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Anti-isofenphos-methyl monoclonal antibody as well as preparation method and application thereof

A technology of isofenphos-methyl and monoclonal antibody, which is applied in the direction of chemical instruments and methods, instruments, and analytical materials, can solve the problems of limited application of isofenphos-methyl, unintuitive detection results, and inability to conduct online detection, etc. Achieve the effect of displaying the image of the results, facilitating promotion, and using a wide range

Inactive Publication Date: 2020-02-07
SUZHOU KUAIJIEKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the need for expensive instruments, dedicated operators, and the complexity, high cost, and long time of sample pretreatment, it cannot better meet the requirements of quick and easy on-site detection
[0004] ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA in the rapid detection of isofenphos-methyl is greatly limited.

Method used

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  • Anti-isofenphos-methyl monoclonal antibody as well as preparation method and application thereof
  • Anti-isofenphos-methyl monoclonal antibody as well as preparation method and application thereof
  • Anti-isofenphos-methyl monoclonal antibody as well as preparation method and application thereof

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Experimental program
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Embodiment

[0028] Embodiment: 1, the synthesis of isofenphos-methyl hapten

[0029] Synthesis of the immune hapten O-(methoxy-N-butyrylphosphorothio)isopropyl salicylate. Weigh 2.9g of isopropyl salicylate and dissolve it with acetonitrile; 2 CO 3 8.7g of the mixed solution, after dripping, continue to react for 1 hour, vacuum filter after the reaction, evaporate the solvent to obtain a yellow oil, column chromatography, wash with petroleum ether / dichloromethane, evaporate the solvent to obtain a light yellow oily liquid . Weigh 0.54g of 4-aminobutyric acid and 0.78g of KOH, dissolve in 6mL of methanol, cool in an ice-water bath to 0-10°C, slowly add 1.18g of light yellow oily liquid dissolved in 6mL of methanol solution under stirring, and keep warm for 2 hours , after the reaction was completed, washed with petroleum ether / diethyl ether, adjusted the pH of the water layer to 3.0, extracted with diethyl ether, dried over anhydrous sodium sulfate, and evaporated the solvent to obtain ...

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Abstract

The invention relates to an anti-isofenphos-methyl monoclonal antibody as well as a preparation method and an application thereof, an amino acid sequence of an antibody heavy chain variable region ofthe anti-isofenphos-methyl monoclonal antibody is as shown in SEQ ID NO: 1, and the amino acid sequence of a light chain variable region of the anti-isofenphos-methyl monoclonal antibody is as shown in SEQ ID NO: 2; compared with the prior art, the monoclonal antibody has the advantages that: 1) the antibody has outstanding performance; 2) a test strip is specific, sensitive, simple, convenient and rapid, and 3) the result is vivid, intuitive and accurate: the detection result of the colloidal gold test strip is judged according to the color development condition on a cellulose membrane, the result display is vivid, visual, accurate, simple and clear; and 4) the cost is saved, the application range is wide, the popularization is convenient, and the monoclonal antibody has wide market prospect and obvious economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of pesticide residue immunoassay, in particular to an anti-isofenacyl monoclonal antibody and its preparation method and application. Background technique [0002] Isofenphos-methyl (isofenphos-methyl) is an organophosphorus insecticide with broad-spectrum insecticide and long residual effect period, and is an excellent agent for controlling underground pests. It has strong contact and stomach poisoning effects on pests. The mechanism of action is to inhibit the activity of acetylcholinesterase. Acetylcholine is a neurotransmitter. Excessive acetylcholine overstimulates the acetylcholine receptor, resulting in the death of the target organism. However, residues of isofenphos-methyl can also poison some organisms that were not the intended target. [0003] At present, the detection methods for isofenphos-methyl residues are mainly instrumental analysis methods, including gas chromatography (Gaschromatograph...

Claims

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Application Information

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IPC IPC(8): C07K16/44G01N33/577G01N33/558
CPCC07K16/44C07K2317/33G01N33/558G01N33/577G01N2430/10
Inventor 盛相国
Owner SUZHOU KUAIJIEKANG BIOTECH CO LTD
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