The invention provides a mildew-proof multi-layer eucalyptus board and a production method thereof. The production method of the multi-layer eucalyptus board comprises the steps of veneer sheet sorting, sheet gluing, cold pressing, hot pressing, standing preserving and ash scraping, sanding, repairing, side cutting and overlaying. The method is characterized in that before first-time hot pressingand last-time hot pressing, the surface periphery, the upper and the lower of a board blank are sprayed with a layer of mildew-proof curing film, glue in the hot pressing process can be protected andprevented from too fast drying, stress in the multi-layer board can be kept, urea formaldehyde glue is in sufficient and complete reaction, the formed multi-layer board is not prone to going mouldy, in the use process by a user, volatile formaldehyde is reduced, after hot pressing, the board is subjected to standing preserving, sanding and overlaying, and through side sawing packaging, the mildew-proof multi-layer eucalyptus board product can be obtained.