The invention belongs to the field of biotechnologies and discloses a detection kit for completely and homogeneously determining
glucagon and a method thereof. The detection kit utilizes a spatial neighborhood
chemiluminescence technology to achieve complete and homogeneous detection of
glucagon and is based on a double-sandwich technique, two
monoclonal antibodies are bound to antigenic determinants of
glucagon molecules respectively, a calibrator or a sample to be tested, a
horse radish peroxidase-labeled anti-human glucagon
antibody, a 9,10-dihydroacridine-labeled anti-human glucagon
antibody are added together into a microplate, the two
monoclonal antibodies and the
antigen in the sample form an
antibody-
antigen-antibody sandwich complex without
coating after incubation, the washing process is omitted, a trigger is added after an
antioxidant is added and evenly mixed, a
luminescence intensity value in a period of time is immediately continuously detected, and the content of the glucagon in the sample to be tested is calculated. The detection kit has the advantages of high stability, rapid and convenient detection, high sensitivity and strong specificity.