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Prescription of rooting medium for cultivating cowberry

A technology for rooting medium and tissue culture, applied to plant cells and other directions, can solve the problems of long rooting time, high management cost, low reproduction coefficient, etc., and achieve the effects of high rooting efficiency, simple management and high survival rate.

Inactive Publication Date: 2009-09-16
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bilberry cutting propagation takes a long time for rooting, about 60 days, high management cost, and low reproduction coefficient, so tissue culture factory propagation is an effective way to ensure the supply of seedlings
At present, research materials on tissue culture of lingonberry are occasionally found in domestic and foreign literatures. Domestic literature records that the tissue culture lingonberry seedlings are rooted by cuttings outside the bottle, but the required environmental conditions are strict, the control is relatively difficult, and the rooting situation is not ideal.
There are no effective research reports on rooting medium formulations suitable for factory production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Cut off the lower callus of the 3-4cm high multiplication seedlings of the lingonberry sharpblue (the current production variety) that has been multiplied and cultivated for 30 days in the ultra-clean workbench, and inoculate it on the prepared sterilized K2 medium, each bottle Inoculate about 20 roots and culture them under weak light (illumination intensity <1000Lex) for 7-10 days, and you can see that more than 95% of the proliferating seedlings grow 2-5 white fibrous roots with a root length of 0.2-1cm, and then transfer the culture bottle Carry out light cultivation on a cultivation frame with light intensity exceeding 1500Lex. After 7 days, the length of white fibrous roots is 0.5-2cm, obviously thickened, and the leaves are dark green. At this time, the rooted seedlings that grow robustly can be taken out and transplanted.

Embodiment 2

[0028] The 3-4cm high multiplication seedlings of the lingonberry Darrow (the current production variety) that have been multiplied and cultivated for 30 days are cut off the lower part of the callus in the ultra-clean workbench, and inoculated onto the prepared sterilized k2 medium, each bottle Inoculate about 20 roots and culture them under weak light (illumination intensity <1000Lex) for 7-10 days, and you can see that more than 95% of the proliferating seedlings grow 2-5 white fibrous roots with a root length of 0.2-1cm, and then transfer the culture bottle Carry out light cultivation on a cultivation rack with light intensity exceeding 1500Lex. After 7 days, the length of white fibrous roots is 0.5-2cm, which is obviously thickened and the leaves are dark green. Seedling.

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Abstract

A prescription of rooting medium for cultivating cowberry consists of macro element, trace element, iron salt and organic component, etc. of different components and concentration. The rooting medium has high routing efficiency above 95percent. The period for routing cultivation is short and it can be transplanted in less than 20 days requiring no hardening off, and is simple to manage after being transplanted with a survival rate as high as 95percent. Besides, the component of the culture medium is ordinary and easy to confect without special compounds.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rooting medium formula for cranberry tissue culture in the process of factory seedling cultivation. Background technique [0002] Bilberry is a new generation of fruit with the fastest development in the world in recent years. It has unique nutritional and health functions. It can be eaten fresh, processed into jam and wine, extracted from natural anthocyanins, and used as raw materials for medicines. At present, it has been proved that the biologically active substances in the lingonberry fruit have the functions of improving eyesight, eliminating eye fatigue, and enhancing vision; they have obvious therapeutic effects on various malignant tumors such as rectal cancer; Wisdom, the role of preventing Alzheimer's disease; can reduce the cholesterol level in the body, regulate blood sugar concentration, prevent and treat cardiovascular disease and type II diabetes; c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 李凌李政
Owner SOUTHWEST UNIV
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