Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of a tetracyclic antibiotic molecularly imprinted electrochemical sensor

A technology of molecular imprinting and antibiotics, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of reduced sensitivity, stability and reproducibility of electrochemical sensors, and limit the application of molecularly imprinted membranes, and achieve excellent electronic performance. Transfer ability, convenient operation, and the effect of reducing testing costs

Active Publication Date: 2021-02-23
UNIV OF JINAN
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process of traditional MIP-ECS, there are disadvantages such as difficult elution of template molecules, difficulty in controlling the thickness of imprinted membranes, and poor reproducibility, which limit the application of molecularly imprinted membranes in electrochemical sensors.
These problems, especially the difficult control of the thickness of the molecularly imprinted membrane, which leads to the decrease of the sensitivity of the electrochemical sensor, and the technical difficulties that the molecularly imprinted membrane is easily detached from the electrode surface during the elution process, resulting in a decrease in stability and reproducibility, limit the application of MIP_ECS. It is of great research significance and market value to find new synthesis methods for molecularly imprinted polymers, new methods for modifying molecularly imprinted membrane electrodes, and methods for combining molecularly imprinted membranes with substrate materials to solve the problems of preparation and application of MIP-ECS.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of a tetracyclic antibiotic molecularly imprinted electrochemical sensor
  • Preparation method and application of a tetracyclic antibiotic molecularly imprinted electrochemical sensor
  • Preparation method and application of a tetracyclic antibiotic molecularly imprinted electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of NiFeN-nanoarray

[0031] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0032] (2) Weigh 1 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 3 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0033] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reactor in step (2), and react at a temperature of 100 °C for 12 hours to prepare nickel-iron bimetallic layered hydrogen Oxide nanosheet array precursor electrode;

[0034] (4) Insert the nickel-iron bimetal layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, and react at a temperature o...

Embodiment 2

[0036] Example 2 Preparation of NiFeN-nanoarray

[0037] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0038] (2) Weigh 2 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 6 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0039] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 110 °C for 11 hours to prepare nickel-iron bimetallic layered hydrogen Oxide nanosheet array precursor electrode;

[0040] (4) Insert the nickel-iron bimetallic layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, and react at a te...

Embodiment 3

[0042] Example 3 Preparation of NiFeN-nanoarray

[0043] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0044] (2) Weigh 3 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 9 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0045] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 130 ° C for 9 hours to prepare a nickel-iron double metal nitride nanometer Sheet array precursor electrode;

[0046] (4) Insert the nickel-iron bimetal layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine, ammonium persulfate and cobalt nitrate, and react at a temperatur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a fabrication method of a tetracycline antibiotic molecular imprinting electrochemical sensor, and belongs to the technical field of novel nanometer functional material and chemical biological sensor. A nickel-iron thermometal nitride nanosheet array is fabricated on a disposable throwable electrode, an electronic medium-containing polydopamine thin film and a molecular imprinting polymer taking ampicillin as a template molecule polymer are directly and sequentially fabricated on the nickel-iron thermometal layered hydroxide nanosheet array by an in-situ growth method according to large specific surface area, high-activity hydroxyl functional group and amino functional group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecule imprinting polymer of the template molecule is eluted. Therefore, the tetracycline antibiotic molecular imprinting electrochemical sensor is fabricatedand completed.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemical analysis sensor. It belongs to the field of new nano functional material and biosensing analysis technology. Background technique [0002] Tetracyclic antibiotics, also known as ampicillin, is a β-lactam antibiotic, a semi-synthetic broad-spectrum penicillin, which can treat a variety of bacterial infections. Indications include respiratory tract infection, urinary tract infection, meningitis, salmonella infection, and endocarditis. Because of its convenient use and low cost, it is mostly used to treat infectious diseases caused by sensitive bacteria in chickens, such as Escherichia coli, Salmonella, Pasteurella, Staphylococcus and Streptococcus infections. On October 27, 2017, in the list of carcinogens released by the International Agency for Research on Cancer of the World Health Organization, ampicillin was included in the list of category 3 carcinogens. Therefor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 张勇刘召壹杜斌
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com