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Method for ultra-sensitive detection of kanamycin based on DNA/Ni-Fe LDO cubic network structure

A network structure, kanamycin technology, applied in the field of bioengineering, can solve the problems of low sensitivity, difficult to detect low concentration analytes, etc., and achieve the effects of strong catalytic activity, low cost and simple operation

Pending Publication Date: 2021-09-21
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

This method is simple and convenient to operate, but the disadvantage is low sensitivity and it is difficult to detect low concentrations of analytes

Method used

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  • Method for ultra-sensitive detection of kanamycin based on DNA/Ni-Fe LDO cubic network structure
  • Method for ultra-sensitive detection of kanamycin based on DNA/Ni-Fe LDO cubic network structure
  • Method for ultra-sensitive detection of kanamycin based on DNA/Ni-Fe LDO cubic network structure

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0029] A method for ultrasensitive detection of kanamycin based on DNA / Ni-Fe LDO cube network structure described in the present invention, the specific operation steps are as follows:

[0030] (1.1), the preparation of Ni-Fe LDO;

[0031] (1.2), according to the target antibiotic sample, design an aptamer and DNA oligonucleotide chain suitable for the target antibiotic sample, and then modify one end with NH 2 -(CH 2 ) 6 Single-stranded DNA loaded on the surface of Ni-Fe LDO;

[0032] (1.3), carry out pre-annealing treatment to DNA oligonucleotide chain;

[0033] (1.4), adding the pre-annealed DNA oligonucleotide chain to the target antibiotic sample to be detected, mixing, and incubating to obtain a mixed solution containing G-quadruplex;

[0034] (1.5), transfer the obtained mixed solution containing G-quadruplex to Ni-Fe LDO modified wit...

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Abstract

The invention discloses a method for the ultra-sensitive detection of kanamycin based on a DNA / Ni-Fe LDO cubic network structure and belongs to the technical field of biological detection. The method comprises the following operation steps of: kanamycin is detected through the formation of a three-dimensional Ni-Fe LDO / DNA network triggered by aptamer high-specificity recognition; XPS analysis proves that Ni and Fe elements in the Ni-FeLDO are in coordination binding with G-quadruplex on the surface of the Ni-FeLDO, and electrons on the G-quadruplex are transferred into the Ni and Fe elements in the Ni-FeLDO with higher peroxidase-like catalytic activity in the catalytic oxidation process; and therefore, the detected limit of detection (LOD) is reduced to 0.31 fM (S / N = 3). In addition, the content of residual kanamycin in actual milk and urine samples can be accurately detected; and the method has a potential application value in detection of kanamycin residues in an actual sample.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for ultrasensitive detection of kanamycin based on a DNA / Ni-Fe LDO cube network structure. Background technique [0002] In today's society, because of the function of killing pathogenic bacteria, antibiotics have been widely used in agriculture, human medicine, animal husbandry and other fields. However, the discovery that overuse of antibiotics can cause many adverse effects, such as anaphylactic shock, neurotoxicity, and secondary infection by sensitive bacteria, has received much attention from various industries. Therefore, it is very important to quickly and accurately detect the antibiotic content in food and medicine. As an amino acid antibiotic, kanamycin has a strong inhibitory effect on Gram-negative bacteria and is often used as an animal drug in animal husbandry. The accumulation of kanamycin content in the human body is usually achieved...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/78C12Q1/682
CPCG01N33/53G01N21/78C12Q1/682
Inventor 沈薇姚瑶邝敬宇胡涛唐盛毛威潘玉泉
Owner JIANGSU UNIV OF SCI & TECH
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