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Preparation method and application of a sensor for electrochemical analysis of environmental estrogen

A technology for environmental estrogen and electrochemical analysis, applied in the field of new nano-functional materials and biosensing analysis, can solve the problems of reduced sensitivity, stability and reproducibility of electrochemical sensors, and limited application of molecularly imprinted membranes. Excellent electron transfer ability, convenient operation, and the effect of reducing detection cost

Inactive Publication Date: 2021-03-16
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process of traditional MIP-ECS, there are disadvantages such as difficult elution of template molecules, difficulty in controlling the thickness of imprinted membranes, and poor reproducibility, which limit the application of molecularly imprinted membranes in electrochemical sensors.
These problems, especially the difficult control of the thickness of the molecularly imprinted membrane, which leads to the decrease of the sensitivity of the electrochemical sensor, and the technical difficulties that the molecularly imprinted membrane is easily detached from the electrode surface during the elution process, resulting in a decrease in stability and reproducibility, limit the application of MIP_ECS. It is of great research significance and market value to find new synthesis methods for molecularly imprinted polymers, new methods for modifying molecularly imprinted membrane electrodes, and methods for combining molecularly imprinted membranes with substrate materials to solve the problems of preparation and application of MIP-ECS.

Method used

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  • Preparation method and application of a sensor for electrochemical analysis of environmental estrogen
  • Preparation method and application of a sensor for electrochemical analysis of environmental estrogen
  • Preparation method and application of a sensor for electrochemical analysis of environmental estrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of Co-nanoarray

[0031] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0032] (2) Weigh 1 mmol Co(NO 3 ) 2 and 3 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0033] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 100 °C for 12 hours to prepare the cobalt hydroxide nanosheet array precursor body electrode;

[0034] (4) Insert the cobalt hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, react at 20 °C for 4 hours, take it out and use Immersed twice in deionized water to prep...

Embodiment 2

[0036] Example 2 Preparation of Co-nanoarray

[0037] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0038] (2) Weigh 2 mmol Co(NO 3 ) 2 and 6 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0039] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 110 °C for 11 hours to prepare the cobalt hydroxide nanosheet array precursor body electrode;

[0040] (4) Insert the cobalt hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, react at 30 °C for 5 hours, take it out and use Immersed in deionized water for 3 times t...

Embodiment 3

[0042] Example 3 Preparation of Co-nanoarray

[0043] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0044] (2) Weigh 3 mmol Co(NO 3 ) 2 and 9 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0045] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 130 °C for 9 hours to prepare the cobalt hydroxide nanosheet array precursor body electrode;

[0046] (4) Insert the cobalt hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, react at a temperature of 40 °C for 6 hours, take it out and use Immersed in deionized wat...

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Abstract

The invention discloses a fabrication method of an environmental estrogen electrochemical light-emitting sensor, and belongs to the technical field of novel nanometer functional material and biological sensing analysis. A cobalt hydroxide nanosheet array is fabricated on a disposable throwable electrode, and an electronic medium-containing polydopamine thin film and a molecular imprinting polymertaking environmental estrogen as a template molecule are directly and sequentially fabricated on the cobalt hydroxide nanosheet array by an in-situ growth method according to large specific area, high-activity hydroxyl functional group and amide group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecular imprinting molecule of the template molecule is eluted. Therefore, the environmental estrogen electrochemical light-emitting sensor is fabricated and completed.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemical analysis sensor. It belongs to the field of new nano functional material and biosensing analysis technology. Background technique [0002] Environmental estrogen is a kind of chemical substance that exists in the environment. It has the activity similar to that of estrogen in organisms. After entering the human body, it has the effect of simulating estrogen. It is a class of environmental toxins that seriously endanger human health. Common sources of environmental estrogen include pesticides (such as organochlorine pesticides), second-hand smoke, waste gas (such as toxic gases produced by burning plastic waste), food additives, etc. The detection methods for environmental estrogen mainly include high performance liquid chromatography, gas chromatography, chromatography-mass spectrometry, enzyme-linked immunoassay, radioimmunoassay, etc. However, most of the detection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 刘召壹张勇魏琴
Owner UNIV OF JINAN
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