Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for improving the rapid propagation efficiency of apple rootstock tissue culture seedlings

A technique for tissue culture seedlings and apples, which is applied in the field of double fast and one recovery to improve the efficiency of tissue culture seedlings of apple rootstocks, can solve the problems of unfavorable promotion of virus-free seedlings of apple rootstocks, long culture period, low efficiency, etc. Efficiency, increase growth rate and value-added coefficient, shorten the effect of time

Active Publication Date: 2022-04-22
SHANDONG AGRICULTURAL UNIVERSITY +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current apple rootstock tissue culture technology generally has problems such as low efficiency and long cultivation period. The value-added coefficient of about 4 times cannot meet the rapid multiplication requirements of large-scale production, wasting a certain amount of manpower and material resources, and is not conducive to the removal of apple rootstocks. Promotion of poisonous vaccines

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for improving the rapid propagation efficiency of apple rootstock tissue culture seedlings
  • A method for improving the rapid propagation efficiency of apple rootstock tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the method for improving the rapid propagation efficiency of apple rootstock tissue culture seedlings

[0051] 1. Promoting germination:

[0052] At the beginning of May 2020, the apple rootstock 12-2, which was self-selected by the inventor through in situ breeding technology, was selected to grow robust shoots, cut it into stems with new shoots of about 3 cm, rinsed with clean water for one hour, and placed in the Soak it in 75% alcohol for 30s on the ultra-clean workbench that has been sterilized by ultraviolet light for 30 minutes, then place it in 0.05% sodium hypochlorite solution for disinfection for 10 minutes, and wash it with sterile water 3 times, 30 seconds each time. Use a sterilized scalpel to cut an oblique incision about 0.5 cm from the bottom of each stem segment, and inoculate it on the induction medium. The medium is based on 1L MS medium, and the concentration of other components is sucrose 30g / L, agar 7.5g / L, 6-BA 0.6mg / L, IBA 0.2mg / L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving the rapid propagation efficiency of apple rootstock tissue culture seedlings, which comprises the steps of explant inoculation, primary rapid subculture, revertive subculture, secondary rapid subculture and time-lapse subculture. When adopting the method of the present invention, when the time-reducing subculture is adopted, the 20-25 day multiplication coefficient is about 6.11 times, and the height is about 5.23 cm, and the same value-added coefficient can be maintained for more than 5 generations, which not only greatly improves the apple rootstock group. The growth speed and value-added coefficient of the cultured seedlings are improved, and the time of traditional apple rootstock tissue culture is shortened by 1 / 3-1 / 2, which can speed up the high-quality and efficient production of apple rootstock tissue culture seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for improving the rapid propagation efficiency of apple rootstock tissue culture seedlings by double fast and one recovery. Background technique [0002] Apple cultivation mainly exists in the form of grafting rootstocks to scions to cultivate seedlings. Rootstocks have an important impact on the growth and development of apples, fruit quality and yield, and resistance to various biotic and abiotic stresses. Therefore, efficient breeding of apple rootstocks has always been the focus of research at home and abroad. [0003] Tissue culture technology has opened up a new way for the rapid propagation of apple rootstock. At present, a large number of apple rootstocks and new varieties have established rapid propagation systems through tissue culture. Wang Miaomiao and others found that the ratio of plant growth regulators suitable for multiplication and cultiv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 毛云飞陈春玲胡艳丽曹颖贺然魏钰樊金龙尹伊君崔雪丽龚丽娟关洛非孙楠王悦张桐源郭翎沈向
Owner SHANDONG AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com