A method for measuring photoacoustic signals in biological tissue includes emitting laser pulses into biological tissue to generate photoacoustic field, emitting a focused ultrasonic beam into the photoacoustic field to superpose on photocoustic signal and generate its beat signals, receiving and measuring them, separating signal spectrum, and extracting photoacoustic signals. Its apparatus is composed of laser device, ultrasonic generator, sound signal measurer, signal amplifier, data acquisition card, and computer. It can diagnose the lesion of biological tissue with radiactive damage.