Method for evidently increasing Litsea cubeba cluster bud rooting rate

A technology of Litsea cubeba root and Litsea cubeba, which is applied in the field of plant tissue culture and in vitro rapid propagation, can solve the problems of restricting the in vitro efficiency of Litsea cubeba, easy browning of base incision, long time for rooting, etc., to achieve Improve rapid propagation efficiency, increase rooting efficiency and root growth speed, and promote the effect of development

Active Publication Date: 2020-01-31
HUNAN NUOZ BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, when the clustered buds of Litsea cubeba are induced to take root, there are problems that the base incision is easy to brown, the rooting rate is not high, and the time required for rooting is long, which seriously restricts the efficiency of rapid propagation of Litsea cubeba in vitro

Method used

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  • Method for evidently increasing Litsea cubeba cluster bud rooting rate
  • Method for evidently increasing Litsea cubeba cluster bud rooting rate
  • Method for evidently increasing Litsea cubeba cluster bud rooting rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 antibrowning agent

[0031] Take 190g of sucrose and dissolve in 900ml of distilled water, then add 30mg of silver nitrate, then add 250mg of astaxanthin pre-dissolved in 10ml of ethanol, and finally distilled water to 1 liter to obtain anti-browning agent A.

Embodiment 2

[0032] The preparation of embodiment 2 antibrowning agent

[0033] Dissolve 180g of sucrose in 900ml of distilled water, then add 10mg of silver nitrate, then add 200mg of astaxanthin pre-dissolved in 10ml of ethanol, and finally distill the water to 1 liter to obtain anti-browning agent B.

[0034] The preparation of embodiment 2 antibrowning agent

[0035] Take 200g of sucrose and dissolve in 900ml of distilled water, then add 50mg of silver nitrate, then add 300mg of astaxanthin pre-dissolved in 10ml of ethanol, and finally distilled water to 1 liter to obtain anti-browning agent C.

Embodiment 4

[0036] The preparation of embodiment 4 litsea cubeba root tip tissue extract

[0037] Take 7g of the root tip tissue of Litsea Cubeba seedlings (the length of the material is 0.5cm above the root tip), grind it into powder with liquid nitrogen and collect it in a 50ml centrifuge tube, add 23ml of 80% methanol, mix well and place it at 40°C Incubate in a water bath for 5 hours, then centrifuge at 3000rpm / min for 13 minutes, absorb the supernatant, remove the methanol in the supernatant in a rotary evaporator, then add 0.7g vitamin C to the supernatant, distilled water to volume 50ml, Litsea cubeba root tip tissue extract A, placed in a 4°C refrigerator for later use.

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Abstract

The invention discloses a method for evidently increasing Litsea cubeba cluster bud rooting rate. The method includes the steps of firstly, separating Litsea cubeba cluster buds to obtain single buds;secondly using an anti-browning agent perform soaking pretreatment on the base cut of each single bud, wherein the anti-browning agent comprises astaxanthin; thirdly, inoculating the pretreated single buds to a seedling strengthening culture medium to perform seedling strengthening culture; fourthly, transferring the single buds after the seedling strengthening culture to a primary culture mediumto perform primary rooting culture to induce root primordium formation; fifthly, transferring the single buds after the primary rooting culture to a subculture medium to perform rooting subculture toobtain rooted seedlings, wherein the subculture medium comprises Litsea cubeba root tip tissue extract. The method has the advantages that cluster bud death caused by tissue browning during culture can be reduced greatly, the rooting efficiency and root growth speed of the Litsea cubeba cluster buds are increased evidently, Litsea cubeba rapid propagation efficiency is increased, and Litsea cubeba industry development is promoted.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and in vitro rapid propagation, and more specifically relates to a method for significantly improving the rooting rate of litsea cubeba clustered shoots. Background technique [0002] Litsea cubeba (Litsea cubeba) is a unique industrial raw material and spice tree species in southern my country. The essential oil extracted from its fruit has a high content of citral and is widely used in medicine, food, cosmetics and other fields. The establishment of the tissue culture and rapid propagation system of Litsea cubeba can not only promote the development of Litsea cubeba industry, but also lay the foundation for the basic research of Litsea cubeba. [0003] However, at present, there are problems such as easy browning of base incision, low rooting rate and long rooting time during rooting induction of litsea cubeba cluster buds, which seriously restricts the efficiency of in vitro rapid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈昊张付豪裴莹王阳
Owner HUNAN NUOZ BIOLOGICAL TECH CO LTD
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