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Preparation method and application of an environmental estrogen electrochemiluminescence sensor

An environmental estrogen and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions, can solve the problem of reducing the sensitivity of electrochemical sensors, limiting the application of molecularly imprinted membranes, and stabilizing The problems such as low sex and reproducibility are achieved, and the effect of excellent electron transfer ability, low detection cost and convenient operation is achieved.

Inactive Publication Date: 2021-01-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process of traditional MIP-ECS, there are disadvantages such as difficult elution of template molecules, difficulty in controlling the thickness of imprinted membranes, and poor reproducibility, which limit the application of molecularly imprinted membranes in electroanalytical chemical sensors.
These problems, especially the difficult control of the thickness of the molecularly imprinted membrane, which leads to the decrease of the sensitivity of the electrochemical sensor, and the technical difficulties that the molecularly imprinted membrane is easily detached from the electrode surface during the elution process, resulting in a decrease in stability and reproducibility, limit the application of MIP_ECS. It is of great research significance and market value to find new synthesis methods for molecularly imprinted polymers, new methods for modifying molecularly imprinted membrane electrodes, and methods for combining molecularly imprinted membranes with substrate materials to solve the problems of preparation and application of MIP-ECS.

Method used

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  • Preparation method and application of an environmental estrogen electrochemiluminescence sensor
  • Preparation method and application of an environmental estrogen electrochemiluminescence sensor
  • Preparation method and application of an environmental estrogen electrochemiluminescence sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of CoO-nanoarray

[0031] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0032] (2) Weigh 1 mmol Co(NO 3 ) 2 and 3 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0033] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reactor in step (2), react at a temperature of 100 °C for 12 hours, then take it out and dry it at 300 °C The lower annealing treatment was performed for 3 hours, and the cobalt oxide nanosheet array precursor electrode was prepared;

[0034] (4) Insert the cobalt oxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine and ammonium persulfate, react at 20 °C for 4 hours, ...

Embodiment 2

[0036] Example 2 Preparation of CoO-nanoarray

[0037] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0038] (2) Weigh 2 mmol Co(NO 3 ) 2 and 6 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0039] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), react at a temperature of 110°C for 11 hours, then take it out and dry it at 350°C The lower annealing treatment was performed for 2 hours, and the cobalt oxide nanosheet array precursor electrode was prepared;

[0040] (4) Insert the cobalt oxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine and ammonium persulfate, react at 30 °C for 5 h...

Embodiment 3

[0042] Example 3 Preparation of CoO-nanoarray

[0043] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0044] (2) Weigh 3 mmol Co(NO 3 ) 2 and 9 mmol urea CO(NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water and stir until clear, then transfer to a 50 mL polytetrafluoroethylene reactor;

[0045] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reactor in step (2), react at a temperature of 130 ℃ for 9 hours, then take it out and dry it at 400 ℃ The lower annealing treatment was performed for 1 hour, and the cobalt oxide nanosheet array precursor electrode was prepared;

[0046] (4) Insert the cobalt oxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine and ammonium persulfate, react at a temperature of 40 °C ...

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Abstract

The invention discloses a preparation method of an environmental estrogen electrochemical luminescence sensor. It belongs to the field of new nano functional material and biosensing analysis technology. In the present invention, cobalt oxide nanosheet arrays are firstly prepared on disposable disposable electrodes, and polydopamine films and in-situ coatings are successively prepared directly on the iron oxide nanosheet arrays by utilizing its large specific surface area and adopting an in-situ growth method. Molecularly imprinted polymers covered with luminol that use environmental estrogen as template molecules, after the template molecules are eluted, the position of the original template molecules becomes a hole, that is, the molecularly imprinted polymers that elute the template molecules, thus , an environmental estrogen electrochemiluminescence sensor is prepared.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemiluminescence sensor. It belongs to the field of new nano functional material and biosensing analysis technology. Background technique [0002] Environmental estrogen is a kind of chemical substance that exists in the environment. It has the activity similar to that of estrogen in organisms. After entering the human body, it has the effect of simulating estrogen. It is a class of environmental toxins that seriously endanger human health. Common sources of environmental estrogen include pesticides (such as organochlorine pesticides), second-hand smoke, waste gas (such as toxic gases produced by burning plastic waste), food additives, etc. The detection methods for environmental estrogen mainly include high performance liquid chromatography, gas chromatography, chromatography-mass spectrometry, enzyme-linked immunoassay, radioimmunoassay, etc. However, most of the detection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76
CPCG01N21/76G01N27/26G01N27/30G01N27/3278
Inventor 程荣琦张勇魏琴
Owner UNIV OF JINAN
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