Quantum dot test strip for rapidly detecting trace fipronil and preparation method of quantum dot test strip

A technology of micro-fipronil and quantum dots, which is applied in the field of rapid detection of micro-fipronil quantum dot test strips and its preparation, can solve the problems of poor timeliness, tedious and time-consuming, and inability to carry out on-site real-time monitoring, etc., to achieve a judgment image , saving costs, and protecting the health of consumers

Inactive Publication Date: 2018-06-29
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting fipronil residues at home and abroad mainly use physical and chemical analysis methods, mainly chromatography, including liquid chromatography-tandem mass spectrometry (LC-MS / MS), gas chromatography (GC), liquid chromatography-tandem Mass spectrometry (LC-MS / MS), gas chromatography-mass spectrometry (GC-MS), etc., the detection of fipronil by these methods requires a series of complicated pretreatment and purification processes, which are tedious and time-consuming, from sample pretreatment to detection It takes at least 2 days for the results, and requires large-scale specialized equipment and professional technicians to operate, which cannot meet the needs of rapid detection, and the timeliness is poor
The analysis process of the fipronil immunoassay kit is relatively simple, but it also takes 2-3 hours for the detection process, and requires the assistance of a microplate reader, which cannot be monitored in real time on site
At present, the main means of rapid screening of drug residues in test strip detection has unique advantages in the screening of fipronil, and it is a detection technology that needs to be developed first. However, the rapid detection method of fipronil test strips is still blank in China

Method used

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  • Quantum dot test strip for rapidly detecting trace fipronil and preparation method of quantum dot test strip
  • Quantum dot test strip for rapidly detecting trace fipronil and preparation method of quantum dot test strip
  • Quantum dot test strip for rapidly detecting trace fipronil and preparation method of quantum dot test strip

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Experimental program
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Effect test

Embodiment 1

[0036] Such as Figure 1-3 Given, the quantum dot test strip for rapid detection of trace amounts of fipronil in the present invention, the bottom layer is a support layer, the middle layer is an adsorption layer, the protective layer is fixed on the adsorption layer, and the adsorption layer is an adsorption fiber layer, quantum dot Labeled antibody fiber layer, cellulose film layer and water-absorbing material layer at the handle end, in which the carrier protein coupled with fipronil is sprayed on the cellulose film as a detection line, and there are also goat anti- or rabbit anti-mouse IgG, goat anti- Rabbit IgG antibody or SPA printed quality control line.

[0037] The labeled antibody in the quantum dot-labeled antibody fiber layer is a quantum dot-labeled anti-fipronil monoclonal antibody or polyclonal antibody.

[0038] The absorbent fiber layer is made of glass fiber cotton, nylon film, polyvinylidene fluoride film or polyester film; the water-absorbing material laye...

Embodiment 2

[0044] To make a quantum dot test strip for rapid detection of trace amounts of fipronil, firstly prepare a coupling fipronil carrier protein and fipronil quantum dot-labeled antibody, thereby preparing a detection line and a quantum dot-labeled antibody fiber layer; secondly, prepare a goat antibody Or rabbit anti-mouse IgG (or goat anti-rabbit IgG) antibody for the preparation of quality control lines.

[0045] The preparation method of the quantum dot test strip for rapid detection of trace amounts of fipronil of the present invention comprises the following steps:

[0046] 1. Preparation of carrier protein coupled to fipronil (i.e. fipronil carrier protein conjugate)

[0047] (1) The hydrolysis method is used to modify the structure of fipronil. Under the condition of strong alkali, the cyano group (-CN) in fipronil (CNT) is hydrolyzed into carboxyl group (-COOH) to obtain CNT-COOH. Dissolve 20 mg of fipronil standard product in 100 μL of acetone, add the acetone solution...

experiment example

[0063] Experimental example, sensitivity and specificity assay

[0064] Take fipronil solutions with concentrations of 0.2ng / mL, 0.4ng / mL, 0.6ng / mL, 0.8ng / mL, 1.0ng / mL, 1.5ng / mL, 2.0ng / mL and 4.0ng / mL as samples, The detection of fipronil was carried out by using fipronil quantum dot test strips. After 5 minutes, under the irradiation of ultraviolet light, all the test strips showed no color at 1.0ng / mL, 1.5ng / mL, 2.0ng / mL and 4.0ng / mL, and 80% of them showed color at 0.8ng / mL. The detection line of the test strip does not develop color, 30% of the detection lines of the test strip do not develop color at 0.6ng / mL, and the detection lines of the test strips of 0.2ng / mL and 0.4ng / mL all develop color, so it is determined that the sulforaphane The minimum detection concentration of nitrile quantum dot test paper is 1 ng / mL, and the sensitivity is set at 1 ng / mL.

[0065] Chlorothalonil, glyphosate, imophos, chlorpyrifos, parathion, malathion and dichlorvos at a concentration o...

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Abstract

The invention discloses a quantum dot test strip for rapidly detecting trace fipronil and a preparation method of the quantum dot test strip. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer, a protecting layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fiber layer, a quantum dot labeled antibody fiber layer, a cellulose membrane layer and a water absorbing material layer at a handle end sequentially from the test end, wherein a cellulose membrane is coated with fipronil coupled carrier protein to serveas a detection line, and a quality control line printed by goat anti or rabbit anti-mouse IgG, goat anti rabbit IgG antibody or SPA is also arranged. A test indicates that the test strip has higher specificity and higher sensitivity, limit of visual detection is 1 ng/mL, and the test strip is free of cross reaction with other pesticides or veterinary drugs and has quite great significance in theaspects of guaranteeing food safety and protecting health of consumers.

Description

technical field [0001] The invention relates to a device for rapidly detecting trace amounts of pesticides, in particular to a quantum dot test strip for rapidly detecting trace amounts of fipronil and a preparation method thereof. Background technique [0002] Fipronil, trade name Fipronil, English common name Fipronil (fipronil), chemical name (RS)-5-amino-1-(2,6-dichloro-a,a,a-trifluoro-p -Tolyl)-4-trifluoromethylsulfinylpyrazole-3-carbonitrile, the molecular formula is C 8 N 2 Cl 4 , molecular formula C12H4Cl2F6N4OS, molecular weight 437.2, is a phenylpyrazole insecticide developed in 1981 by the French company Rhône-Planck. It has been used since the mid-1990s. It is a broad-spectrum insecticide and veterinary drug widely used in the world. It is mainly used for crop pest control, family pet parasite treatment and control, public health and entertainment venue pest control, etc. Widely used in all aspects. It binds to the γ-aminobutyric acid receptor, effectively i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 张改平魏凤仙胡骁飞邓瑞广滕蔓孙亚宁邢广旭姚静静柴书军邢云瑞
Owner HENAN ACAD OF AGRI SCI
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