46results about How to "Improve rooting efficiency" patented technology

The invention discloses an ex-vitro rooting method for tissue culture seedlings of pinus massoniana. The ex-vitro rooting method comprises the following steps: taking terminal buds of aseptic seedlings with 0.5cm long hypocotyls as explants for inducing cluster buds in a culture medium I; carrying out elongation culture on the induced cluster buds in a culture medium II, individually cutting off the terminal buds, and inoculating the cut terminal buds into a culture medium III for propagation to obtain subculture buds I; repeatedly elongating the subculture buds I, individually cutting off the subculture buds I, dividing the cut subculture buds I into terminal buds and bud sections, continuously propagating in a subculture propagation culture medium III to obtain subculture buds II, and repeatedly elongating and propagating the subculture buds II to obtain new propagation subculture buds; and transplanting the individually cut 2.5-3.5cm high subculture buds into a seedling culture cup for ex-vitro rooting, and transplanting the ex-vitro subculture buds into a seedling nursery for culture to obtain the ex-vitro tissue culture seedlings. According to the method disclosed by the invention, the production program of test-tube seedlings is simplified, the production cost is reduced, the production efficiency is improved, the rooting treatment time is 25-30 days, the ex-vitro rooting rate reaches 92%-98%, the root system quality is higher, the transplanting survival rate reaches 90%-95%, and the method has better economic, social and ecologic benefits.

The invention relates to a blueberry culture method, in particular to an ex-vitro rooting method for tissue culture blueberry seedlings. The ex-vitro rooting method includes that tissue culture blueberry seedlings which grow strongly are selected and are exercised domesticated; mainly scattered light is emitted in seedling exercising chambers; the seedlings are fetched out from bottles, each seedling is pruned to obtain 2-4 sections, the sections are dipped in rooting liquid before cutting is carried out, moss is sterilized and treated, and is soaked in water one day before being used, and the soaked moss is washed by clear water, is wrung and minced and then is spread on seedling growing discs to obtain cutting media; water is sprayed on the seedlings every now and then in cutting procedures; the seedlings are sufficiently watered after cutting is completed, the environment humidity is controlled and ranges from 80% to 90%, the seedlings are watered every three other days, leaf fertilizers are sprayed on the seedlings once every two other weeks, bactericides are sprayed on the seedlings once every month, and robust blueberry moss seedlings can be acquired after four months, are transplanted into nutrition pots and are planted in the nutrition pots. The ex-vitro rooting method has the advantage that technical problems of large quantities of culture programs, low survival rate, high seedling cost and the like of an existing ex-vitro rooting method for tissue culture blueberry seedlings mainly can be solved.

The invention relates to a slip pepper isolated organism cultivating technique and method, which uses two kinds of organism cultivate techniques to realize the slip pepper regeneration. The invention is characterized in that it overcomes the technique bottleneck of slip pepper isolated organism cultivation as endotrophic mycorrhiza pollution, with which the slip pepper can be cultivated via various methods, to realize the regeneration and quick cultivation of slip pepper and harmless seed via germ cultivation and callus induction. The slip pepper seed cultivated by the invention is germ free and harmless while th cultivate index in each month can reach 7-8, the root rate can reach 100%, and the transplant survival rate can reach 87.2%. The invention has significant social and economic benefits.

Provided is a plant having improved efficiency in propagation by cutting resulting from the enhanced rooting efficiency, and improved vase life. A method of producing a transformed plant having improved rooting efficiency and / or prolonged vase life, comprising transforming a plant using a gene wherein a DNA encoding a protein that binds to a stress-responsive element contained in a stress-responsive promoter and regulates the transcription of a gene located downstream of the element is ligated downstream of the stress-responsive promoter.

The invention discloses a selenium-rich Hylocereus undulates high efficiency planting method. The planting method includes the following steps: (1) pretreatment of Hylocereus undulates branches: cutting stems off from mother plants of Hylocereus undulates, cutting the stems into stem segments with the length of 15-20 cm, soaking the stem segments into a rooting solution, performing auxiliary treatment on the stem segments on a position, 70-100 cm away from an ultraviolet radiator having a distance wavelength of 320-380 nm and the frequency of 2X10<14-16> Hz, for 30-45 min, and acquiring the treated Hylocereus undulates branches; (2) cuttage and seedling cultivation; and (3) fertilizing management: applying a selenium-rich organic fertilizer once every 3 months during growth of the Hylocereus undulates seedlings, and spraying the Hylocereus undulates seedlings with a selenium-rich solution once every 8 days during a period that the Hylocereus undulates branches have buds to a period that flowers fall. The selenium-rich Hylocereus undulates high efficiency planting method can significantly improve the survival rate of Hylocereus undulates branches, can greatly increase the yield and the selenium content of the Hylocereus undulates fruits, and has high market promotion value.

The invention discloses a grafting method of tree-like Chinese roses. The grafting method comprises the first step of loading a matrix into a seedbed, and afterwards laying gauze and black gauze on the seedbed; the second step of selecting branches of Rosa multiflora Thunb. var. carnea Thory as stock cuttings; the third step of inserting the stock cuttings into the matrix, and making the upper ends of the stock cuttings in contact with the gauze; the fourth step of during cultivation, using the black gauze to shade light within 10-14 days after cuttage of the stock cuttings, maintaining the humidity to be 70-75% and the temperature to be 22-25 DEG C, afterwards removing the black gauze, and maintaining the humidity to be 50-55%, wherein when light treatment is conducted, the temperature is 26-30 DEG C, and when dark treatment is conducted, the temperature is 8-10 DEG C; the fifth step of after cultivation is conducted for 4-5 months, the stock cuttings are cultivated into tree-like Chinese rose stocks, in 20 days before transplanting is conducted, using the tree-like Chinese rose stocks as the circle center, digging first annular ditches around the radius of the stocks with the radius being 50-60 cm, in 10 days before transplanting is conducted, digging second annular ditches around the radius of the stocks with the radius being 30-40 cm, and in 2-3 days before transplanting is conducted, digging third annular ditches around the radius of the stocks with the radius being 10-15 cm, and then conducting transplanting when transplanting is needed; the sixth step of conducting tree-like Chinese rose grafting from the middle ten days to the last ten days of October every year.

The invention belongs to an efficient nursery seedling raising technology in the technical field of agriculture and forestry, and particularly discloses a vine tea seedling raising method that can root efficiently in a laminated manner. The vine tea seedling raising method comprises two key technical links: using first-level nursery lamination processing cuttage twigs to promote rooting; using second-level nursery to grow strong seedlings. The method adopts two-segment type seedling raising, and avoids extensive cuttage management; the method adopts a laminated method for seedling raising, uses low temperature in winter to reduce infection and mold rot, greatly promotes cutting slips to form callus, and improves rooting efficiency; with the two-segment type seedling raising and the laminated technology combined, the method improves the survival rate of cuttage and the rate of strong seedlings.

The invention relates to the field of plant growing, and provides a rapid propagation method for calatheaveitchiana. The method is used for improving the propagation efficiency of the calatheaveitchiana. The rapid propagation method for the calatheaveitchiana comprises the steps that a calatheaveitchiana female parent is taken, a lateral bud is taken, part of a leaf sheath is stripped, and then the lateral bud is cleaned, then soaked with ethyl alcohol and immersed into a disinfection solution for disinfection; part of the leaf sheath is stripped, and the lateral bud is immersed into the disinfection solution again for a time and then taken out to be cleaned; the remaining leaf sheath is stripped to obtain an explant; the explant is subjected to adventitious bud induction culture until anadventitious bud seedling is obtained; the adventitious bud seedling is taken, the stem section is obtained and subjected to adventitious bud multiplication culture, rooting induction culture is conducted until a complete plant tissue culture seedling with main roots is obtained; the tissue culture seedling is cleaned and then transplanted into the substrate, and a lot of calatheaveitchiana seedlings are obtained. In the culture process, the seedling survival rate is high, more calatheaveitchiana plants can be obtained within the same culture time, and the propagation efficiency is greatly improved.

A prescription of rooting medium for cultivating cowberry consists of macro element, trace element, iron salt and organic component, etc. of different components and concentration. The rooting medium has high routing efficiency above 95percent. The period for routing cultivation is short and it can be transplanted in less than 20 days requiring no hardening off, and is simple to manage after being transplanted with a survival rate as high as 95percent. Besides, the component of the culture medium is ordinary and easy to confect without special compounds.

The invention discloses a strawberry tissue and seedling culture method. The strawberry tissue and seedling culture method is characterized in that strawberry seeds are soaked in a potassium permanganate solution with the mass concentration of 1-3%, and then sterile water is utilized to flush the soaked strawberry seeds to obtain sterile seeds; the sterile seeds are inoculated into a primary culture medium for primary culture to obtain sterile seedlings; cotyledons and hypocotyls of the sterile seedlings are taken as explants and inoculated into a subculture medium for subculture to obtain rootless seedlings; the rootless seedlings are transplanted into a rooting culture medium for rooting culture to obtain strawberry seedlings. The strawberry tissue and seedling culture method is high in rooting efficiency and survival rate and can maintain the favorable quality of plants.

A cutting seedling rooting agent for zelkova serrata is characterized by being prepared from, by weight, 35-50 parts of ABT rooting powder, 1.5-2.5 parts of portulaca oleracea extracting solution, 2-4 parts of compound sodium nitrophenolate, 2-4 parts of mentha root dry powder, 2-4 parts of purple potato vine dry powder, 0.4-0.6 part of chrysanthemum extract, 1-2 parts of benzoic acid, 0.5-1.5 parts of brassin, 1-2 parts of saponin, 4-6 parts of chelated zinc, 0.4-0.6 part of low-sodium salt, 1.5-2.5 part of humic acid, 0.4-0.6 part of glyceryl behenate, 1.5-2.5 parts of rice vinegar, 1.5-2.5 parts of tea saponin, 1.5-2.5 parts of seaweed micro powder, 0.4-0.6 part of neutral protease, 4-6 parts of 25% carbendazim wettable powder and 0.3-0.5 part of L-glutamic acid. The cutting seedling rooting agent can bring forward rooting and improve the rooting efficiency.

The invention discloses a water culture cuttage rapid propagation method for cinnamomum porrectum, and particularly relates to the technical field of seedling culture. The method comprises the following steps of manufacturing a cuttage water tank, selecting and processing cutting slips, performing cuttage and performing cuttage management. The invention also provides application of the water culture cuttage rapid propagation method for the cinnamomum porrectum to rapid propagation of the cinnamomum porrectum. A water culture method is used for performing cuttage on annual semi-lignified tendertips of the cinnamomum porrectum, and the cutting slips can be promoted to take root in advance; the cuttage rooting efficiency of the cinnamomum porrectum can be obviously improved; the cuttage costis reduced; and the management is convenient.

The invention discloses a Peucedanum praeruptorum rooting culture medium. Every 1000mL of the Peucedanum praeruptorum rooting culture medium contains 0.1-0.3mg of alpha-naphthylacetic acid, 0.1-0.3mgof indoleacetic acid, 0.10-0.18mM of water-soluble neodymium-containing compounds, 0.2-0.8mg of rooting powder, 3-5g of agar powder, 28-34g of cane sugar and the balance of a basic culture medium. Through the rooting culture medium disclosed by the invention, the rooting rate of Peucedanum praeruptorum test-tube plantlets can be increased to 90% or above, and besides, the single-plant rooting number can also be increased to 5 or above, so that the transplanting survival rate of the Peucedanum praeruptorum test-tube plantlets can be greatly increased.

The invention discloses a cutting seedling raising rooting agent for zelkova schneideriana. The cutting seedling raising rooting agent for the zelkova schneideriana, which is provided by the invention, is prepared from the following raw materials in parts by mass: 25 to 50 parts of ABT rooting powder, 1.0 to 3.5 parts of herba portulacae extracting solution, 3 to 6 parts of alkyl glycoside, 1 to 4parts of mint root dry powder, 1 to 4 parts of isomaltotriose, 0.5 to 0.8 part of chrysanthemum extract, 1 to 3 parts of benzoic acid, 0.3 to 1.5 parts of brassin, 2 to 4 parts of saponin, 3 to 6 parts of chelated zinc, 0.3 to 0.6 part of low-sodium salt, 1.2 to 2.5 parts of humic acid, 0.2 to 0.6 part of glyceryl behenate, 1.0 to 2.5 parts of white vinegar, 1.0 to 2.5 parts of tea saponin, 1.5 to 2.5 parts of alga micro mist, 0.4 to 0.6 part of neutral proteinase, 3 to 6 parts of 25 percent carbendazim wettable powder and 0.3 to 0.5 part of L-glutamic acid. The cutting seedling raising rooting agent for the zelkova schneideriana, which is provided by the invention, can be used for advancing the rooting time of a cutting seedling of the zelkova schneideriana and improving rooting efficiency, and the rooting agent can be also used for preventing a nutrition deficiency phenomenon from occurring as a nutrient substance needed by a crop is not supplemented in time after the rooting agentis absorbed by the crop, has balanced nutrients and can be used during all growth periods of the crop.

The invention discloses a cuttage plant rooting agent, which is prepared from the following raw materials by mass: 4 to 10 parts of heteroauxin, 4 to 10 parts of sodium stannate, 4 to 10 parts of naphthoxyacetic acid, 2 to 5 parts of 6-BA, 4 to 10 parts of acetyl choline, 2 to 5 parts of KT-30, 4 to 10 parts of vitamin E, 4 to 10 parts of vitamin B, 4 to 10 parts of inositol, 10 to 20 parts of chitosan, and 5 to 15 parts of folic acid. The cuttage plant rooting agent provided by the invention is particularly suitable for inducing adventive roots during a cutting seedling process of plants difficult to root, is beneficial to promoting cutting and rooting of precious nursery-grown plants difficult to root, shortening the rooting time, improving the quality of the nursery-grown plants and improving the rooting efficiency, and is suitable for being popularized and used on a large scale.

The invention provides a cultivation method of a clonal avocado rootstock. The cultivation method comprises the following steps: (1) cultivation of a nutrient rootstock; (2) grafting of a second rootstock; (3) yellowing treatment; (4) rooting of the second rootstock; and (5) separation and objective scion grafting. The cultivation method utilizes the secondary grafting technology to well solve theproblem of grafting between a rootstock with functions such as disease resistance, and an objective scion. In the method of the invention, the rooting efficiency of the rootstock with functions suchas disease resistance can be promoted by performing the yellowing treatment and cooperating with a rooting nutrient solution suspension bottle infusion method or atomized supply method. In the rootingstep, the second rootstock is subjected to 360 degree wave girdling, and the auxin promotion and a direct rooting or atomization rooting method are used to complete the rooting of the avocado rootstock and the large-scale transplanting of seedlings.

The invention discloses a Phoebe bournei overhead layer seedling growing method. The Phoebe bournei overhead layer seedling growing method comprises the steps of selecting seed trees, selecting branches, causing injuries, performing root promoting treatment, performing overhead layer treatment, performing transplanting, performing transplanting management and the like. The seedling growing methodhas the effects that 1, the rooting efficiency is high and is 44%; 2, little management is needed, no management is needed in the layer period, after transplanting, a root system is supplied, only watering and shading are needed, and the management period just needs one year; 3, the root system is developed, wherein three months after layering is performed, the maximum rooting number is 29, the largest root length is 12.2 cm, and the largest root thickness is 4.85 mm; 4, the transplanting survival rate is high, and the layer transplanting survival rate reaches up to 85%; 5, nursery stock outplanting is rapid, wherein after layers grow one year in a nursery garden, the length is 51+ / -3 cm, and the standard for primary seedlings and secondary seedlings specified by the national forestry industrial standard 'Phoebe bournei cultivation technical regulations' is reached.

The invention relates to the field of plant cultivation, and provides a rapid breeding method of rieger begonia. The method is used for improving the breeding efficiency of the rieger begonia. The provided rapid breeding method of the rieger begonia comprises the steps that S11, young and tender leaves of healthy and strong plants of the rieger begonia are taken as explants; S12, the explants arecut to be divided into multiple primary tissues, and primary adventitious bud induction culture is performed on the primary tissues; S13, the primary tissues with budlet points occurring at the edgesare cut to separate the independent budlets, multiple subculture tissues are also obtained, and subculture adventitious bud induction culture is performed on the independent budlets and the subculturetissues; S14, S13 is repeated to obtain proliferative buds, seedling strengthening culture is performed on the proliferative buds, and the bud bodies are cut after growing to a certain size to obtainrooting seedlings; S15, rooting culture is performed on the rotting seedlings to obtain seedlings of the rieger begonia. In the culture process, and the survival rate of the seedlings is high, more rieger begonia plants can be obtained within the same culture time, so that the breeding efficiency is greatly improved.

The invention discloses a rapid rooting cutting breeding method for sabina chinensis. The rapid rooting cutting breeding method includes the following steps that 1, cutting is carried out in September mid to October early every year, cutting roots are disinfected, regenerated roots are induced, then drying in the shade is carried out, and the product is covered with wet cloth for refreshing; 2, ridges are prepared in a greenhouse, the ridges are filled with cutting media, soil dwelling pests are killed, then disinfection is carried out, tilling is carried out, soil is completely watered with water, and covering is carried out with films; 3, cuttings prepared in the step 1 are inserted into the cutting media in the step 2, the temperature and the humidity are controlled, watering is carried out in a timing mode, cutting is carried out for 2 months, then watering is carried out one time every week, and 0.1% of monopotassium phosphate is sprayed for supplementing nutrients; 4, from the end of September to beginning of October of second year, the height of cutting seedlings reaches 20 cm at the moment, and the seedlings are transplanted. According to the rapid rooting cutting breeding method for the sabina chinensis, a bottleneck of cutting of the sabina chinensis in spring is removed, reproduction of the sabina chinensis is quickened, the rooting efficiency of the sabina chinensis is improved, the survival rate of reproduction is 98% or above, and reproduction plants of the sabina chinensis are increased by one time or above every year.

The invention discloses a rooting agent for raising a cutting seedling of a zelkova serrata. The rooting agent comprises indole butyric acid and talcum powder, wherein the mass of the indole butyric acid in each kilogram of rooting agent is 1300-1600 mg. The rooting agent for raising the cutting seedling of the zelkova serrata can shorten time for rooting and can improve rooting efficiency, a zelkova serrata scion branch processed by the rooting agent can grow axillary buds after around 22 days of cutting, after three days of the buding of the scion branch, little amount of culluses are generated at a lower cutting opening of the scion branch; along with the rising of the temperature, after about 40 days, all axillary buds grow out, spires are grown, and about 80% of scion branch generates calluses; the rooting of the processed scion branch is mainly based on calluses in an intensive and bursting manner; the quantity of roots is large, branch roots are strong and the number of the branch root can even be hundreds.

The invention discloses a method for evidently increasing Litsea cubeba cluster bud rooting rate. The method includes the steps of firstly, separating Litsea cubeba cluster buds to obtain single buds;secondly using an anti-browning agent perform soaking pretreatment on the base cut of each single bud, wherein the anti-browning agent comprises astaxanthin; thirdly, inoculating the pretreated single buds to a seedling strengthening culture medium to perform seedling strengthening culture; fourthly, transferring the single buds after the seedling strengthening culture to a primary culture mediumto perform primary rooting culture to induce root primordium formation; fifthly, transferring the single buds after the primary rooting culture to a subculture medium to perform rooting subculture toobtain rooted seedlings, wherein the subculture medium comprises Litsea cubeba root tip tissue extract. The method has the advantages that cluster bud death caused by tissue browning during culture can be reduced greatly, the rooting efficiency and root growth speed of the Litsea cubeba cluster buds are increased evidently, Litsea cubeba rapid propagation efficiency is increased, and Litsea cubeba industry development is promoted.

The invention provides a cutting propagation method of clematis armandii. A proper clematis armandii cutting medium is found, a reasonable medium component proportion is set, by matching various cutting propagation details such as corresponding proper cutting depth, node number of cutting slips, leaf number, growth hormone with proper soaking concentration and components and the like, the rooting efficiency and survival rate are improved, the propagation efficiency of the clematis armandii is improved, the propagation yield of the clematis armandii is increased, the production cost is reduced, and the requirements of propagation production and landscape application of clematis armandii resources are met.

The invention discloses a method of propagating Petunia hybrida by layering; the method comprises: vertically cutting a hard portion of a base of a branch of Petunia hybrida to obtain a wound, with epidermis cut through to expose xylem, applying a rooting agent to the wound, covering the wound with substrate until the covered portion roots, separating the lower end of the rooting portion of the branch from a parent body after rooting, and performing transplanting. The method has the advantages of high seedling growth speed, early flowering, high survival rate, good convenience of operation, and low cost.

The invention discloses a method for cultivating a tree-shaped Chinese rose stock. The method includes the following steps: a first step, providing a substrate, compressing the substrate into a substrate block having one side with at least one fixing hole, putting the substrate and the substrate block into a flowerpot, allowing the fixing hole to be exposed out of the substrate, arranging the flowerpot in a seedling bed, and paving at least one gauze net and a blank gauze cloth on the seedling bed; a second step, selecting a rose branch as a stock scion; a third step, dipping a base of the stock scion into a rooting solution, inserting the stock scion into the fixing hole of the substrate block, and allowing the upper end of the stock scion to contact with the gauze net; a fourth step, using the blank gauze cloth to block light within 10-14 days after the stock scion is inserted, keeping the humidity at 70-75%, keeping the temperature at 22-25 DEG C, removing the blank gauze cloth, keeping the humidity at 50-55%, keeping the temperature at 26-30 DEG C during illumination, and keeping the temperature at 8-10 DEG C during dark; and a fifth step, cultivating the stock scion into a tree-shaped Chinese rose stock after cultivation for 4-5 months, taking the tree-shaped Chinese rose stock out from the seedling bed, removing the flowerpot, and transplanting the tree-shaped Chinese rose stock in a humus substrate.

The invention provides a special rooting agent for forestry seedlings. The agent comprises, in parts by mass, the following components: 8-12 parts of auxin, 20-50 parts of catechol, 18-26 parts of copper tartrate, 7-26 parts of naphthylacetic acid, 0.1-5 parts of 3-aminopropanol, 20-30 parts of diuron, 50-70 parts of erucyl amide, 1-10 parts of salicylic acid, 0.5-10 parts of benayl aminopurine, 30-60 parts of cytokinin and 50-90 parts of deionized water. The special rooting agent for the forestry seedlings can effectively improve the rooting efficiency, preparation is simple and operation iseasy.

The invention discloses a selenium-rich pear seedling raising device and a seedling raising method thereof, and relates to the technical field of pear seedling raising. The selenium-rich pear tree seedling raising method mainly comprises the steps of cutting slip selection, cutting slip treatment, seedling raising treatment, seedling raising management and the like, a used seedling raising device comprises a water storage box and a placement box, the two ends of the placement box are connected with the upper ends of the two sides of the water storage box through lifting mechanisms, a plurality of planting mechanisms are arranged on the placement box, and each planting mechanism comprises a planting pot with an upward opening; the lower end of the planting pot penetrates through the lower end of the placing box to extend downwards, a first hollowed-out net layer is arranged around the side wall of the middle of the planting pot, a circle of second hollowed-out net layer is arranged at the lower end of the side wall of the planting pot, and multiple water leakage holes are formed in the bottom of the planting pot. According to the method, the defects in the prior art are overcome, the pear tree cutting seedling raising time can be effectively shortened, meanwhile, the survival rate of pear seedlings is increased, the seedling raising process of the pear seedlings is conveniently operated and controlled, the method is suitable for large-scale cultivation of the pear seedlings, and the pear seedling raising efficiency is improved.

The invention discloses a method for growing seedlings of passion fruit. The seeds of passion fruit are soaked in a potassium permanganate solution with a mass concentration of 1-3%, and then washed with sterile water to obtain sterile seeds; Bacteria seeds are inoculated in the primary culture medium, and the primary culture is carried out to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants, and inoculated in the secondary culture medium, and the subculture is carried out to obtain Rootless seedling: described rootless seedling is transplanted in the rooting medium, carries out rooting culture, obtains passion fruit seedling. The method for growing seedlings by tissue culture of the invention has high rooting efficiency and high survival rate, and can maintain good quality of plants.

The invention discloses a rooting basal medium (BPM) and a cultivation method thereof, characterized in that the components of the MS basal medium are improved, rooting inducers (NAA, activated carbon) are added, and culture conditions are adjusted. , the rooting rate in the bottle can reach 100% in 14 days, so that the transplanting survival rate can reach more than 90%, which is 12%~34% higher than the current report. It only takes 45 days from inoculation to transplanting to survive, which not only improves the rooting efficiency of the tissue-cultured seedlings of Lime plum, but also has the characteristics of neat and robust seedlings, saving costs and high efficiency, and shortening the cultivation cycle of tissue-cultured seedlings of Lime plum. Chemical production provides the means to rapidly produce high-quality seedlings.

The invention discloses rooting basic media (BPM) special for prunus maritima marshall tissue culture seedlings and a cultural method thereof. The rooting basic media (BPM) special for the prunus maritima marshall tissue culture seedlings and the cultural method thereof are characterized in that by means of the supporting measures such as improving MS basic medium components, adding rooting inductive agents (NAA and activated carbon) and adjusting culture conditions, the rooting rate in a bottle for 14 d can reach 100%, the transplanting survival rate can reach over 90%, and the transplanting survival rate is increased by 12%-34% than that of a report at present. 45 d is only needed from inoculation to transplanting survival, the efficiency of inducing rooting of the prunus maritima marshall tissue culture seedlings is improved, and cultured seedlings are in order and strong. The method for rapidly and efficiently inducing rooting of the prunus maritima marshall tissue culture seedlings has the advantages that the cost is reduced, the efficiency is high, the culture period of the prunus maritima marshall tissue culture seedlings is shortened, and the method for rapidly producing high quality seedlings is provided for scale production.

The invention discloses a method for improving the rooting efficiency of muskmelon seedlings. The method comprises the steps of soaking muskmelon seeds in a potassium permanganate solution of which the mass concentration is 1 to 3 percent, and then washing the muskmelon seeds by using sterile water to obtain sterile seeds; inoculating the sterile seeds into a primary culture medium for primary culture to obtain sterile seedlings; taking cotyledons and hypocotyledonary axis of the sterile seedlings as explants, inoculating the sterile seedlings into a secondary culture medium for secondary culture to obtain rootless seedlings; transplanting the rootless seedlings into a rooting culture medium for rooting culture to obtain muskmelon seedlings. A tissue culture seedling growth method has the advantages of high rooting efficiency and high survival rate, and good quality of a plant can be maintained.