30results about How to "No amplification" patented technology

The invention relates to the field of animal disease molecular biology detection methods and detection agents, in particular to an animal Torque Teno virus rapid detection primer, a kit and a detection method using a loop-mediated isothermal amplification (LAMP) technology. Multiple comparisons are carried out by using ClustalW according to two different serotype gene sequences of an animal Torque Teno virus, and conserved zones of the sequences are analyzed, an inner primer and an outer primer are respectively designed by adopting LAMP primer design software, and detection and identification of TTV1 and TTV2 serotypes are realized rapidly specifically. The animal Torque Teno virus detection method disclosed by the invention has the advantages of rapidness, high sensitivity, strong specificity, convenience for operation, low requirement of equipment, time and labor saving, easiness in observing results, no complex post-treatment, and wide application range.

This application provides a system and method for checking the validity of the installation method of a three-way impact acceleration sensor, including a test ball, an excitation ball, a launcher, a data acquisition system, and a sensor installation effectiveness analysis unit; a three-way impact acceleration sensor is installed on the test ball; The excitation ball is used to impact the excitation test ball; the launch device provides the initial momentum for the excitation ball, so that the excitation ball shoots obliquely to the inspection ball, so as to ensure the three-way and repeatability of the excitation; the data acquisition system is used to receive and store the impact time domain data; the sensor installation effectiveness analysis unit is used to analyze and process the impact data. The beneficial effects of the application are: the launching device obliquely shoots the excitation ball at a controllable speed and collides with the inspection ball, and uses the sensor installation effectiveness analysis unit to process the impact time domain data collected by the sensor to judge the three-way impact acceleration Whether the sensor is effective for spacecraft shock response test in this installation mode.

The invention discloses a constant-temperature fluorescence detection method and a detection kit for shrimp infectious subcutaneous and hematopoietic necrosis virus (IHHNV) RAA. The detection kit includes a forward primer of SEQ ID NO.1, a reverse primer of SEQ ID NO.2, a specific fluorescent probe of SEQ ID NO.3, a reaction solution, a recombinant polymerase and a control substance. The kit of the invention has strong specificity; high detection sensitivity, which can reach 0.32fg / μL; high accuracy and reliability; simple and fast operation, suitable for on-site detection, and has wide application scenarios.

The invention discloses a black fungus strain specific molecular marker primer, a test kit and application thereof. The primer pair is named as 3-ty026-3; the sequence of an upstream primer of the primer pair is 5 '-CGAGAGGCAAGGTGAACTCC-3', and the sequence of a downstream primer of the primer pair is 5 '-GCTGGCAGTCGATCAAGATC-3'. The molecular marker primer pair 3-ty026-3 is high in specificity, genome DNA of the black fungus strain Guiyun No.3 can be specifically amplified, the amplification effect on other black fungus strains is avoided, and the black fungus strain Guiyun No.3 can be rapidly and accurately identified through the specific molecular marker primer pair 3-ty026-3.

The invention discloses a shrimp iridescent virus (SIV) RAA constant temperature fluorescence detection method and a detection kit. The detection kit includes a forward primer of SEQ ID NO.1, a reverse primer of SEQ ID NO.2, a specific fluorescent probe of SEQ ID NO.3, a reaction solution, a recombinant polymerase and a control substance. The kit of the invention has strong specificity; high detection sensitivity, which can reach 2fg / μL; high accuracy and reliability; simple and fast operation, suitable for on-site detection, and has wide application scenarios.

The invention discloses a WSSV prawn white spot syndrome virus (WSSV) LAMP detection primer set and a detection kit. The detection primer set includes a pair of outer primers, a pair of inner primers and a pair of loop primers; the detection kit includes a primer solution, a reaction solution, DNA polymerase and a reference substance; the kit also includes a chromogenic reagent. The kit of the invention is easy and quick to operate, suitable for on-site detection, strong in specificity, high in detection sensitivity up to 1 fg / μL, high in accuracy and reliability, and has wide application prospects.

The invention discloses PCR (polymerase chain reaction) detection primers for a drug resistance gene NDM-1 of bacteria and a detection kit containing the primers. Specific PCR primers of the gene NDM-1 are designed to investigate whether drug resistance bacteria in a farm carries the NDM-1 gene or not, the PCR reaction system and condition are optimized to obtain a PCR detection method with high sensitivity, high specificity and high repeatability for a (the) drug resistance gene NDM-1 of bacteria; and the lowest genome DNA (deoxyribonucleic acid) concentration, which can be detected by the detection method, is 9.18*10<-7>mu g / mu L. The method can be used in early diagnosis and quick screen of drug resistance gene NDM-1 carrying bacteria, epidemiological assessment as means for early diagnosis and quick screen of drug resistance gene NDM-1 carrying bacteria, epidemiological assessment,etc. in livestock and poultry breeding process, and can be applied to monitoring, prevention and control of clinical drug resistance bacteria in current veterinarian.