Integrated self-amplification indirect competitive immunochromatography test paper and detection method

Abstract

The invention relates to integrated self-amplification indirect competitive immunochromatography test paper. The test paper comprises a supporting layer, an adsorption layer and a protective layer, wherein the adsorption layer and the protective layer are fixed on the supporting layer; the adsorption layer sequentially comprises a sample pad, a conjugate pad I, a conjugate pad II, a cellulose membrane layer and a water absorption material layer which is located at a handle end, wherein the sample pad, the conjugate pad I, the conjugate pad II and the cellulose membrane layer are sequentially distributed from a test end; a detection line and a quality control line are sequentially arranged on the cellulose membrane layer; and the conjugate pad I adsorbs a target monoclonal antibody, and the conjugate pad II adsorbs an artificial antigen marked by a nanometer material and biotin marked by the nanometer material. According to the test paper, the antibodies do not need to be marked, protective liquid is added and dried on the conjugate pad I, folding and damage of the antibodies in the antibody marking process are avoided, and the antibody dosage can be accurately controlled; and the nano-particle labeled artificial antigen is used as a probe, and each antibody intercepted on the detection line can be accurately combined with two labeled antigens, so that signal self-amplification is realized, and the sensitivity of the immunochromatography test paper is higher.

Description

technical field [0001] The invention relates to an immunochromatography detection method, in particular to an integrated "self-amplifying" indirect competitive immunochromatography test paper and a detection method for hapten detection. Background technique [0002] Immunochromatography is a rapid immunoassay technology developed in recent years. Its basic principle is the interaction between antibodies and antigens. It uses nitrocellulose membrane as a carrier to label antigens or antibodies as a tracer. A chromatographic detection technique. Compared with traditional immunoassay methods, immunochromatography has the advantages of strong specificity, accurate detection results, simple equipment operation, rapid measurement, low cost, no need for skilled technicians or expensive equipment, etc., fully meeting the requirements of instant detection . At present, immunochromatography technology has been widely used in medicine, animal husbandry, agriculture and food safety an...

AI Technical Summary

Problems solved by technology

[0003] In the field of food safety, small molecule antigen immunochromatography technology based on the principle of competition is the most widely used. However, the existing detection mode often encounters immune reagents such as antigens and antibodies. ELISA detection methods can be successfully established, but immunochromatographic test strips cannot be successfully established; And in many applications the sensitivity of the test strip still needs to be improved

The main reasons are as follows: 1. The antibody folded during the labeling process, and an excessive amount of antibody was added to stabilize the labeling material; 2. The efficiency of the artificial antigen intercepting antibody was low, and an excessive amount of labeled antibody had to be added in order to improve color development. Improve color development; 3. There is no signal amplification when colloidal gold is used to label antibodies. Fluorescent materials such as quantum dots achieve signal amplification while requiring detection instruments to judge the results, which complicates detection.

The existing small molecule immunochromatography detection mode cannot solve this problem, so a simple and sensitive test strip detection mode is urgently needed to provide a better tool for the development of immunochromatography test strips

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