The invention relates to the technical field of probiotic culture, in particular to a culture tank for culturing high-activity probiotics. Heated wet gas is injected into a chamber of the culture tank, so that the temperature and humidity in the chamber of the culture tank meet probiotic culture requirements, the growth environment of probiotics is ensured, the culture quality of the probiotics isimproved, and the use reliability of the device is improved. The culture tank comprises a base, a culture tank body, three sets of supporting legs, a material injection pipe, an upper cover, a discharging pipe, a water storage tank, a water injection pipe, a gas collecting hood, a heating unit, a draught fan support, a draught fan, a gas conveying pipe and a gas return pipe, wherein bottom ends of the three sets of supporting legs are connected with the top end of the base, top ends of the three sets of supporting legs are connected with the bottom end of the culture tank body, the output endof the material injection pipe communicates with a material injection port of the culture tank body, the bottom end of the upper cover is detachably connected with the input end of the material injection pipe through bolts, the input end of the discharging pipe communicates with a discharging port of the culture tank body, a first valve is arranged on the discharging pipe in a communicating mode,and the bottom end of the water storage tank is connected with the top end of the base.