Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Non-tube quick reproduction method for seedings of jatropha curcas L.

A tung tree and fast technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of difficulty in meeting the demand for quantity and quality of tung tree seedlings, high production costs, low survival rate, etc. Improve the efficiency of rapid propagation, save germplasm material, and the effect of large amount of material

Inactive Publication Date: 2010-05-19
SUN YAT SEN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are following problems in these two kinds of methods: (1) rely on natural growth, the speed of seed propagation of tung tree is slow, the efficiency is low
It usually takes 1 to 3 years for tung tree to mature and bear fruit, and it enters the rich production period in the fifth year. The small amount of tung tree currently planted in China is difficult to provide enough seeds for the construction of large-scale planting bases in a short period of time.
(2) Seed propagation and sowing methods are not conducive to the maintenance of good traits
(3) Cuttage propagation seedling raising method needs 20~40cm long, the small tung tree branch of about 2cm in diameter as the propagation unit material (Yuan Lichun, Xu Zhongzhi, Wu Kui etc. Jatropha planting technology [J]. Yunnan Agricultural Science and Technology, 2006, ( 1): 58.), tung tree belongs to shrub or small tree, and the small amount of tung tree currently planted in China is also difficult to provide enough germplasm materials to meet the needs of building large-scale planting bases and afforestation on barren hills
Although the rapid propagation of test-tube seedlings improves the individual reproduction speed compared with the traditional propagation and cultivation method, and avoids the influence and restriction of individual reproduction by natural factors such as seasons and regions, but the operation is complicated and cumbersome, and the plants need a strict aseptic growth environment. High cost and low survival rate when transplanting test-tube plantlets to soil
In addition, during the tissue culture process, test-tube plantlets are exposed to high concentrations of cytokinin, cytokinin and other plant growth regulators for a long time, which is prone to mutation, resulting in the loss of some excellent traits
Therefore test-tube seedling rapid propagation method is also difficult to meet the demand of large-scale afforestation project to the quantity and quality of tung tree seedlings

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Non-tube quick reproduction method for seedings of jatropha curcas L.
  • Non-tube quick reproduction method for seedings of jatropha curcas L.
  • Non-tube quick reproduction method for seedings of jatropha curcas L.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Both the embodiment and the comparative example adopt 1-year-old tung tree branches to intercept the micro-stem section for rapid propagation.

[0027]Sterilization treatment: Remove the leaves from the harvested branches, soak them in a disinfectant solution [5% sodium hypochlorite (NaClO), 0.1% Tween-20] for 20 minutes, and then rinse them with tap water 5 times; cut the branches into shorter lengths of about 15-20 cm Stem segments were soaked in 70% ethanol for 10 minutes, then soaked in disinfectant solution for 15 minutes, and rinsed with sterile water for 5 times to thoroughly wash away residual sodium hypochlorite.

[0028] Prepare cytologin indole butyric acid (IBA) solution; the concentrations are 0.2, 0.5, 1.0, 2.0 (mg / L).

[0029] Prepare nutrient solution: prepare 1 / 2 MS basic medium, pH 5.8-6.0, see Table 1 for specific formula.

[0030] Table 1

[0031]

[0032]

[0033] Non-test tube rapid propagation: cut the sterilized stems into 2-3cm long micr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a non-tube method for fast breeding aleurite seedling, comprising: taking disinfected stem carrying axillary bud of 2-3cm length as explant; enclosing its morphological upper notch, immersing its morphological lower notch in indolebutyric acid solution, cutting it in sand disk, dressing nutritional solution to keep sand disk wet, culturing it for 16. 2-20. 2 days under conditions of 25-28 Deg. C, 70-80% of relative humidity, 2000-3000 lx lighting strength and light period of 12 hour lighting / 12 hour dark, and getting rooting and integral plant. The invention is characterized by fast breeding, free from influence of national factors such as season and region, low variation rate, simple process and low cost.

Description

technical field [0001] The invention relates to the field of rapid plant propagation, in particular to a non-test tube rapid propagation method for tung tree seedlings. Background technique [0002] Jatropha curcas L., also known as Jatropha Curcas, Jatropha curcas, Green Jade Tree, Hibiscus Tree, Cream Tung, Black Soap, Bright Tung, Stink Tung, Green Tung Wood, Yellow Swollen Tree, Water Paint, Tung Oil Tree , False peanut tree, Hemp Han, etc., are Euphorbiaceae (Euphorbiaceae) Jatropha (Jatropha) deciduous shrubs or small trees, native to America, now widely distributed in tropical, subtropical and dry hot valley areas, in my country Mainly distributed in Guangdong, Hainan, Fujian, Taiwan, Guangxi, Yunnan, Guizhou, Sichuan and other places (Editorial Committee of Flora of China. Flora of China [M]. Beijing: Science Press, 147-148.). Tung tree has high economic value. The whole plant can be used as medicine, astringent, slightly cold, and poisonous (Kong Lingyi, Min Zhida, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 王兆玉徐增富廖文波罗莉
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products