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Tissue-culture rapid propagation method for pyrus betulaefolia bunge

A technique for tissue culture rapid propagation and duplication of pears, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve problems such as differences in the cultivation methods of medium formulas, shorten the seedling raising period, reduce the breeding cost, and reduce labor and labor costs. The effect of reagent consumable costs

Active Publication Date: 2014-11-12
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have been successfully reported on different plants, but there are great differences in the medium formulations and culture methods used by different plants

Method used

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  • Tissue-culture rapid propagation method for pyrus betulaefolia bunge
  • Tissue-culture rapid propagation method for pyrus betulaefolia bunge
  • Tissue-culture rapid propagation method for pyrus betulaefolia bunge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: The effect of different auxins on the callus induction effect of Duli pear stem segment.

[0038] This embodiment is based on the stem section of the robust test-tube clustered seedlings obtained by culturing in MS proliferation medium for 25-35 days, and the height of the test-tube clustered seedlings is 1 cm to 3 cm.

[0039] The formula for callus induction culture is as follows:

[0040] Treatment 1: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+0.5mg / L NAA

[0041] Treatment 2: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.0mg / L NAA

[0042] Treatment 3: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.5mg / L NAA

[0043] Treatment 4: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+2.0mg / L NAA

[0044] Treatment 5: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+0.5mg / L IBA

[0045] Treatment 6: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.0mg / L IBA

[0046] Treatment 7: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.5mg / L IBA

[0047] Treatment 8: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+2.0mg / L IBA...

Embodiment 2

[0053] Embodiment 2: the influence of the rooting medium of different formulations on rooting effect.

[0054] This embodiment is based on the stem section of the robust test-tube clustered seedlings obtained by culturing in MS proliferation medium for 25-35 days, and the height of the test-tube clustered seedlings is 1 cm to 3 cm.

[0055] The rooting medium formula is as follows:

[0056] Treatment 1: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+0.5mg / L NAA

[0057] Treatment 2: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.0mg / L NAA

[0058] Treatment 3: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.5mg / L NAA

[0059] Treatment 4: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+2.0mg / L NAA

[0060] Treatment 5: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+0.5mg / L IBA

[0061] Treatment 6: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.0mg / L IBA

[0062] Treatment 7: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.5mg / L IBA

[0063] Treatment 8: 1 / 2MS+15g / l sucrose+5.0g / L agar powder+2.0mg / L IBA

[0064] Treatm...

Embodiment 3

[0071] Embodiment 3: The effect of NAA and IBA compound treatment on the rooting of Duli pear.

[0072] This embodiment is based on the robust test-tube bushy seedlings obtained by culturing in MS proliferation medium for 25-35 days, and the seedling height of the test-tube seedlings is 1 cm to 3 cm.

[0073] callus induction medium

[0074] 1 / 2MS+15g / l sucrose+5.0g / L agar powder+1.5mg / L NAA

[0075] 1 / 2MS+15g / l sucrose+5.0g / L agar powder+2.0mg / L NAA

[0076] rooting medium

[0077] 1 / 2MS+15g / L sucrose+5.0g / L agar powder+1.5mg / L IBA

[0078] 1 / 2MS+15g / L sucrose+5.0g / L agar powder+2.0mg / L IBA

[0079] experiment method:

[0080] The robust test-tube plantlets were divided into 4 groups, respectively cultured in the dark for 7 days on the callus induction medium of 1 / 2MS+15g / l sucrose+5.0g / L agar powder and NAA of different concentrations, and then transferred to 1 / 2MS+15g / L sucrose+5.0g / L agar powder and supplemented with different concentrations of IBA in the rooting me...

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Abstract

The invention discloses a tissue-culture rapid propagation method for pyrus betulaefolia bunge. The tissue-culture rapid propagation method for the pyrus betulaefolia bunge comprises disinfecting on explants, inducing buds, propagating test tube seedlings, inducing calluses, inducing rooting and transplanting exercising seedlings. During the process of inducing rooting, the calluses are firstly induced and then the rooting is induced; obtained rooting test tube seedlings are performed seedling exercising in a triangular flask, then transferred to perlite to domesticate the exercising seedling and finally transplanted. Compared with the traditional method, the tissue-culture rapid propagation method for the pyrus betulaefolia bunge has the advantages of being high in rooting rate and survival rate of transplanting, high in propagation rate, low in cost, convenient to popularize, good in genetic stability and suitable for the pyrus betulaefolia bunge.

Description

technical field [0001] The invention relates to the rapid propagation of plant tissue by tissue culture technology, in particular to a method for tissue culture rapid propagation of Du pear. Background technique [0002] Pear is a plant of the genus Pyrus L. in the Rosaceae family and the subfamily Pomoideae. Pear is an important fruit tree, ranking third in my country's fruit production, after apples and citrus. Pears are propagated by grafting in production, and rootstock is an important basis for fruit tree grafting and is closely related to the production of fruit trees. The selection of excellent rootstocks can regulate tree vigor, prolong the fruiting period, and even regulate fruit quality. Du pear (Pyrus betulaefolia Bunge.) is one of the main rootstocks of pears. Due to its strong suitability, drought tolerance, deep and well-developed roots, resistance to barrenness, and strong saline-alkali resistance, it can be used in neutral soil and saline-alkaline soil. It ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 李晓刚蔺经王中华杨青松李慧王宏盛宝龙常有宏
Owner JIANGSU ACAD OF AGRI SCI
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