Quantum dot test strip for rapidly detecting trace fipronil and preparation method of quantum dot test strip
A technology of micro-fipronil and quantum dots, which is applied in the field of rapid detection of micro-fipronil quantum dot test strips and its preparation, can solve the problems of poor timeliness, tedious and time-consuming, and inability to carry out on-site real-time monitoring, etc., to achieve a judgment image , saving costs, and protecting the health of consumers
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Embodiment 1
[0036] Such as Figure 1-3 Given, the quantum dot test strip for rapid detection of trace amounts of fipronil in the present invention, the bottom layer is a support layer, the middle layer is an adsorption layer, the protective layer is fixed on the adsorption layer, and the adsorption layer is an adsorption fiber layer, quantum dot Labeled antibody fiber layer, cellulose film layer and water-absorbing material layer at the handle end, in which the carrier protein coupled with fipronil is sprayed on the cellulose film as a detection line, and there are also goat anti- or rabbit anti-mouse IgG, goat anti- Rabbit IgG antibody or SPA printed quality control line.
[0037] The labeled antibody in the quantum dot-labeled antibody fiber layer is a quantum dot-labeled anti-fipronil monoclonal antibody or polyclonal antibody.
[0038] The absorbent fiber layer is made of glass fiber cotton, nylon film, polyvinylidene fluoride film or polyester film; the water-absorbing material laye...
Embodiment 2
[0044] To make a quantum dot test strip for rapid detection of trace amounts of fipronil, firstly prepare a coupling fipronil carrier protein and fipronil quantum dot-labeled antibody, thereby preparing a detection line and a quantum dot-labeled antibody fiber layer; secondly, prepare a goat antibody Or rabbit anti-mouse IgG (or goat anti-rabbit IgG) antibody for the preparation of quality control lines.
[0045] The preparation method of the quantum dot test strip for rapid detection of trace amounts of fipronil of the present invention comprises the following steps:
[0046] 1. Preparation of carrier protein coupled to fipronil (i.e. fipronil carrier protein conjugate)
[0047] (1) The hydrolysis method is used to modify the structure of fipronil. Under the condition of strong alkali, the cyano group (-CN) in fipronil (CNT) is hydrolyzed into carboxyl group (-COOH) to obtain CNT-COOH. Dissolve 20 mg of fipronil standard product in 100 μL of acetone, add the acetone solution...
experiment example
[0063] Experimental example, sensitivity and specificity assay
[0064] Take fipronil solutions with concentrations of 0.2ng / mL, 0.4ng / mL, 0.6ng / mL, 0.8ng / mL, 1.0ng / mL, 1.5ng / mL, 2.0ng / mL and 4.0ng / mL as samples, The detection of fipronil was carried out by using fipronil quantum dot test strips. After 5 minutes, under the irradiation of ultraviolet light, all the test strips showed no color at 1.0ng / mL, 1.5ng / mL, 2.0ng / mL and 4.0ng / mL, and 80% of them showed color at 0.8ng / mL. The detection line of the test strip does not develop color, 30% of the detection lines of the test strip do not develop color at 0.6ng / mL, and the detection lines of the test strips of 0.2ng / mL and 0.4ng / mL all develop color, so it is determined that the sulforaphane The minimum detection concentration of nitrile quantum dot test paper is 1 ng / mL, and the sensitivity is set at 1 ng / mL.
[0065] Chlorothalonil, glyphosate, imophos, chlorpyrifos, parathion, malathion and dichlorvos at a concentration o...
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