is realized, and the sensitivity of the immunochromatography test paper is higher.