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Preparation method and application of macrolide antibiotic sensor based on iron nitride

A technology of macrolides and antibiotics, applied in chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, instruments, etc., can solve the problems of reduced sensitivity of electrochemical sensors, limitations on the application of molecularly imprinted membranes, stability and Reduced reproducibility and other issues, to achieve excellent electron transfer capabilities, lower detection costs, and easy operation

Inactive Publication Date: 2019-01-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process of traditional MIP-ECS, there are disadvantages such as difficult elution of template molecules, difficulty in controlling the thickness of imprinted membranes, and poor reproducibility, which limit the application of molecularly imprinted membranes in electroanalytical chemical sensors.
These problems, especially the difficult control of the thickness of the molecularly imprinted membrane, which leads to the decrease of the sensitivity of the electrochemical sensor, and the technical difficulties that the molecularly imprinted membrane is easily detached from the electrode surface during the elution process, resulting in a decrease in stability and reproducibility, limit the application of MIP_ECS. It is of great research significance and market value to find new synthesis methods for molecularly imprinted polymers, new methods for modifying molecularly imprinted membrane electrodes, and methods for combining molecularly imprinted membranes with substrate materials to solve the problems of preparation and application of MIP-ECS.

Method used

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  • Preparation method and application of macrolide antibiotic sensor based on iron nitride
  • Preparation method and application of macrolide antibiotic sensor based on iron nitride
  • Preparation method and application of macrolide antibiotic sensor based on iron nitride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of FeN-nanoarray

[0031] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0032] (2) Weigh 1 mmol iron nitrate hexahydrate Fe(NO 3 ) 3 ·6H 2 O and 3 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0033] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 100°C for 12 hours to prepare the iron hydroxide nanosheet array precursor body electrode;

[0034] (4) Insert the iron hydroxide nanosheet array precursor electrode obtained in step (3) into ammonia water for 5 seconds and then take it out, heat it to 340°C in ammonia environment and keep it for 8 hours, then continue to naturally Cool down to...

Embodiment 2

[0036] Example 2 Preparation of FeN-nanoarray

[0037] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0038] (2) Weigh 2 mmol iron nitrate hexahydrate Fe(NO 3 ) 3 ·6H 2 O and 6 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0039] (3) Put the disposable disposable electrode processed in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 110°C for 11 hours to prepare the iron hydroxide nanosheet array precursor body electrode;

[0040] (4) Insert the iron hydroxide nanosheet array precursor electrode obtained in step (3) into ammonia water for 15 seconds and then take it out, heat it to 370°C and keep it for 6 hours in an ammonia environment, then continue to naturally Cool d...

Embodiment 3

[0042] Example 3 Preparation of FeN-nanoarray

[0043] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0044] (2) Weigh 3 mmol iron nitrate hexahydrate Fe(NO 3 ) 3 ·6H 2 O and 9 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0045] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 130°C for 9 hours to prepare the iron hydroxide nanosheet array precursor body electrode;

[0046] (4) Insert the iron hydroxide nanosheet array precursor electrode obtained in step (3) into ammonia water for 30 seconds and take it out. In the ammonia environment, heat to 400°C and keep it for 4 hours, then continue to naturally Cool down to ro...

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Abstract

The invention discloses a preparation method of a macrolide antibiotic sensor based on iron nitride, and belongs to the technical field of new nano functional materials and chemical biosensors. The method comprises the following steps: firstly preparing an array of iron nitride nanosheets on a disposable electrode, directly preparing a polydopamine film and a molecularly imprinted polymer using the macrolide antibiotic molecule as a template molecule coated with luminol in situ successively on the array of iron nitride nanosheets one after another by adopting an in-situ growth method and utilizing a large specific surface area and a high adsorption activity for amidogen, and eluting the molecularly imprinted polymer of the template molecule after the template molecule is eluted and the original position of the template molecule became a hole, thereby preparing a macrolide antibiotic sensor based on iron nitride.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemiluminescence sensor. It belongs to the technical field of new nano functional materials and chemical biosensors. Background technique [0002] Macrolide antibiotics (macrolides antibiotics, MA) is a general term for a class of antibacterial drugs with 12-16 carbon lactone rings in their molecular structure, which inhibit bacterial protein synthesis by blocking the activity of peptidyl transferase in 50s ribosomes , a rapid bacteriostatic agent. It is mainly used to treat aerobic Gram-positive and negative cocci, certain anaerobic bacteria, Legionella, Mycoplasma, Chlamydia and other infections. When people eat animal food with residues of such antibiotics, the drug resistance produced by animals will also be transferred to humans, thus endangering human health. At present, the methods for detecting macrolide antibiotic molecules mainly include enzyme-linked immunoassay, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76
CPCG01N21/76G01N27/26G01N27/30G01N27/3278
Inventor 程荣琦张勇杜斌
Owner UNIV OF JINAN
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