Non-tube quick reproduction method for seedings of jatropha curcas L.
A tung tree and fast technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of difficulty in meeting the demand for quantity and quality of tung tree seedlings, high production costs, low survival rate, etc. Improve the efficiency of rapid propagation, save germplasm material, and the effect of large amount of material
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[0026] Both the embodiment and the comparative example adopt 1-year-old tung tree branches to intercept the micro-stem section for rapid propagation.
[0027]Sterilization treatment: Remove the leaves from the harvested branches, soak them in a disinfectant solution [5% sodium hypochlorite (NaClO), 0.1% Tween-20] for 20 minutes, and then rinse them with tap water 5 times; cut the branches into shorter lengths of about 15-20 cm Stem segments were soaked in 70% ethanol for 10 minutes, then soaked in disinfectant solution for 15 minutes, and rinsed with sterile water for 5 times to thoroughly wash away residual sodium hypochlorite.
[0028] Prepare cytologin indole butyric acid (IBA) solution; the concentrations are 0.2, 0.5, 1.0, 2.0 (mg / L).
[0029] Prepare nutrient solution: prepare 1 / 2 MS basic medium, pH 5.8-6.0, see Table 1 for specific formula.
[0030] Table 1
[0031]
[0032]
[0033] Non-test tube rapid propagation: cut the sterilized stems into 2-3cm long micr...
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