30results about How to "No need for electrophoretic detection" patented technology

The invention relates to a fluorescent quantitative PCR method for simultaneously detecting type 2 extracellular protein factor EF and hemolysin gene SLY of Streptococcus suis based on a SYBR Green dye melting curve analysis method. According to the conserved sequences of EF and SLY genes of Streptococcus suis type 2 published in GenBank, two pairs of specific primers were designed and synthesized, the PCR reaction system and reaction conditions were optimized, and the simultaneous detection of Streptococcus suis type 2 was established by analyzing the amplification curve and melting curve SYBR GreenI dye fluorescence quantitative PCR method for EF and SLY genes. On this basis, the molar ratio of EF and SLY primers was optimized, and the amplification efficiency of the two pairs of primers was adjusted, so that the peak sizes of the two products in the melting curve were close. The EF and SLY genes of Streptococcus suis type 2 can be detected conveniently and simultaneously by using the established SYBR Green dye melting curve analysis method.

The invention discloses a loop-mediated isothermal amplification primer set for identifying three kinds of pratylenchus pratensis on sugarcane and a kit comprising the loop-mediated isothermal amplification primer set. The loop-mediated isothermal amplification primer set comprises three groups of primers, namely, an outer primer pair PZF3 / PZB3 and an inner primer pair PZFIP / PZBIP, an outer primer pair PPF3 / PPB3 and an inner primer pair PPFIP / PPBIP, as well as an outer primer pair PBF3 / PBB3 and an inner primer pair PBFIP / PBBIP, wherein the nucleotide sequences of the primers are respectively shown as SEQ ID NO.1-12. The three kinds of pratylenchus pratensis are pratylenchus zeae, P. parazeae and P. brachyurus. The loop-mediated isothermal amplification primer set and the kit comprising the loop-mediated isothermal amplification primer set have low requirements on instrument and equipment, are simple, rapid and safe, and are good in specificity and high in sensitivity, and a technical support is provided for the detection for pratylenchus zeae, P. parazeae and P. brachyurus, especially, the rapid identification and distinguishing for the three kinds of pratylenchus pratensis of the grassroots units.