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Colloidal gold chromatographic test strip for rapidly testing prednisone acetate and derivative thereof and preparation method

A technology of prednisone acetate and chromatographic test paper, which is applied in the detection field of biological immunological methods, can solve the problems of complex components of traditional Chinese medicine, unsatisfactory results, and high sampling cost, etc., to achieve image determination, strong timeliness, and not easy to fake The effect of positives and false negatives

Active Publication Date: 2011-01-19
NANTONG EGENS BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in recent years, the means of illegal production by criminals have become increasingly concealed, and because the ingredients of traditional Chinese medicine are relatively complex, the detection is difficult, and sophisticated analytical instruments and professional technicians are required, resulting in a high cost of supervision and random inspection by the regulatory department, but the effect is not satisfactory.

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  • Colloidal gold chromatographic test strip for rapidly testing prednisone acetate and derivative thereof and preparation method
  • Colloidal gold chromatographic test strip for rapidly testing prednisone acetate and derivative thereof and preparation method
  • Colloidal gold chromatographic test strip for rapidly testing prednisone acetate and derivative thereof and preparation method

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Embodiment Construction

[0023] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the embodiments and accompanying drawings. The embodiments are only used to explain the present invention and do not constitute a limitation to the protection scope of the present invention.

[0024] Such as figure 1 , figure 2Shown is a colloidal gold chromatography test strip for rapid detection of prednisone acetate and its derivatives, which consists of a liner 1 and a sample pad 2, a gold standard binding pad 3, a coating film 4 and a liner 1 successively connected on the liner 1 Composed of water-absorbing pads 5, the gold-labeled binding pad 3 is a polyester fiber felt that absorbs gold-labeled antibodies of prednisone acetate derivatives, and the coating film 4 is coated with a carrier protein solution coupled with prednisone acetate derivatives. The detection line T line 6, and the linear control line C line 7 coated with go...

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Abstract

The invention discloses a colloidal gold chromatographic test strip for rapidly testing prednisone acetate and a derivative thereof. The colloidal gold chromatographic test strip consists of a lining plate, a sample pad, a gold labeled conjugate pad, an encapsulating film and an absorbent pad which are connected on the lining plate sequentially. The gold labeled conjugate pad is a polyester fiberfelt of a gold-labeled antibody of the prednisone acetate derivative; and the encapsulating film is provided with a straight line type detection line T which is encapsulated by solution of carrier protein coupled with the prednisone acetate derivative and a straight line type reference line C which is encapsulated by solution of goat-anti-mouse IgG, and the two lines are arranged parallelly and vertically. A preparation method comprises the specific steps of: preparing artificial conjugated antigen; preparing a monoclonal antibody of the prednisone acetate derivative; and preparing solution of colloidal gold to obtain a colloidal gold marker for resisting the monoclonal antibody of the prednisone acetate derivative and the gold labeled conjugate pad. The colloidal gold chromatographic test strip for rapidly testing the prednisone acetate and the derivative thereof and the preparation method have the advantages of high specificity, high sensitivity, simpleness, convenience, quickness, high timeliness, and visual and exact result display.

Description

technical field [0001] The invention relates to a colloidal gold chromatography test strip and a preparation method for rapidly detecting prednisone acetate and its derivatives, belonging to the technical field of detection of biological immune methods. Background technique [0002] Prednisone acetate is an adrenal cortex hormone drug with anti-inflammatory, anti-allergic, anti-rheumatic, and immunosuppressive effects. In recent years, some unscrupulous manufacturers have illegally added prednisone acetate and other hormones to traditional Chinese medicines for the treatment of immune diseases (such as lupus erythematosus, arthritis, nephritis, etc.) without approval in order to make huge profits. Patients use these problematic "pure Chinese medicine preparations" without knowing it in the short term, and the curative effect is indeed good, but these effects are actually the effects of hormones. However, long-term use or sudden withdrawal of the drug will cause problems: lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577G01N33/531G01N33/544C07K14/765C07K14/77C07K16/44
Inventor 欧卫军吴迪宏
Owner NANTONG EGENS BIOTECH
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